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pHH0103_TJP1-1/1-SV1/2

Plasmid

#91321

Purpose

Protein expression and purification of human SH3 domain construct TJP1-1/1-SV1/2

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

TJP1-1/1-SV1/2 (TJP1 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_SH3RF2-1/3-SV1/2

Plasmid

#91322

Purpose

Protein expression and purification of human SH3 domain construct SH3RF2-1/3-SV1/2

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

SH3RF2-1/3-SV1/2 (SH3RF2 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_MPP1-1/1-IS2/2

Plasmid

#91307

Purpose

Protein expression and purification of human SH3 domain construct MPP1-1/1-IS2/2

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

MPP1-1/1-IS2/2 (MPP1 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_EPS8L1-1/1-IS2/2

Plasmid

#91315

Purpose

Protein expression and purification of human SH3 domain construct EPS8L1-1/1-IS2/2

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

EPS8L1-1/1-IS2/2 (EPS8L1 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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p2xFlag CMV2 Lats1-PY1+2 mutant

Plasmid

#18975

Depositor

Marius Sudol

Article

Oka et al J Biol Chem. 2008 Oct 10;283(41):27534-2

Insert

Large tumor suppressor kinase 1 PY1+2 mutant (LATS1 Human)

Tags

2xFlag

Expression

Mammalian

Mutation

PY1 mutant, (Tyrosine 376 changed to Alanine) and…

Available Since

Sept. 8, 2008

Availability

Academic Institutions and Nonprofits only
pEGFP C3-Lats1-PY1+2 mutant

Plasmid

#19054

Depositor

Marius Sudol

Article

Oka et al J Biol Chem. 2008 Oct 10;283(41):27534-2

Insert

Large tumor suppressor kinase 1, N mutant (LATS1 Human)

Tags

EGFP

Expression

Mammalian

Mutation

PY1 mutant, (Tyrosine 376 changed to Alanine) and…

Available Since

Sept. 8, 2008

Availability

Academic Institutions and Nonprofits only
pCDNA3-SREBP-2-GFP (A1134-1135)

Plasmid

#204203

Purpose

Mammalian expression of SREBP-2

Depositor

Allan Weissman

Article

Yang et al J Biol Chem. 2023 Oct 12;299(12):105346.

Insert

SREBP-2 (SREBF2 Human)

Tags

T7x3 and eGFP

Expression

Mammalian

Mutation

Gly to Ala 1134, 1135

Available Since

April 8, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3 Flag beta-2-adrenergic-receptor

Plasmid

#14697

Depositor

Robert Lefkowitz

Article

Tang et al Proc Natl Acad Sci U S A. 1999 Oct 26. 9

Insert

B2AR (ADRB2 Human)

Tags

Flag

Expression

Mammalian

Available Since

June 19, 2007

Availability

Academic Institutions and Nonprofits only

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pCDNA4TO R99A CoV-2 nsp1 3xFLAG

Plasmid

#176061

Purpose

Expresses R99A CoV2 nsp1 in mammalian cells. This contains a C-terminal 3xFLAG fusion to nsp1.

Depositor

Britt Glaunsinger

Article

Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

Insert

CoV-2 nsp1 (ORF1ab SARS-CoV-2)

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 15, 2021

Availability

Academic Institutions and Nonprofits only

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Lenti-guide-puro mBltp1 (KIAA1109) - 2

Plasmid

#198490

Purpose

lentiviral stable expression of mBltp1 (KIAA1109) gRNA 2

Depositor

Suzanne Pfeffer

Article

Dhekne et al Elife. 2023 Oct 24;12:e87098. doi: 10.75

Insert

mBltp1 (4932438A13Rik Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

April 21, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-mDlx-GCaMP6f-Fishell-2

Plasmid

#83899

Purpose

GCaMP6f expression in forebrain GABA-ergic interneurons under the control of the mDlx enhancer element

Depositor

Gordon Fishell

Article

Dimidschstein et al Nat Neurosci. 2016 Oct 31. doi: 10.1038/

Has Service

AAV Retrograde, AAV1, and AAV9

Insert

GCaMP6f

Use

AAV

Expression

Mammalian

Promoter

mDlx

Available Since

Oct. 31, 2016

Availability

Academic Institutions and Nonprofits only

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Axin GID 1-2 LP/pCS2MT

Plasmid

#16300

Depositor

Peter Klein

Article

Hedgepeth et al Mol Cell Biol. 1999 Oct . 19(10):7147-57

Insert

Axin GID 1-2 LP (axin1.L Frog)

Use

Vertebrate expression

Tags

5x myc

Mutation

GSK-3 interaction domain aa 320-429 with L to P m…

Available Since

Dec. 21, 2007

Availability

Academic Institutions and Nonprofits only
SPELL-ITSN1(DH-PH) Component 2

Plasmid

#170492

Purpose

SPELL ITSN1(DH-PH) component 2. myc-FRB-ITSN(C-lobe). Split at loop 4 of DH domain.

Depositor

Klaus Hahn

Article

Dagliyan et al Nat Commun. 2018 Oct 2;9(1):4042. doi: 1

Insert

myc, ITSN1(C-lobe), FRB

Expression

Mammalian

Available Since

July 27, 2021

Availability

Academic Institutions and Nonprofits only

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pVRC8400-SARS-CoV-2-RBD-AVI

Plasmid

#160476

Purpose

Expresses SARS-CoV-2 RBD domain with single chain Fc, HRV3C protease cleavage site, and AVI tag

Depositor

Peter Kwong

Article

Zhou et al Cell Rep. 2020 Oct 27;33(4):108322. doi:

Insert

SARS-CoV-2-RBD

Tags

AVI, HRV3C, and scFc

Expression

Mammalian

Available Since

Oct. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pVRC8400-SARS-CoV-2-NTD-AVI

Plasmid

#160475

Purpose

Expresses SARS-CoV-2 NTD domain with single chain Fc, HRV3C protease cleavage site, and AVI tag

Depositor

Peter Kwong

Article

Zhou et al Cell Rep. 2020 Oct 27;33(4):108322. doi:

Insert

SARS-CoV-2-NTD

Tags

AVI, HRV3C, and scFc

Expression

Mammalian

Available Since

Oct. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pVRC8400-SARS-CoV-2-S2P-AVI

Plasmid

#160474

Purpose

Expresses SARS-CoV-2 S2P protein with single chain Fc, HRV3C protease cleavage site, and AVI tag

Depositor

Peter Kwong

Article

Zhou et al Cell Rep. 2020 Oct 27;33(4):108322. doi:

Insert

SARS-CoV-2-S2P

Tags

AVI, HRV3C, and scFc

Expression

Mammalian

Available Since

Oct. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pSLQ2806-2 pHR: U6-Sasgv2TRE3G CMV-mCherry

Plasmid

#84250

Purpose

Expresses optimized Sa sgTRE3G gRNA with a mCherry fluorescent marker

Depositor

Stanley Qi

Article

Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

Insert

Sa sgTRE3G

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

mouse U6

Available Since

Nov. 11, 2016

Availability

Academic Institutions and Nonprofits only

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pHH0103_DOCK5-1/1-IS1/2

Plasmid

#91303

Purpose

Protein expression and purification of human SH3 domain construct DOCK5-1/1-IS1/2

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

DOCK5-1/1-IS1/2 (DOCK5 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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Beta-2-adrenergic receptor-YFP

Plasmid

#67146

Purpose

Beta-2-adrenergic receptor tagged with YFP on carboxyl terminus

Depositor

Catherine Berlot

Article

Hynes et al J Biol Chem. 2004 Oct 15;279(42):44101-1

Insert

Beta-2-adrenergic receptor-YFP (ADRB2 Aequorea victoria, Human)

Tags

YFP

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 6, 2015

Availability

Academic Institutions and Nonprofits only

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Beta-2-adrenergic receptor-mRFP

Plasmid

#67145

Purpose

Beta-2-adrenergic receptor tagged with mRFP on carboxyl terminus

Depositor

Catherine Berlot

Article

Hynes et al J Biol Chem. 2004 Oct 15;279(42):44101-1

Insert

Beta-2-adrenergic receptor-mRFP (ADRB2 Human, Discosoma sp.)

Tags

mRFP

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 6, 2015

Availability

Academic Institutions and Nonprofits only

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