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Plasmid#46559PurposeMammalian expression plasmid with EGFP expressed from CMV wild-type promoter.DepositorInsertCMV wt promoter
UseSynthetic BiologyTagsExpressionMammalianMutationOur version has a G at base 724 (reference has C)PromoterCMVAvailable sinceSept. 20, 2013AvailabilityAcademic Institutions and Nonprofits only -
aMHC-puro rex-neo
Plasmid#21230DepositorInsertalpha myosin heavy chain promoter
UseLentiviralTagsExpressionMutationPromoterAvailable sinceSept. 30, 2009AvailabilityAcademic Institutions and Nonprofits only -
NST1_pOpOn2.1
Plasmid#228065PurposeExpresses NST1 in different cell types in Arabidopsis thaliana upon induction with dexamethasone.DepositorInsertNAC SECONDARY WALL THICKENING PROMOTING FACTOR1, NST1 (NST1 Mustard Weed)
UseDexamethasone inducible vectorTagsExpressionPlantMutationPromoterCaMV35S for LhGR and pOp6-minimal CaMV35S for NST1Available sinceJan. 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
natMX6-ins5
Plasmid#195042PurposepFA6a derived selection cassette flanked with transcription terminators (tDEG1/tCYC1), allows genome modification without disruption of insertion neighboring genes by transcription interferenceDepositorInsertNatR
UseYeast genomic targetingTagsS. cerevisiae DEG1 terminator, A. gossypii TEF pr…ExpressionMutationPromoterAvailable sinceFeb. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-myc-PDGFRA-R914W_EGFP-PEST_reporter
Plasmid#172596PurposeMammalian fluorescent (EGFP-PEST) reporter plasmid for PDGFRA-R914W mutant signaling.DepositorInsertmyc-PDGFRA-R914W (PDGFRA Human, Synthetic)
UseEgfp-pest_reporterTagsExtracellular c-myc epitope tag and Signal peptid…ExpressionMammalianMutationPDGFRA-R914WPromoterCMVAvailable sinceOct. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-myc-PDGFRA-D842V_EGFP-PEST_reporter
Plasmid#172598PurposeMammalian fluorescent (EGFP-PEST) reporter plasmid for PDGFRA-D842V mutant signaling.DepositorInsertmyc-PDGFRA-D842V (PDGFRA Human, Synthetic)
UseEgfp-pest_reporterTagsExtracellular c-myc epitope tag and Signal peptid…ExpressionMammalianMutationPDGFRA-D842VPromoterCMVAvailable sinceOct. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-myc-PDGFRA-K627R_EGFP-PEST_reporter
Plasmid#172597PurposeMammalian fluorescent (EGFP-PEST) reporter plasmid for PDGFRA-K627R mutant signaling.DepositorInsertmyc-PDGFRA-K627R (PDGFRA Human, Synthetic)
UseEgfp-pest_reporterTagsExtracellular c-myc epitope tag and Signal peptid…ExpressionMammalianMutationPDGFRA-K627RPromoterCMVAvailable sinceOct. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
GS2.1/EC
Plasmid#132568PurposesgRNA targeting A. thaliana pds3, regulated by A. thaliana U6 polIII promoter. Cas9 with a C-terminal mApple fusion, egg cell-specific expression. Hygromycin selectable marker (hpt).DepositorInsertegg cell promoter, Cas9-mApple, pds3 sgRNA
UseCRISPR and Synthetic BiologyTagsExpressionPlantMutationPromoterA. thaliana egg cell promoterAvailable sinceDec. 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET28a-LgBiT-SpyCatcher
Plasmid#223634PurposeProtein purification of SpyCatcher003 (S49C) with LgBiT and 6xHis tags from E. coliDepositorInsertSpyCatcher003 S49C
UseTagsHis6, LgBiTExpressionBacterialMutationPromoterAvailable sinceSept. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a-SpyCatcher -AVI
Plasmid#223635PurposeProtein purification of SpyCatcher003 (S49C) with AVI and 6xHis tags from E. coliDepositorInsertSpyCatcher003 S49C
UseTagsAVI tag and His6ExpressionBacterialMutationPromoterAvailable sinceSept. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
CAGGS-safb∆sap-NLS-3XFLAG-V5
Plasmid#196091PurposeExpresses mouse SAFB lacking the n-terminal SAP domain in mammalian cells. Contains a c-terminal 3xFlag tag and a c-terminal V5 tagDepositorInsertSAFB (Safb Mouse)
UseTagsC-terminal 3xFLAG and C-terminal V5ExpressionBacterial and MammalianMutationRemoved amino acids 31-65 (SAP domain) of mouse S…PromoterCAGGSAvailable sinceSept. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
CAGGS-safb∆rrm-NLS-3XFLAG-V5
Plasmid#196093PurposeExpresses mouse SAFB lacking the rrm domain in mammalian cells. Contains a c-terminal 3xFlag tag and a c-terminal V5 tagDepositorInsertSAFB (Safb Mouse)
UseTagsC-terminal 3xFLAG and C-terminal V5ExpressionBacterial and MammalianMutationRemoved amino acids 428-506 (RRM) of mouse SAFB p…PromoterCAGGSAvailable sinceSept. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
CAGGS-safb∆dd1-NLS-3XFLAG-V5
Plasmid#196092PurposeExpresses mouse SAFB lacking the first computationally called disordered domain in mammalian cells. Contains a c-terminal 3xFlag tag and a c-terminal V5 tagDepositorInsertSAFB (Safb Mouse)
UseTagsC-terminal 3xFLAG and C-terminal V5ExpressionBacterial and MammalianMutationRemoved amino acids 96-285 (predicted disordered …PromoterCAGGSAvailable sinceSept. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
CAGGS-safb∆dd3-NLS-3XFLAG-V5
Plasmid#196094PurposeExpresses mouse SAFB lacking the third computationally called disordered domain in mammalian cells. Contains a c-terminal 3xFlag tag and a c-terminal V5 tagDepositorInsertSAFB (Safb Mouse)
UseTagsC-terminal 3xFLAG and C-terminal V5ExpressionBacterial and MammalianMutationRemoved amino acids 638-925 (predicted disordered…PromoterCAGGSAvailable sinceSept. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
CAGGS-SAFB-DD3-NLS-3XFLAG-V5
Plasmid#196095PurposeExpresses only the third computationally called disordered domain of mouse SAFB in mammalian cells. Contains a c-terminal 3xFlag tag and a c-terminal V5 tagDepositorInsertSAFB (Safb Mouse)
UseTagsC-terminal 3xFLAG and C-terminal V5ExpressionBacterial and MammalianMutationContains only AA 619-925 (NLS and predicted disor…PromoterCAGGSAvailable sinceSept. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pProm1_BCD1-GFP
Plasmid#80649PurposePromoter1 with BCD1-gfpDepositorInsertGFP
UseP15a origin, constitutive promoter, kanrTagsExpressionMutationNONEPromoterconstitutive promoterAvailable sinceSept. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pProm11_BCD1-GFP
Plasmid#80656PurposePromoter11 with BCD1-gfpDepositorInsertGFP
UseP15a origin, constitutive promoter, kanrTagsExpressionMutationNONEPromoterconstitutive promoterAvailable sinceAug. 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
pProm9_BCD1-GFP
Plasmid#80655PurposePromoter9 with BCD1-gfpDepositorInsertGFP
UseP15a origin, constitutive promoter, kanrTagsExpressionMutationNONEPromoterconstitutive promoterAvailable sinceAug. 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
pProm1_BCD20-GFP
Plasmid#80651PurposePromoter1 with BCD20-gfpDepositorInsertGFP
UseP15a origin, constitutive promoter, kanrTagsExpressionMutationNONEPromoterconstitutive promoterAvailable sinceAug. 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
pProm1_BCD21-GFP
Plasmid#80652PurposePromoter1 with BCD21-gfpDepositorInsertGFP
UseP15a origin, constitutive promoter, kanrTagsExpressionMutationNONEPromoterconstitutive promoterAvailable sinceAug. 3, 2016AvailabilityAcademic Institutions and Nonprofits only