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We narrowed to 13 results for: PREP

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Showing: 1 - 13 of 13 results

  2. Protocol - How to Create a Bacterial Glycerol Stock

    Type
    Protocol
    ...able to start an overnight culture for plasmid DNA prep the following day. Tips and FAQ The optimal concentration...glycerol stock before you begin your plasmid mini-prep. Try not to freeze/thaw your glycerol stock too ...

  3. AAV Purification by Iodixanol Gradient Ultracentrifugation

    Type
    Protocol
    ...ultracentrifugation to get pure adeno-associated virus (AAV) preps for use in your research....distinguish the steps. This method will help enrich the prep with full (genome containing) particles. On average...Ultracentrifuge T70i rotor or equivalent Reagents Opti-Prep (60% Iodixanol) QuickSeal tubes QuickSeal tube spacers...

  4. Fluorescence Titering Assay

    Type
    Protocol
    ...polyethersulfone filter, Nalgene, 565-0010 (for viral preps, if prep was not previously filtered) Microcentrifuge...

  5. Colony Formation Titering Assay

    Type
    Protocol
    ...polyethersulfone filter, Nalgene, 565-0010 (for viral preps, if prep was not previously filtered) Microcentrifuge...

  6. DNA Quantification

    Type
    Protocol
    ...NanoDrop to measure your samples, place 1-2µL of mini-prepped DNA onto the pedestal. Close the lid and click ...

  8. Handling Plasmids from Addgene - Purifying Plasmid DNA

    Type
    Protocol
    ...you can find a protocol for kit-free plasmid mini-prep at the bottom of this page. Last Update: Feb. 8,...culture of bacteria . Pro-Tip Refer to appropriate DNA prep protocol for volume of bacteria to grow (low copy...

  9. Coomassie Purity Stain of Recombinant Antibodies

    Type
    Protocol
    ...appropriate imaging system. Figure 1 Recombinant antibody preps should have 2 clear bands at ~50 kDa and ~25 kDa... Pro-Tip Addgene only uses recombinant antibody preps that have a purity of ≥90% but some labs use unpurified...

  10. AAV Titration by qPCR Using SYBR Green Technology

    Type
    Protocol
    ... number of genome-containing particles in an AAV prep using qPCR, SYBR Green Technology, and Addgene’s... number of genome-containing particles of an AAV prep using SYBR green technology. This protocol was validated...

  13. AAV Production in HEK293 Cells

    Type
    Protocol
    ... °C for 45 min. You are now ready to purify your prep . The clarified supernatant can be kept overnight...
Showing: 1 - 13 of 13 results