We narrowed to 923 results for: tac promoter

1. SBP-GFP-Tac

   Plasmid

   #162505

   Purpose

   Expresses GFP tagged Tac in a RUSH vector in mammalian cells

   Depositor

   Lei Lu

   Article

   Sun et al J Biol Chem. 2020 Aug 21. pii: RA120.014

   Insert

   Tac (IL2RA Human)
   

   Use

   Tags

   GFP and SBP

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Jan. 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pBrain-GFP-TACC3KDP(L566,567A)-shTACC3

   Plasmid

   #69117

   Purpose

   Dual-promoter plasmid to express knockdown-proof human TACC3 (L566A and L567A substitutions) tagged with EGFP along with shRNA to knock down endogenous TACC3 in mammalian cells.

   Depositor

   Stephen Royle

   Article

   Gutierrez-Caballero et al Biol Open. 2015 Jan 16;4(2):170-9. doi:

   Inserts

   Transforming Acidic Coiled Coil protein 3 (TACC3 Human)
   shTACC3
   

   Use

   RNAi

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   LL (566-567aa) mutated to AA. Silent mutations i…

   Promoter

   CMV

   Available Since

   Feb. 19, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. SBP-GFP-Tac-TC

   Plasmid

   #162506

   Purpose

   Expresses GFP tagged partial length Tac (TMD and cytosolic tail) in RUSH vector in mammalian cells

   Depositor

   Lei Lu

   Article

   Sun et al J Biol Chem. 2020 Aug 21. pii: RA120.014

   Insert

   GFP and SBP tagged partial length Tac (TMD and cytosolic tail) (IL2RA Human)
   

   Use

   Tags

   GFP and SBP

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Jan. 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pBrain-GFP-TACC3KDP∆678-681-shTACC3

   Plasmid

   #69115

   Purpose

   Dual-promoter plasmid to express knockdown-proof human TACC3 (amino acids 678-681 deleted) tagged with EGFP along with shRNA to knock down endogenous TACC3 in mammalian cells.

   Depositor

   Stephen Royle

   Article

   Gutierrez-Caballero et al Biol Open. 2015 Jan 16;4(2):170-9. doi:

   Inserts

   Transforming Acidic Coiled Coil protein 3 (TACC3 Human)
   shTACC3
   

   Use

   RNAi

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Deletion of 678-681aa region. Silent mutations i…

   Promoter

   CMV

   Available Since

   Oct. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. GFP-Tac-STC

   Plasmid

   #162490

   Purpose

   Expresses partial length Tac (stem region, TMD and cytosolic tail) with GFP tag in mammalian cells

   Depositor

   Lei Lu

   Article

   Sun et al J Biol Chem. 2020 Aug 21. pii: RA120.014

   Insert

   partial length Tac (stem region, TMD and cytosolic tail) (IL2RA Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Tac is in partial length with its stem region, TM…

   Promoter

   Available Since

   Jan. 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. GFP(N-glyc)-Tac-TC

   Plasmid

   #162496

   Purpose

   Expresses partial length Tac (TMD and cytosolic tail) with GFP tag in the N-terminus in mammalian cells, and there is one N-glycosylation site in GFP tag.

   Depositor

   Lei Lu

   Article

   Sun et al J Biol Chem. 2020 Aug 21. pii: RA120.014

   Insert

   partial length Tac (TMD and cytosolic tail) (IL2RA Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Tac is in partial length with its TMD and cysotol…

   Promoter

   Available Since

   Jan. 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. GFP-Tac-STC(5A)

   Plasmid

   #162491

   Purpose

   Expresses non-O-glycosylated partial length Tac (stem region, TMD and cytosolic tail) with GFP tag in mammalian cells

   Depositor

   Lei Lu

   Article

   Sun et al J Biol Chem. 2020 Aug 21. pii: RA120.014

   Insert

   partial length Tac (stem region with mutations, TMD and cytosolic tail) (IL2RA Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Tac is in partial length with its stem region, TM…

   Promoter

   Available Since

   Jan. 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pSF-p15A-tac- YFP

   Plasmid

   #186570

   Purpose

   p15A ori, lacI, Ptac promoter, AmpR. Kringle YFP (Yellow Fluorescence Protein) expression

   Depositor

   Teresa de Diego Puente

   Article

   Lozano Terol et al Front Microbiol. 2021 Jun 21;12:682001.

   Insert

   YFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   tac promoter

   Available Since

   Nov. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pSF-pMB1'- tac-YFP

   Plasmid

   #186566

   Purpose

   pMB1' ori, lacI, Ptac promoter, AmpR. Kringle YFP (Yellow Fluorescence Protein) expression

   Depositor

   Teresa de Diego Puente

   Article

   Lozano Terol et al Front Microbiol. 2021 Jun 21;12:682001.

   Insert

   YFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   tac promoter

   Available Since

   Nov. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pBrain-GFP-TACC3KDP∆682-688-shTACC3

    Plasmid

    #69116

    Purpose

    Dual-promoter plasmid to express knockdown-proof human TACC3 (682-688aa ch-TOG binding site deleted) tagged with EGFP along with shRNA to knock down endogenous TACC3 in mammalian cells.

    Depositor

    Stephen Royle

    Article

    Gutierrez-Caballero et al Biol Open. 2015 Jan 16;4(2):170-9. doi:

    Inserts

    Transforming Acidic Coiled Coil protein 3 (TACC3 Human)
    shTACC3
    

    Use

    RNAi

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Deletion of amino acids 682-688 (region containin…

    Promoter

    CMV

    Available Since

    Oct. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. GFP-Cstem-Tac(5A)

    Plasmid

    #162497

    Purpose

    Expresses GFP tagged Tac mutant in mammalian cells. The five O-glycosylation sites in the stem region are mutated, and the stem region of CD8a is inserted between GFP and Tac(5A).

    Depositor

    Lei Lu

    Article

    Sun et al J Biol Chem. 2020 Aug 21. pii: RA120.014

    Insert

    CD8a stem region and Tac full length with mutated stem region (IL2RA, CD8A Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    The five threonine residues are mutated into alan…

    Promoter

    Available Since

    Jan. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. GFP-Cstem(7A)-Tac(5A)

    Plasmid

    #162499

    Purpose

    Expresses GFP tagged Tac mutant in mammalian cells. The five O-glycosylation sites in the stem region are mutated, and the stem region of CD8a with mutations is inserted between GFP and Tac(5A).

    Depositor

    Lei Lu

    Article

    Sun et al J Biol Chem. 2020 Aug 21. pii: RA120.014

    Insert

    CD8a stem region with mutations and Tac full length with mutated stem region (IL2RA, CD8A Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    The five theronine and two serine residues are mu…

    Promoter

    Available Since

    Jan. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pcDNA3-HA-PHLPP1(N-terminal truncation)-deltaC

    Plasmid

    #22931

    Purpose

    Mammalian expression of a HA tagged, N-terminal truncation of human PHLPP1 containing a deletion of the PDZ binding motif (delta C)

    Depositor

    Alexandra Newton

    Article

    Gao et al Mol Cell. 2005 Apr 1. 18(1):13-24.

    Insert

    PHLPP1-deltaC (PHLPP1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    PHLPP-deltaC, deletion of PDZ binding domain--the…

    Promoter

    CMV

    Available Since

    Feb. 4, 2010

    Availability

    Academic Institutions and Nonprofits only

14. 8xCTS1_deltaC55G-hutRNApro_BsmBIplch-sgRNA_14nt Buffer_delta-hutRNApro_SV40polyA

    Plasmid

    #124543

    Purpose

    Cloning vector for tRNA-released sgRNA. BsmBI flanked placeholder for spacer cloning.

    Depositor

    Tudor Fulga

    Article

    Knapp et al Nat Commun. 2019 Apr 2;10(1):1490. doi:

    Insert

    deltaC55G-hutRNApro_BsmBIplch-sgRNA_14nt Buffer_delta-hutRNApro
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    d11-41 C55G, d11-41

    Promoter

    Minimal ADE

    Available Since

    April 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. 8xCTS1_deltaC55A-hutRNApro_BsmBIplch-SAMsgRNA_14nt Buffer_delta-hutRNApro_SV40polyA

    Plasmid

    #124539

    Purpose

    Cloning vector for tRNA-released synergistic activation mediator gRNA. BsmBI flanked placeholder for spacer cloning.

    Depositor

    Tudor Fulga

    Article

    Knapp et al Nat Commun. 2019 Apr 2;10(1):1490. doi:

    Insert

    deltaC55A-hutRNApro_BsmBIplch-SAMsgRNA_14nt Buffer_delta-hutRNApro
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    d11-41 C55A, d11-41

    Promoter

    Minimal ADE

    Available Since

    April 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. 8xCTS1_deltaC55A-hutRNApro_BsmBIplch-sgRNA_14nt Buffer_delta-hutRNApro_SV40polyA

    Plasmid

    #124544

    Purpose

    Cloning vector for tRNA-released sgRNA. BsmBI flanked placeholder for spacer cloning.

    Depositor

    Tudor Fulga

    Article

    Knapp et al Nat Commun. 2019 Apr 2;10(1):1490. doi:

    Insert

    deltaC55A-hutRNApro_BsmBIplch-sgRNA_14nt Buffer_delta-hutRNApro
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    d11-41 C55A, d11-41

    Promoter

    Minimal ADE

    Available Since

    April 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. 8xCTS1_deltaC55G-hutRNApro_BsmBIplch-SAMsgRNA_14nt Buffer_delta-hutRNApro_SV40polyA

    Plasmid

    #124538

    Purpose

    Cloning vector for tRNA-released synergistic activation mediator gRNA. BsmBI flanked placeholder for spacer cloning.

    Depositor

    Tudor Fulga

    Article

    Knapp et al Nat Commun. 2019 Apr 2;10(1):1490. doi:

    Insert

    deltaC55G-hutRNApro_BsmBIplch-SAMsgRNA_14nt Buffer_delta-hutRNApro
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    d11-41 C55G, d11-41

    Promoter

    Minimal ADE

    Available Since

    April 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. mEGFP-N1-YAPS127A-deltaCC

    Plasmid

    #166459

    Purpose

    Expresses fusion of mEGFP and YAPS127A-deltaCC (delta298-359)

    Depositor

    Shuguo Sun

    Article

    Yu et al Mol Cell. 2021 Feb 13. pii: S1097-2765(2

    Insert

    mEGFP, YAPS127A-deltaCC (YAP1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pJM105: pTac RFP on pCM66T

    Plasmid

    #74746

    Purpose

    pCM66T with promoter pTac driving RFP expression (maxRFP = BBa_E1010)

    Depositor

    Mary Lidstrom

    Insert

    RFP (BioBrick part number BBa_E1010)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pTac

    Available Since

    May 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAttB-Tub-HA-WnkDeltaCC

    Plasmid

    #203972

    Purpose

    Fly transformation vector expressing indicated Wnk variant under tubulin promoter for ubiquitous expression

    Depositor

    Andreas Jenny

    Insert

    Wnk (Wnk Fly)
    

    Use

    Tags

    HA

    Expression

    Insect

    Mutation

    deletion of coiled coil domains 1 and 2

    Promoter

    Available Since

    Oct. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge