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We narrowed to 62 results for: c-Myc-promoter

Showing: 1 - 20 of 62 results

  1. pCXLE toolkit: Efficient episomal plasmid-based method to reprogram peripheral blood cells to iPSCs

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    Blog Post
    ...enhances iPSC generation as does replacing C-MYC with L-MYC [10, 11]. While the mechanisms underlying ...addition of an shRNA for P53 and replacing C-MYC with L-MYC in the episomal reprogramming cocktail by ...Nakagawa, M., et al., Promotion of direct reprogramming by transformation-deficient Myc. Proc Natl Acad Sci... reprogramming factors- OCT3/4, SOX2, KLF4, and C-MYC [1]. For the derivation of clinical grade hiPSCs... that encoded six factors - OCT3/4, SOX2, KLF4, C-MYC, LIN28 and SV40 large T antigen (SV40LT). The method...the site of insertion and which can potentially promote tumorigenesis in vivo. The phenomenon has been ...pCXLE-hSK 27078 SOX2 and KLF4 pCXLE-hUL 27080 L-MYC and LIN28 pCXLE-hOCT3/4-shp53-F 27077 OCT3/4 and...

  2. Hot Plasmids and Viral Preps - September 2021

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    Blog Post
    ...genome-wide. It was designed based on MYC-ChIP-seq data from several MYC-dependent cancer cell lines. It can...selection marker, Thy1.1, in addition to puromycin resistance.  The MYC-CRISPR library targets E-boxes genome-wide...optimized their initial system by using a stronger promoter and different biochemical secondary energy sources... can be applied for identification of essential MYC binding sites in a wide range of human cells.  A new... system is capable of robust expression of high G+C content genes, as validated through monitoring the...’s lab previously developed such a system in Streptomyces, a prokaryotic genus capable of producing a ...

  3. Delivery Methods for Generating iPSCs

    Type
    Blog Post
    ...Zhi Ying Chen, Robert C. Robbins, Mark A. Kay, Michael T. Longaker, and Joseph C. Wu. "A nonviral minicircle... viral particles that express oncogenes, such as Myc. Viral vectors also have the largest genomic footprint...transgenes is a concern as well, and reactivation of Myc or other oncogenes after differentiation has been...expresses multiple reprogramming factors driven by one promoter. While lentivirus has similar rates of reprogramming...control. Additionally, Sendai virus doesn’t rely on promoters for transcriptional regulation, so it requires... mini-plasmids. They contain only a eukaryotic promoter and the cDNA(s) to be expressed and they don’t...phosphatase, expression of embryonic proteins, and promoter demethylation and expression of endogenous pluripotency...

  4. Fluorescent Tagging of Endogenous Genes with SapTrap

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    Blog Post
    ...gRNA to the U6 promoter, and a second plasmid containing the homology arms and an N- or C-terminal tag.... site accepts the sgRNA target sequence for U6 promoter expression and the second site accepts the homology... T2A-TurboGFP-PEST), and small epitope tags (HA, Myc, Strep tag II, AviTag, HaloTag, SpyTag). The Foerstemann...regions of homology to the gene of interest to promote homology directed repair after a CRISPR/Cas9 induced...PCR or by evaluating visual phenotypes. Improving C. elegans fluorescent protein tagging with SapTrap ...called SapTrap to improve CRISPR genomic tagging in C. elegans. SapTrap offers scientists a convenient one...protein tagging systems For those who do not work with C. elegans, several groups have designed modular toolkits...

  5. Plasmids 101: Protein tags

    Type
    Blog Post
    ...to it. The FLAG, hemaglutinin antigen (HA), and c-myc tags have been the workhorses of the affinity tag...step purification with amylose, but wicked huge Myc EQKLISEEDL  1.2 Affinity Frequently used for western...amino acid protein  12 Stability At N-terminus, promotes folding and structural integrity; cleavable. Not... tags, a His tag can be fused to either the N- or C-terminus of a protein. Unlike other epitope tags –...

  6. Hot Plasmids: Spring 2025

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    Blog Post
    ...new and known regulators at the FOS gene promoter and the MYC locus (Figure 5). Figure 5: TurboCas...new backbones special: Strong expression: A CMV promoter drives robust gene expression in mammalian cells...or add a versatile N- or C-terminal HA tag. Selectable and stable: A puromycin resistance gene makes stable...creation a breeze. Modular by design: Key elements (promoter, tag, polyA site) are flanked by unique restriction...identification of heat-shock proteins at the FOS gene promoter using TurboCas, streptavidin immunoprecipitation...expressing iGluSnFR3, iGluSnFR4f, and iGluSnFR4s. C) Spike-triggered averages for single action potential-evoked...integrase-based approaches. Find STITCHR plasmids here! Fell, C. W., Villiger, L., Lim, J., Hiraizumi, M., … Abudayyeh...

  8. Empty Backbones - Choosing Your Perfect Plasmid Backbone

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    Collection
    ...display vector with a C-terminal Myc tag pPMW-attB - pUASp plasmid with N-terminal Myc tag and attB for Drosophila...N-terminal Myc tag for mammalian expression pGEX-4T-1-3xMyc - Bacterial vector for Myc tag pETcon(-) - Yeast...plasmid with a promoter that will be functional in your host organism. Host Relevant Promoters Representative...vector for C-terminal 3x HA tag fusion with your gene of interest in plants Myc Epitope tag pKMyc - N-terminal...transgenesis His Epitope tag pEZYmyc-His - C-terminal Myc-His tag for mammalian expression...interest to create a luciferase reporter Promoter Measure promoter strength pBV-Luc - Luciferase ...Gileadi lab Worm unc-54, variety of worm gene promoters C. elegans vector kit - Collection...

  9. 28 Hot Plasmid Technologies from 2015

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    Blog Post
    ...fluorescent proteins, 8 constitutive promoters, 2 includible promoters, 3 polyA terminators and various pieces...sequences. Several pre-constructed promoter-selection marker or promoter-inducible expression related vectors...all chemically inducible promoters; 3) improving the strength of the promoters used; and 4) optimizing ...ideal promoter/location combination. However, other constructs which contain different promoters and/or...to study the human GM-CSF promoter and enhancer, a finely regulated promoter controlled by a mixture of... Cas9 into n-terminal (Cas9(N)-2xNLS) and c-terminal (Cas9(C)-2xNLS) fragments facilitates target DNA ...activation, the c-terminal cas9 fragment was fused with FK 506 binding protein 12 (Cas9(C)-FKBP-2xNLS) and...

  10. 15 Hot Plasmids from 2017

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    Blog Post
    ...includes four promoters, six fluorophores with nonoverlapping emission spectra (N- and C-terminal tags...of transcription factors OCT3/4, SOX2, KLF4, and c-MYC is the established model for reprogramming somatic...neurodegenerative diseases, the Cole Lab selected promoters for cell types directly linked to disease, including...article have been deposited with Addgene and include MYC- and FLAG-tagged TZAP constructs (wild-type and truncation... components can be controlled by a variety of promoters for versatile and robust engineering. These components...coselection, analogous to methods previously used in C. elegans, targets the sodium/potassium pump ATP1A1... the B. subtilis Single Gene Deletion Library—Kanamycin and the CRISPRi Essential gene Knockdown library...

  11. Starter Guide to induced Pluripotent Stem Cells (iPSCs) Part 2:  Reprogramming and Transdifferentiation

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    Blog Post
    ...four reprogramming factors- OCT4, SOX2, KLF4, and C-MYC - that induce a differentiated somatic cell to revert...additional pluripotency stage) Less Safety Less (C-MYC, a known oncogene is one of the reprogramming factors...iPSC-derived osteoblasts and osteoclasts together promote bone regeneration in 3D biomaterials. Sci Rep, ...22645348. PubMed Central PMCID: PMC3390875. 25. Patsch, C., et al., Generation of vascular endothelial and smooth... PubMed Central PMCID: PMC4363833. 35. Eguizabal, C., et al., Dedifferentiation, transdifferentiation,...1): p. 82-94. PubMed PMID: 23329641. 36. Jopling, C., S. Boue, and J.C. Izpisua Belmonte, Dedifferentiation...

  12. Plasmids for Stem Cell Research

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    ...Klf4, Myc, and Brd3R from five separate lentiviral plasmids The acetyllysine reader BRD3R promotes human...state using a cocktail of factors (Oct3/4, Sox2, c-Myc, and Klf4) that are known to maintain pluripotency...Doxycycline-inducible expression of human Oct4, Sox2, Klf4, and c-Myc from four separate lentiviral plasmids A drug-inducible...for the expression of human Oct4, Klf4, Sox2, and c-Myc Human Induced Pluripotent Stem Cells Produced Under... vector for the expression of human Oct4, Klf4, c-Myc, and Sox2 Integrative Analyses of Human Reprogramming...Lentivirus Human Expression of human Klf4, Oct4, c-Myc, and Sox2 as VP-16 transcriptional activating fusions...polycistronic expression of human Oct4, Klf4, Sox2, c-Myc and hairpin RNA p53 for single plasmid reprogramming...

  13. Hassle-free 96-well Format Epitope Tagging Using Cas9 Ribonucleoprotein

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    Blog Post
    ...(3xFLAG, Myc, V5, or HA). Tag-specific PCR genotyping can tell if HDR completely failed. If C-terminus...Kalantzaki M, Gangoso E, Matjusaitis M, Garcia-Diaz C, Blin C, Grant V, Bulstrode H, Gogolok S et al. 2017. ...will be suitable). For N-terminus tagging, the promoter region of both the alleles should be sequence-...cautiously analyzed for any damage (indels) to the promoter region of the non-HDR allele. For N-terminal tagging...available for human and mouse genomes only and deals with C-terminus epitope tagging. If you work with other model...your crRNAs using other web-based design tools (for C-terminus tagging, pick a crRNA that cuts 8-15 bp downstream...insertion site (within ~8-15 bp from the stop codon for C-terminus tagging) GC content of the crRNA (aim for...

  14. Prime Editing: Adding Precision and Flexibility to CRISPR Editing

    Type
    Blog Post
    ...j.cell.2021.09.018 Choi, J., Chen, W., Suiter, C. C., Lee, C., Chardon, F. M., Yang, W., Leith, A., Daza,...expression ~ ~ ~ ~ ✓   ✓ SV40 and c-Myc nuclear localization sequences Improve translocation..., J., Oyler-Castrillo, P., Ravisankar, P., Ward, C. C., Levesque, S., Jing, Y., Simpson, D., Zhao, A.,...complex, to temporarily inhibit this process and promote copying of the edited strand to the unedited strand... co-express mismatch repair inhibitor MLH1dn Promote desired resolution of heteroduplex     ✓ ...Leukemia Virus (M-MLV) reverse transcriptase to the C-terminus of Cas9 H840A nickase (PE1). But innovations...pegRNAs (Yan et al., 2024). The team fused La to the C-terminal end of PEmax to produce PE7. PE7 significantly...

  15. Luciferase Plasmid Collection

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    Collection
    ...of 5' promoter/enhancer regions Joshua Mendell 60323 pGL4.23-GW Firefly Insertion of 5' promoter/enhancer...five transcriptional reporters for NF-kb, TGF-b, c-Myc, p53, and MAPK/JNK plus a constitutive control. ...tool for scientists. Regulatory elements such as promoters, enhancers and untranslated regions, or shRNA ...Jorge Ferrer 16539 pBV-Luc Firefly Insertion of 5' promoter/enhancer regions Bert Vogelstein 12178 pIS0 Firefly... Renilla luciferase under the control of a CMV promoter is present for normalization William Kaelin 71248...71248 pXPG Firefly Insertion of 5' promoter/enhancer regions. Mutations within the luciferase gene allow... Renilla Dual reporter vector. Insertion of 5' promoter/enhancer regions upstream of Firefly luciferase...

  16. 27 Hot Plasmids from 2016

    Type
    Blog Post
    ... 79628 (C) Cv (capsid) N and C 79633 (N) and 79641 (C) NS1 (var: W98G) N and C 79635 NS3 C 79637...79637 NS2B C 79636 NS4A N 79640 NS4B C 79639 NS5 C 79632 (N) and 79631 (C) PrM N and C   Detecting... gRNA from a Drosophila U6:2 promoter and Cas9 from the actin 5C promoter. Addgene’s fly library web page... over 30 promoters and 140 genes, including constructs with unique tissue-specific promoters expressing...tagged BioID2 for N-terminal fusions and Myc tagged BioID2 for C-terminal fusions. Kim, et al. Mol Biol...genomic libraries are cloned downstream of a minimal promoter sequence. Should one of these random stretches...activate its own transcription from the minimal promoter and its strength can be determined from its enrichment...

  17. Even more elegant: Single injection CRISPR/Cas9 in C. elegans

    Type
    Blog Post
    ...targeted gene from its promoter, creating a loss-of-function allele as well as a promoter-FP fusion for the...genome engineering methods for C. elegans were released, signaling a new era in C. elegans research. Homology...of custom genomic modifications, is very robust in C. elegans, and the methods for HDR-mediated modification...the University of North Carolina has made CRISPR in C. elegans even easier - now, one can generate a fluorescent...-based gene tagging approach Multiple features of C. elegans have made CRISPR/Cas9 so successful in this...previously used a transgene selection strategy for their C. elegans/CRISPR experiments, but this strategy required... make it possible fluorescently label proteins in C. elegans, they inserted the SEC into a synthetic intron...

  18. Plasmids 101: Repressible Promoters

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    Blog Post
    ... are repressible promoters regulated? Like inducible promoters, repressible promoters can be regulated...Promoters control the binding of RNA polymerase and transcription factors. Since the promoter region ...natural and engineered repressible promoters. Chemically repressible promoters Tet-Off In its native context... pADH1 displays low promoter activity (~20% activity of the strong yeast promoter pTEF). As ethanol accumulates...that promoter is active. In this way, you can interrogate the activity of uncharacterized promoters. Similarly... QUAS promoter, and the QF transcription factor cannot drive transcription from the UAS promoter. This...repressible promoter systems, but there are certainly more out there! The repressible promoter systems detailed...

  19. CRISPR Methods for Bacteria: Genome Engineering, CRISPRa, CRISPRi, Base Editing, and More

    Type
    Blog Post
    ...dependent promoters, Baojun Wang’s lab developed a system that can activate sigma-54 dependent promoters (Liu...activation of sigma-54 type promoters. The system was functional in activating promoters in Pseudomonas syringae...that utilizes phage recombination machinery to promote homologous recombination of linear DNA fragments...regulate gene expression were limited to inducible promoters or direct gene knockout. In contrast, CRISPR offers...systems, catalytically dead (dCas9) targeted to a promoter or gene body can repress transcription by physically...and can be more effective targeting either the promoter or the coding sequence and the template or non-template...to multiplex repression. Using a Tet responsive promoter to drive expression of a catalytically dead Cas9...

  20. Tetracycline Inducible Expression

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    Collection
    ...may also like... Blog: Inducible Promoters Blog: Repressible Promoters Mammalian shRNA Plasmids Collection...upstream of a minimal CMV promoter, and other tet- or dox-dependent promoters (sometimes generally called... CMV promoter. In the absence of tetracycline, tTA binds to the TRE and its VP16 domain promotes gene ...transactivators and promoters are generally cross-compatible. Your choice of transactivator, promoter, and type ... express Tet-On 3G transactivator under the c-Fos promoter Tet-On 3G rtTA Bong-Kiun Kaang 20342 FUW-M2rtTA...opens in a new window) Krueger, C., Pfleiderer, K., Hillen, W., & Berens, C. (2004). Tetracycline derivatives...Considerations Tet Plasmids Inducible and repressible promoters are powerful tools to control gene expression ...
Showing: 1 - 20 of 62 results