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Plasmid#188488PurposegRNA plasmid with neo resistance expressing a double cutting single gRNA targeting tdTomato fluorescent protein sites 166 & 892.DepositorInserttdTomato sgRNA
UseCRISPRTagsExpressionMammalianMutationNonePromoterU6Available sinceAug. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
rtTA-sg-tdTomato90/816
Plasmid#188487PurposegRNA plasmid with neo resistance expressing a double cutting single gRNA targeting tdTomato fluorescent protein sites 90 & 816.DepositorInserttdTomato sgRNA
UseCRISPRTagsExpressionMammalianMutationNonePromoterU6Available sinceAug. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
tdTomato-β-actin3'UTR2
Plasmid#183717PurposetdTomato with fragment nucleotides 441-676 of beta-actin 3'UTR inserted in 3'UTRDepositorInserttdTomato with fragment (nucleotides 441-676) of mouse Actb 3'UTR
UseLentiviralTagsExpressionMutationPromoterUbiCAvailable sinceMay 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
tdTomato--β-actin CDS2
Plasmid#183714PurposetdTomato with fragment nucleotides 398-818 of beta-actin coding sequence inserted in 3'UTRDepositorInserttdTomato with fragment (nucleotides 398-818) of mouse Actb coding dsequence
UseLentiviralTagsExpressionMutationPromoterUbiCAvailable sinceMay 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
tdTomato-β-actin3'UTR1
Plasmid#183716PurposetdTomato with fragment nucleotides 1-440 of beta-actin 3'UTR inserted in 3'UTRDepositorInserttdTomato with fragment (nucleotides 1-440) of mouse Actb 3'UTR
UseLentiviralTagsExpressionMutationPromoterUbiCAvailable sinceMay 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pX459-sg-tdTomato166/892
Plasmid#179919PurposeDouble cutting Cas9 plasmid with puromycin resistance and a single guide RNA targeting tdTomato fluorescent protein sites 166 and 892.DepositorInserttdTomato sgRNA
UseCRISPRTags2A-Puro and 3XFLAGExpressionMammalianMutationPromoterU6Available sinceApril 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
ymTq2-tdTomato pDRF1-GW
Plasmid#168068PurposeFRET fusion between ymTq2 and tdTomato for use in yeastDepositorInsertymTq2-tdTomato
UseTagsExpressionYeastMutationPromoterAvailable sinceJune 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBS-f1(-)-TOMM20-dTomato
Plasmid#115922Purposedonor to insert dTomato at the TOMM20 locus in human cellsDepositorInsertTOMM20-dTomato
UseDonor templateTagsdTomatoExpressionMutationPromoterAvailable sinceMarch 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBS-f1(+)-SEC61B-dTomato
Plasmid#115913Purposedonor to insert dTomato at the SEC61B locus in human cellsDepositorInsertSEC61B-dTomato (SEC61B Human)
UseDonor templateTagsdTomatoExpressionMutationPromoterAvailable sinceMarch 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBS-f1(+)-TOMM20-dTomato
Plasmid#115914Purposedonor to insert dTomato at the TOMM20 locus in human cellsDepositorInsertTOMM20-dTomato (TOMM20 Human)
UseDonor templateTagsdTomatoExpressionMutationPromoterAvailable sinceMarch 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
YSBE415: pTOP_ACA1_Vc_HOG1 terminator
Plasmid#122434PurposePlasmid for tagging proteins in Cryptococcus neoformans; C-terminal fragment of Venus proteinDepositorInsertC-terminal fragment of Venus protein
UseTagging proteins in cryptococcus neoformansTagsExpressionMutationPromoterAvailable sinceMay 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
YSBE420:pNEO_CAC1_Vn_HOG1 terminator
Plasmid#122701PurposePlasmid for tagging proteins in Cryptococcus neoformans; N-terminal fragment of Venus proteinDepositorInsertN-terminal fragment of Venus protein
UseTagging proteins in cryptococcus neoformansTagsExpressionMutationPromoterAvailable sinceMay 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
YSBE416: pTOP_CAC1_Vn_HOG1 terminator
Plasmid#122435PurposePlasmid for tagging proteins in Cryptococcus neoformans; N-terminal fragment of Venus proteinDepositorInsertN-terminal fragment of Venus protein
UseTagging proteins in cryptococcus neoformansTagsExpressionMutationPromoterAvailable sinceMay 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
YSBE418:pNAT_ACA1_Vc_HOG1 terminator
Plasmid#122700PurposePlasmid for tagging proteins in Cryptococcus neoformans; C-terminal fragment of Venus proteinDepositorInsertC-terminal fragment of Venus protein
UseTagging proteins in cryptococcus neoformansTagsExpressionMutationPromoterAvailable sinceApril 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
FLAG-Atoh1 del3
Plasmid#96913PurposeMurine Atoh1 with amino acids 214-305 removedDepositorInsertAtoh1 (Atoh1 Mouse)
UseTags2xFLAGExpressionMammalianMutationDeletion of amino acids 214-305PromoterAvailable sinceAug. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
FLAG-Atoh1 del4
Plasmid#96914PurposeMurine Atoh1 with amino acids 306-347 removedDepositorInsertAtoh1 (Atoh1 Mouse)
UseTags2xFLAGExpressionMammalianMutationDeletion of amino acids 306-347PromoterAvailable sinceAug. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
FLAG-Atoh1 S334A
Plasmid#96915PurposeMurine Atoh1 with S334A mutationDepositorInsertAtoh1 (Atoh1 Mouse)
UseTags2xFLAGExpressionMammalianMutationSerine 334 changed to AlaninePromoterAvailable sinceAug. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
FLAG-Atoh1 S309A
Plasmid#96916PurposeMurine Atoh1 with S309A mutationDepositorInsertAtoh1 (Atoh1 Mouse)
UseTags2xFLAGExpressionMammalianMutationSerine 309 changed to AlaninePromoterAvailable sinceAug. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
FLAG-Atoh1 S325A
Plasmid#96926PurposeMurine Atoh1 with S325A mutationDepositorInsertAtoh1 (Atoh1 Mouse)
UseTags2xFLAGExpressionMammalianMutationSerine 325 changed to AlaninePromoterAvailable sinceAug. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
FLAG-Atoh1 S328A
Plasmid#96927PurposeMurine Atoh1 with S328A mutationDepositorInsertAtoh1 (Atoh1 Mouse)
UseTags2xFLAGExpressionMammalianMutationSerine 328 changed to AlaninePromoterAvailable sinceAug. 4, 2017AvailabilityAcademic Institutions and Nonprofits only