Tips for Titering Your Lentiviral Preps
Type
Blog Post
...freshly collected lentiviral prep may be significantly higher than the same prep that has been stored in the...painstakingly cared for your packaging cell line, prepped your DNA, transfected and harvested your lentivirus...determine the amount of virus you actually have in your prep. Taking time to properly titer your virus will not...qPCR for viral RNA. Measuring p24 levels in a viral prep is straightforward and rapid as there are several...universal primers can be used to titer any lentiviral prep that shares that specific feature. Primers targeting...overestimate the amount of infectious virus in a prep as the measurement will include defective particles...following transduction with serial dilutions of a viral prep. This method is particularly useful when vectors...