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mRFP-Ub

Plasmid

#11935

Depositor

Nico Dantuma

Article

Bergink et al Genes Dev. 2006 May 15. 20(10):1343-52.

Insert

Ubiquitin (RBX1 Human)

Tags

mRFP

Expression

Mammalian

Available Since

July 5, 2006

Availability

Academic Institutions and Nonprofits only

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AAV-hSyn-Soma-jGCaMP8m

Plasmid

#169257

Purpose

AAV transfer plasmid for neuronal expression of soma-targeted jGCaMP8m.

Depositor

Marianne Fyhn

Article

Grodem et al Nat Commun. 2023 Feb 4;14(1):608. doi: 1

Has Service

AAV Retrograde, AAV1, and AAV9

Insert

Soma-jGCaMP8m

Use

AAV

Tags

6xHis-tag

Expression

Mammalian

Available Since

May 18, 2021

Availability

Academic Institutions and Nonprofits only

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tdTomato-H2B-6

Plasmid

#58101

Purpose

Localization: Nucleus/Histones, Excitation: 554, Emission: 581

Depositor

Michael Davidson

Article

Planchon et al Nat Methods. 2011 May;8(5):417-23. doi:

Insert

H2B (H2BC11 Human)

Tags

tdTomato

Expression

Mammalian

Mutation

D26G and V119I in H2B

Promoter

CMV

Available Since

Dec. 23, 2014

Availability

Academic Institutions and Nonprofits only

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pGEX6P1-mCherry-anti-mouse-IgG(kappa)-nanobody

Plasmid

#138130

Purpose

Expresses GST-tagged mCherry-anti-mouse-IgG(kappa)-nanobody in E. coli cells.

Depositor

Kazuhisa Nakayama

Article

Katoh et al Mol Biol Cell. 2020 Jul 29:mbcE20040250.

Insert

mCherry-anti-mouse-IgG(kappa)-nanobody

Tags

GST

Expression

Bacterial

Available Since

July 30, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-synapsin-WHaloCaMP1a

Plasmid

#205308

Purpose

Production of AAV particles encoding WHaloCaMP1a from the synapsin promoter

Depositor

Eric Schreiter

Article

Farrants et al bioRxiv. 2023 Jul 19:2023.07.18.549527.

Insert

WHaloCaMP1a

Use

AAV

Available Since

Aug. 18, 2023

Availability

Academic Institutions and Nonprofits only

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lenti F9-TetR-EGFP-IRES-PuroR

Plasmid

#117049

Purpose

lentiviral vector for expression of TetR-EGFP, for visualization of TetO repeats

Depositor

Huimin Zhao

Article

Tasan et al Nucleic Acids Res. 2018 Jun 15. pii: 503

Insert

F9-TetR-EGFP

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Promoter

F9 promoter

Available Since

March 7, 2019

Availability

Academic Institutions and Nonprofits only

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pmScarlet_alphaTubulin_C1

Plasmid

#85045

Purpose

In vivo visualization of microtubules (can be used for colocalization studies)

Depositor

Dorus Gadella

Article

Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

Insert

Alpha-Tubulin (TUBA1B Human)

Tags

mScarlet

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pGEX6P1-EGFP-anti-mouse-IgG(kappa)-nanobody

Plasmid

#138132

Purpose

Expresses GST-tagged EGFP-anti-mouse-IgG(kappa)-nanobody in E. coli cells.

Depositor

Kazuhisa Nakayama

Article

Katoh et al Mol Biol Cell. 2020 Jul 29:mbcE20040250.

Insert

EGFP-anti-mouse-IgG(kappa)-nanobody

Tags

GST

Expression

Bacterial

Available Since

July 30, 2020

Availability

Academic Institutions and Nonprofits only

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pCAG-WHaloCaMP1a-EGFP

Plasmid

#205313

Purpose

Expression of WHaloCaMP1a-EGFP in mammalian cells from the CAG promoter

Depositor

Eric Schreiter

Article

Farrants et al bioRxiv. 2023 Jul 19:2023.07.18.549527.

Insert

WHaloCaMP1a-EGFP

Expression

Mammalian

Available Since

Aug. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pQE-60 Grx1-roGFP2-His

Plasmid

#64799

Purpose

Bacterial expression of His-tagged Grx1-roGFP2

Depositor

Tobias Dick

Article

Gutscher et al Nat Methods. 2008 Jun;5(6):553-9. doi: 1

Insert

Glutaredoxin-1 (GLRX Human)

Tags

His and roGFP2

Expression

Bacterial

Available Since

Aug. 19, 2015

Availability

Academic Institutions and Nonprofits only

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tetO-PBX1

Plasmid

#170699

Purpose

doxycycline-inducible overexpression of PBX1

Depositor

Paul Burridge

Article

Romero-Tejeda et al J Mol Cell Cardiol. 2023 Jul 6:S0022-282

Insert

PBX1 (PBX1 Human)

Use

Lentiviral; Doxycycline inducible

Expression

Mammalian

Promoter

TRE promoter, Tet-ON

Available Since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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CMV-GEM-GECO1

Plasmid

#32442

Purpose

Mammalian expression of blue-green emission ratiometric genetically encoded Ca2+ indicator for optical imaging

Depositor

Robert Campbell

Article

Zhao et al Science. 2011 Sep 30;333(6051):1888-91.

Insert

GEM-GECO1

Expression

Mammalian

Mutation

GCaMP3 L60P/K69E/N77Y/D86G/N98I/K119I/L173Q/T223S…

Promoter

CMV

Available Since

Sept. 29, 2011

Availability

Academic Institutions and Nonprofits only

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145-pGL3ctrl-3' UTR

Plasmid

#28175

Depositor

Michael Kastan

Article

Chen et al Genes Dev. 2010 Oct 1. 24(19):2146-56.

Insert

Luciferase flanked by tp53 UTRs (TP53 Human)

Use

Luciferase

Expression

Mammalian

Mutation

Note that insert length is based on sum of the 2 …

Available Since

March 15, 2011

Availability

Academic Institutions and Nonprofits only
Tg(mpeg1:LifeAct-mRuby)

Plasmid

#166977

Purpose

labels F-actin in macrophages

Depositor

Anna Huttenlocher

Article

Barros-Becker et al J Cell Sci. 2017 Nov 15;130(22):3801-380

Inserts

mpeg1 (mpeg1.1 Zebrafish)
LifeAct (actb1 Frog)

Tags

Lifeact-mRuby and mRuby

Available Since

Oct. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-HA-Flag-natT1R2

Plasmid

#113944

Purpose

mammalian expression plasmid for FLAG-tagged human T1R2 with signal peptide of influenza hemagglutinin

Depositor

Erik Procko

Article

Park et al J Biol Chem. 2019 Feb 5. pii: RA118.0061

Insert

T1R2 (TAS1R2 Human)

Tags

FLAG and Signal/leader sequence from influenza he…

Expression

Mammalian

Mutation

residues 22-839

Available Since

Feb. 8, 2019

Availability

Academic Institutions and Nonprofits only

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GFP-Ub KO

Plasmid

#11934

Depositor

Nico Dantuma

Article

Bergink et al Genes Dev. 2006 May 15. 20(10):1343-52.

Insert

Ubiquitin KO (RBX1 Human)

Tags

GFP

Expression

Mammalian

Mutation

All 7 lysines of ubiquitin mutated to arginines.

Available Since

Aug. 7, 2006

Availability

Academic Institutions and Nonprofits only

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pSW002-Pc-mNeonGreen

Plasmid

#205016

Purpose

Broad host-range bacterial expression vector with constitutive Pc promoter; for tagging bacteria with mNeonGreen (codon optimized for P. fluorescens)

Depositor

Rosemarie Wilton

Article

Wilton et al Front Plant Sci. 2018 Feb 1;8:2242. doi:

Insert

mNeonGreen

Expression

Bacterial

Mutation

mNeonGreen is codon optimized for expression in P…

Promoter

Pc

Available Since

Feb. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pCAG-G-Flamp1-mut

Plasmid

#188568

Purpose

control probe of G-Flamp1, insensitive to cAMP

Depositor

Jun Chu

Article

Wang et al Nat Commun. 2022 Sep 12;13(1):5363. doi:

Insert

G-Flamp1-mut

Tags

His tag

Expression

Mammalian

Promoter

CAG

Available Since

Nov. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-dLight1.5

Plasmid

#111058

Purpose

Expresses dLight1.5 in mammalian cells

Depositor

Lin Tian

Article

Patriarchi et al Science. 2018 Jun 29;360(6396). pii: sci

Insert

dLight1.5

Tags

Flag tag

Expression

Mammalian

Available Since

July 23, 2019

Availability

Academic Institutions and Nonprofits only

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VAMP2-pHuji

Plasmid

#105289

Purpose

VAMP2 tagged with pH-sensitive red fluorescent protein pHuji

Depositor

Justin Taraska

Article

Martineau et al Nat Commun. 2017 Nov 10;8(1):1412. doi:

Insert

VAMP2 (Vamp2 Rat)

Tags

pHuji

Expression

Mammalian

Available Since

May 22, 2018

Availability

Academic Institutions and Nonprofits only

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