We narrowed to 2,683 results for: PREP

1. CLYBL_Bxb1-GA_LP_Dual_TC

   Plasmid

   #229776

   Purpose

   CLYBL targeting construct with the Bxb1-GA landing pad cassette.

   Depositor

   Richard Davis

   Article

   Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

   Insert

   FRT-mScarlet-attP(GA)-PuroR-F3
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   pGK

   Available since

   Feb. 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pIVT-Flp-2A-BleoR

   Plasmid

   #232752

   Purpose

   IVT template for making bicistronic modRNA encoding Flippase and Zeocin resistance gene

   Depositor

   Kate Galloway

   Article

   Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

   Inserts

   FlpO
   BleoR
   

   Use

   In vitro transcription

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 17, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pIVT-iCre

   Plasmid

   #232773

   Purpose

   IVT template for making modRNA encoding iCre

   Depositor

   Kate Galloway

   Article

   Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

   Insert

   iCre
   

   Use

   Cre/Lox; In vitro transcription

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 17, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. Bxb1-GA_CAG-mStayGold

   Plasmid

   #229802

   Purpose

   Bxb1-GA donor plasmid for integrating a transcription unit for constitutive expression of mStayGold

   Depositor

   Richard Davis

   Article

   Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

   Insert

   mStayGold
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Feb. 19, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAD-GamS

   Plasmid

   #99246

   Purpose

   Expresses 6xHis-tagged GamS from T7lac promoter in the E.coli Tuner (DE3) strain. See notes below for important strain information.

   Depositor

   Jeff Hasty

   Article

   Didovyk et al ACS Synth Biol. 2017 Aug 10. doi: 10.102

   Insert

   gamS (with C-terminal 6xHis tag)
   

   Use

   Tags

   6xHis tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7lac

   Available since

   Oct. 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLenti_dCas9-KRAB-MeCP2_Blast

   Plasmid

   #221501

   Purpose

   Lentiviral construct for expression of the dCas9-KRAB-MeCP2 CRISPRi vector

   Depositor

   Matthew Meyerson

   Article

   Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

   Insert

   dCas9-KRAB-MeCP2-T2A-Blast
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Cas9 mutations to make dCas9=D10A+D839A+H840A+N86…

   Promoter

   CMV

   Available since

   July 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pHPHS800

   Plasmid

   #228240

   Purpose

   Bxb1 attB_wildtype site and SpCas9-hygro for integration into Bxb1 attP_wildtype landing pad (eg. pHPHS232). Includes pEF1 and attP_GA for second round of recombination

   Depositor

   Arvind Rasi Subramaniam

   Article

   Nugent et al bioRxiv [Preprint]. 2024 Jul 26:2024.07.

   Insert

   Cas9
   

   Use

   Tags

   Hygromycin phosphotransferase

   Expression

   Mammalian

   Mutation

   Promoter

   none

   Available since

   Dec. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET15b-RvLEAMshort

   Plasmid

   #218122

   Purpose

   Expresses truncated RvLEAM protein under IPTG induction in bacterial host cells.

   Depositor

   Ci Ji Lim

   Article

   Abe et al bioRxiv [Preprint]. 2024 Feb 11:2024.02.

   Insert

   RvLEAMshort
   

   Use

   Tags

   6xHIS

   Expression

   Bacterial

   Mutation

   Truncated form (58-181aa)

   Promoter

   T7

   Available since

   April 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. PP_072_pAAV_hSyn_DIO-LR-Voltron2-P2A-LR-CheRiff-HA

   Plasmid

   #230988

   Purpose

   Co-expressing membrane-localized Voltron2 and membrane-localized CheRiff.

   Depositor

   Adam Cohen

   Article

   Wong-Campos et al bioRxiv [Preprint]. 2023 May 26:2023.05.

   Insert

   Cre-dependent, membrane-localized optopatch for neuronal dendrites
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hSyn

   Available since

   June 5, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-CAG-FlpX-mKate2.0-N2cG

    Plasmid

    #234711

    Purpose

    pAAV transfer plasmid with CAG promoter driving Flp-dependent expression of mKate2.0 and N2c G protein separated by a T2A linker

    Depositor

    David Ng

    Article

    Mendelsohn et al bioRxiv [Preprint]. 2024 Sep 18:2024.08.

    Insert

    mKate2.0 T2A N2cG
    

    Use

    AAV

    Tags

    T2A

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    April 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAD-LyseR

    Plasmid

    #99244

    Purpose

    For autolysate production in E. coli BL21-Gold (DE3) strain: constitutively expresses phage lambda endolysin (gene R)

    Depositor

    Jeff Hasty

    Article

    Didovyk et al ACS Synth Biol. 2017 Aug 10. doi: 10.102

    Insert

    phage lambda gene R (R phage lambda)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    beta-lactamase promoter

    Available since

    Oct. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. OSCA1.2-peGFP-N1

    Plasmid

    #228585

    Purpose

    Expresses OSCA1.2-eGFP in mammalian cells

    Depositor

    Huanghe Yang

    Article

    Lowry et al bioRxiv [Preprint]. 2024 Sep 3:2024.02.0

    Insert

    hyperosmolality-gated Ca2+ permeable channel (OSCA) 1.2 (AT4G22120 Mustard Weed)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAGD335

    Plasmid

    #219499

    Purpose

    pAGD248 derivative with the retron Eco1 msr, sfGFP flanked by 2 BsaI sites , and the rest of the retron recombineering operon (Eco1 RT, CspRect, MutL-E32K) downstream of the pBad promoter

    Depositor

    Seth Shipman

    Article

    Gonzalez-Delgado et al bioRxiv [Preprint]. 2023 Jul 17:2023.07.

    Insert

    Eco1 msr, sfGFP, Eco1 RT, CspRecT, MutLE32K
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pBAD

    Available since

    June 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. Bxb1-GT_CAG-mRuby2

    Plasmid

    #229801

    Purpose

    Bxb1-GT donor plasmid for integrating a transcription unit for constitutive expression of mRuby2

    Depositor

    Richard Davis

    Article

    Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

    Insert

    mRuby2
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Feb. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMON-HsNMT1

    Plasmid

    #67475

    Purpose

    Expression of human N-myristoyltransferase 1 for N-myristoylation of ARF family proteins in bacteria

    Depositor

    Richard Kahn

    Article

    Van Valkenburgh et al Methods Enzymol. 2002;344:186-93.

    Insert

    HsNMT1 (NMT1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

16. Bxb1-GA_CAG-EGFP

    Plasmid

    #229799

    Purpose

    Bxb1-GA donor plasmid for integrating a transcription unit for constitutive expression of EGFP

    Depositor

    Richard Davis

    Article

    Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

    Insert

    EGFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Feb. 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pNDL-60

    Plasmid

    #161720

    Purpose

    In vitro transcription construct for production of cytosolic acvr2b-CIB1N mRNA

    Depositor

    Adam Cohen

    Article

    McNamara et al bioRxiv [Preprint]. 2024 Apr 12:2024.04.

    Insert

    cytosolic acvr2b-CIB1N (acvr2b Zebrafish)
    

    Use

    In vitro transcription

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. Bxb1-GA Donor_hEF1a

    Plasmid

    #229779

    Purpose

    Bxb1-GA donor plasmid for the cloning of payloads <20 kb with hEF1a promoter for expressing landing pad enrichment marker

    Depositor

    Richard Davis

    Article

    Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAAV-Fon/ConN2cG

    Plasmid

    #234709

    Purpose

    pAAV transfer plasmid with Ef1a promoter driving Cre- and Flp-dependent expression of G protein for rabies N2c delta G

    Depositor

    David Ng

    Article

    Mendelsohn et al bioRxiv [Preprint]. 2024 Sep 18:2024.08.

    Insert

    Fon Con N2cG
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Ef1a

    Available since

    March 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pIVT-PuroR

    Plasmid

    #232753

    Purpose

    IVT template for making modRNA encoding the Puromycin resistance gene

    Depositor

    Kate Galloway

    Article

    Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

    Insert

    PuroR
    

    Use

    In vitro transcription

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 17, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge