...viral prep with multiple serotypes pAAV-hSyn-GRAB_OTmut AAV1 Biosensor Yulong Li New viral prep pAAV-CAG-NIR-GECO2G...Fyhn New viral prep AAV-hSyn-Ribo-jGCaMP8m AAV1 Biosensor Marianne Fyhn New viral prep pAAV-Ef1a-fDIO... viral prep pAAV_BiSSTe10_dTomato_nlsdTomato AAV-PHPeB Control Gordon Fishell New viral prep pAAV_... viral prep pAAV_BiCHATe27_dTomato_nlsdTomato AAV-PHPeB Control Gordon Fishell New viral prep pAAV_BiCHATe27...New viral prep pAAV_BiVIPe4_dTomato_nlsdTomato AAV-PHPeB Control Gordon Fishell New viral prep pAAV_... viral prep pAAV_BiLAMP5e3_ChR2_mCherry AAV-PHPeB Optogenetics Gordon Fishell New viral prep pAAV_...New viral prep pAAV_BiPVe4_ChR2_mCherry AAV-PHPeB Optogenetics Gordon Fishell New viral prep pAAV_BiPVe3...

... new preps available in the Addgene viral vector repository! This includes the first Addgene prep packaged...viral prep pAAV_hSyn-DIO-PdCO-mScarlet-WPRE AAV1, AAV5 Optogenetics Ofer Yizhar New viral prep with...Lin Tian New viral prep pAAV-hSyn-GRAB-gDA3m AAV1 Biosensors Yulong Li New viral prep pGP-AAV-GFAP-iGABASnFR2...part of the BRAIN Armamentarium Collection. These preps drive expression of either Cre recombinase or a ...Recombinases Stanley Thomas Carmichael New viral prep pAAV-EF1a-fDIO-Cre AAV5 Recombinases Esteban ... AAV PHP.eB Recombinases Jonathan Ting New viral prep AiP13779 - pAAV-AiE0779m_3xC2-minBG-iCre(R297T... AAV PHP.eB Recombinases Jonathan Ting New viral prep AiP13781 - pAAV-AiE0452h-minBG-iCre(R297T)-BGHpA...

...have added the prep to your cart and checked out.  Prices for Packaged on Request viral preps will be available...Request prep and run the same quality control assays we run with all of our in-stock viral preps: ddPCR...on Request prep 6–9 weeks after placing your order.  Can I order Packaged on Request preps through a distributor... and ordering a Packaged on Request viral vector prep.    Excited? So are we! This expansion will ... select their serotype of choice, pick what size prep they’d like, and submit the request. Request details...details can be accessed within your Addgene account. Preps will be available for request in three different...be 7x1012, the same titers found in our in-stock preps. If a request has technical considerations that ...

...over 100,000 viral vector preps!   Figure 1: A) Cumulative AAV preps available in the Addgene ...Cumulative AAV preps available at Addgene from 2016–2023 by type of tool. B) AAV preps distributed by ...AAV preps for the seven most popular serotypes available from Addgene from 2016–2023. B) AAV preps for...jGCaMP7, and jGCaMP8 viral preps distributed per year compared to other AAVs preps from 2016–2023. B) Inset...prioritizing which preps to include in our service allows us to make ready-to-use viral preps that are available...both to produce and to verify the quality of the prep. This was a large factor in Addgene’s decision to.... From 2016–2023, we shared 93,491 viral vector preps with researchers in 45 different countries — and...

...lentivirus (with many CRISPR tools included among the preps that are currently available) and adeno associated...faster by producing, titering, and testing viral preps for them. At the same time, we’re developing ourselves...scientists and accelerate research." What viral preps are available now? There are a wide variety of tools...already available from Addgene as ready-to-use viral preps and we’ll be continually expanding our collection...provide a brief rundown of what lentiviral and AAV preps are available below, but you can always head over...we’re starting the Viral Service with lentiviral preps for the delivery of Cas9 endonuclease, Cas9 nickase... be available soon. We’re also distributing AAV preps with particular serotypes that can be used to deliver...

...freshly collected lentiviral prep may be significantly higher than the same prep that has been stored in the...painstakingly cared for your packaging cell line, prepped your DNA, transfected and harvested your lentivirus...determine the amount of virus you actually have in your prep. Taking time to properly titer your virus will not...qPCR for viral RNA. Measuring p24 levels in a viral prep is straightforward and rapid as there are several...universal primers can be used to titer any lentiviral prep that shares that specific feature. Primers targeting...overestimate the amount of infectious virus in a prep as the measurement will include defective particles...following transduction with serial dilutions of a viral prep. This method is particularly useful when vectors...

...streak for single colonies, sub-culture, and prep with a DNA prep kit or your lab's favorite in-house protocol...plasmid yields from your prep, try our tips below! How to optimize your plasmid prep We'll discuss the following...plasmid DNA and lead to degradation of your prep. Many plasmid prep kits recommend performing an extra wash...updated on March 21, 2018. Most of the time, plasmid prepping is a breeze. You get your stab from Addgene, streak... multiple techniques for a particularly stubborn prep. Check the bacterial strain Increase the volume...This strain consistently gives good, high quality preps due to its endA1 recA1 relA1 genotype, as these ...terminal repeats. We use NEB Stable to transform and prep high quality DNA that contains these elements. Be...

...or a pre-made viral prep library. Addgene’s pooled libraries are typically DNA preps, but we do distribute...libraries as pre-made viral preps. If you can’t (or don’t want to) make viral preps in your lab, there are ...pooled library lentiviral prep. B) Usage of a pooled library lentiviral prep for experiments. Pooled library...are custom viral prep services that can prepare the libraries for you.  Starting from plasmids requires ... use! In this post, I’ll walk you through how to prep and transduce your pooled library.  Pooled libraries... library lentiviral preps are generally aliquoted to reduce the number of freeze-thaw cycles needed when...functional titer is determined, larger lentiviral preps can be used for screening-scale transduction of ...

...excited to provide ready-to-use CRISPR lentiviral preps to researchers across the globe. Why provide CRISPR...in science, getting a high titer Cas9 lentivirus prep takes a bit of time and effort that, for many groups... protocol only to find that the low titer of her prep makes it unuseable. With the large size of the SpCas9...producing, titering, testing, and providing lentiviral preps of select lentiviral vectors from the repository...We perform test infections with all of our viral preps and host our expression data (western blots or fluorescent...gRNA-containing lentivirus, we are providing lentiviral preps containing highly requested gRNAs including those...library, ensure gRNA representation in the viral prep, infect target cells, and, finally, conduct the ...

...to provide ready-to-use viral preps. Here are some of the new viral preps from recent months: Expanded...INTRSECT viral preps from the Deisseroth Lab now available. Find all the Deisseroth viral preps here Coming...highlight a subset of the new plasmids and viral preps in the repository through our hot plasmids articles.... Serotypes include AAV1, and AAV9. Find viral preps here pAAV-hSyn-Cre-P2A-dTomato is now available... AAV5 and AAVrg. Find the Cre-expressing viral preps here AAV-CAG-dLight1.3b from the Lin Tian Lab ...Lab is now available as a ready to use viral prep in serotype AAV5! Get dLight1.3b here Looking for a...apoptosis. Now available as a ready to use viral prep in serotype AAV5. Find AAV-flex-taCasp3-TEVp here...

...to provide ready-to-use viral preps. Here are some of the new viral preps from recent months: Axon targeting...INTRSECT viral preps available! We are expanding our inventory of ready to use viral preps from the Deisseroth...highlight a subset of the new plasmids and viral preps in the repository through our hot plasmids articles...pathways The CRISPR corner New ready-to-use viral preps Mapping antibody escape in SARS-CoV-2 by mutational...GCaMP8 vectors in serotypes AAV1 and AAV5. AAV9 preps and more AAV1 versions will be released soon. Visualize...project vectors available as ready-to-use viral preps with more on the way. S5E2 regulatory element containing...GCaMP6f are now available as a ready-to-use viral prep in serotype AAV1. Distinguish dendritic neurites...

...State Ladder: Homemade Plasmid Prep Penn State Ladder: Commercial Plasmid Prep Kit Amount 1000 Lanes 1000...significantly cheaper. When using a homemade plasmid prep protocol, it cost less than $10 to make 1000 lanes...bp or the 1 kb ladder. Using a commercial plasmid prep kit increases the costs to $20-30 per ladder: still...DNA ladder? You can find the Tan Lab’s DNA ladder prep protocol here and the pPSU1 and the pPSU2 plasmids...Addgene.org Use the Kit-Free Alkaline Lysis Plasmid Miniprep Protocol Learn How to Analyze Your Restriction...

...AAV and lentiviral preps we have released in the last few months: Lentiviral preps for expressing SARS-CoV...lentiviral preps to speed up COVID-19 research even further. The following lentiviral preps are now available...dependent Cre and FLPo And more!  New lentiviral preps SARS-CoV-2 protein expression The Krogan lab quickly... Expression of the SARS-CoV-2 ORF3a Lentiviral preps for expressing the E protein (pLVX-EF1alpha-SARS-CoV... pooled library is now available as a lentiviral prep. It's a human CRISPR inhibition library that inhibits...inhibition screening. Find the Dolcetto lentiviral prep here Control AAV Control AAV allow researchers to...of our viral vectors blog posts Find tips for preparing AAVs Download our viral vectors 101 eBook Resources...

...References ASAPbio, Preprint Resource Center: https://asapbio.org/preprint-info ASAPbio, Preprints and Rapid Communication...Transparency Another major benefit of preprints is their openness. Preprints can be read freely by anyone and...of the benefits of preprints. Some worry about faulty work being posted as preprints, assuming that peer...the manuscript prior to submission. Preprints at Addgene Preprints are all about sharing scientific knowledge...described in a preprint. In fact, over 1,500 plasmids in our catalog either list a bioRxiv preprint as the source...now-published article was a preprint. Depositing plasmids that were used in a preprint provides all the same... position working with preprints in life sciences. He also posted two preprints of his own research during...

...viral preps. Here are some of the new viral preps from recent months: New INTRSECT viral preps available...available as a ready-to-use viral prep in AAV1!  New ready-to-use AAV prep from Hongkui Zeng's lab are available...highlight a subset of the new plasmids and viral preps in the repository through our hot plasmids articles...are expanding our inventory of ready to use viral preps from the Deisseroth lab INTRSECT project. Check ... lab, now available in a ready to use AAV5 viral prep.   Flp-dependent DREADDs from the Gether lab, now...

...ve found helpful for my interviews in industry. Prepare stories to tell in your interview The STAR interview...interview has started. Instead, I preferred to prepare stories to answer interview questions. Story telling...starting place for drafting my stories. In total, I prepare 5-6 stories for an interview, since one story usually...stories helped me remember them for the interview.  Prepare a list of questions to ask your interviewers You... as much as they are interviewing you, so come prepared with questions to ask. With a quick Google search...and company culture.  It’s also a good idea to prepare 1-2 unique questions for each interviewer by looking...deliverable. Find 10 steps to a perfect science talk Be prepared for potential technical difficulties. Have your...

... other before proceeding to library prep. Even with this “pre-prep” normalization, it is also often the...enhancing the efficiency and simplicity of library prep. As part of our mission, we’ve been working with...Traditional NGS workflows are limited by costly library prep, as each sample requires an individual library. ...plexWell™ workflow is a sequential “pooled library prep”, in which a relatively large number of samples ...variation is problematic for conventional NGS library prep methods because the yield or quality of individual...many labs. However, the high costs of sample preparation for high-throughput next generation (NGS) sequencing...Addgene to develop and apply our plexWell™ Library Preparation Technology for NGS-based sequencing and confirmation...

...through how we produce both our AAV preps and our lentiviral preps.   Viruses are generated using standard...order to generate high quality preparations. After production, all virus preps are titered and subjected to...Addgene has a growing catalogue of viral preps available to request. In this post, we’ll walk you through...pAAV-Ef1a-mCherry-IRES-Cre was a gift from Karl Deisseroth (Addgene viral prep # 55632-AAVrg). pAAV-CAG- FLEX-rc [Jaws-KGC-GFP-...Jaws-KGC-GFP-ER2] was a gift from Edward Boyden (Addgene viral prep # 84445-AAVrg).   Electron Microscopy The ratio...Scale bar = 100 nm.   AAV resources Browse AAV preps available from Addgene. After receiving your virus....   Lentivirus resources Browse lentiviral preps available from Addgene. After receiving your virus...

...to provide ready-to-use viral preps. Here are some of the new viral preps from recent months: Second generation...highlight a subset of the new plasmids and viral preps in the repository through our hot plasmids articles... cells The CRISPR Corner New ready-to-use viral preps   Improved cell-free transcription/translation ... Dimidschstein Lab More GCaMP8 ready-to-use AAV preps  ...

...to provide ready-to-use viral preps. Here are some of the new viral preps from recent months: Our selection...highlight a subset of the new plasmids and viral preps in the repository through our hot plasmids articles...strigolactones The CRISPR Corner New ready-to-use viral preps New toolkit for CRISPR-based genome editing in...selection of GCaMP8 AAV preps has expanded. Check out the new selection of ready-to-use AAV9 and AAVrg vectors...