We narrowed to 20,061 results for: nans

1. pVITRO1-102.1F10-IgG2/λ

   Plasmid

   #50367

   Purpose

   Expresses grass pollen allergen Phl p 7 specific human IgG2/λ antibody isotype (102.1F10-IgG2/λ)

   Depositor

   Andrew Beavil

   Article

   Dodev et al Sci Rep. 2014 Jul 30;4:5885. doi: 10.103

   Inserts

   grass pollen allergen Phl p 7 specific human Gamma 2 heavy chain expression cassette
   grass pollen allergen Phl p 7 specific human lambda light chain expression cassette
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Mouse Elongation Factor 1 Alpha and Rat Elongatio…

   Available since

   Jan. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pVITRO1-102.1F10-IgG3/λ

   Plasmid

   #50368

   Purpose

   Expresses grass pollen allergen Phl p 7 specific human IgG3/λ antibody isotype (102.1F10-IgG3/λ)

   Depositor

   Andrew Beavil

   Article

   Dodev et al Sci Rep. 2014 Jul 30;4:5885. doi: 10.103

   Inserts

   grass pollen allergen Phl p 7 specific human Gamma 3 heavy chain expression cassette
   grass pollen allergen Phl p 7 specific human lambda light chain expression cassette
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Mouse Elongation Factor 1 Alpha and Rat Elongatio…

   Available since

   Jan. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-hSyn Con/Foff EYFP

   Plasmid

   #55651

   Purpose

   Cre-on/Flp-off EYFP under the Synapsin promoter

   Depositor

   Karl Deisseroth

   Article

   Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

   Insert

   eYFP with introns
   

   Use

   AAV; Cre on/flp off eyfp

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hSyn

   Available since

   Aug. 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-Ef1a-DIO hChR2(E123T/T159C)-EYFP

   Plasmid

   #35509

   Purpose

   AAV expression of EF1a-driven, Cre-dependent, humanized channelrhodopsin E123T/T159C mutant fused to EYFP for optogenetic activation.

   Depositor

   Karl Deisseroth

   Article

   Mattis et al Nat Methods. 2011 Dec 18;9(2):159-72. do

   Has Service

   AAV5 and AAV9

   Insert

   hChR2
   

   Use

   AAV

   Tags

   EYFP

   Expression

   Mammalian

   Mutation

   E123T and T159C

   Promoter

   Ef1a

   Available since

   April 18, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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5. TagBFP2-INPP4BC842A-CAAX

   Plasmid

   #116858

   Purpose

   inactive plasma membrane targetted PI(3,4)P2 phosphatase

   Depositor

   Gerry Hammond

   Article

   Goulden et al J Cell Biol. 2019 Mar 4;218(3):1066-1079

   Insert

   INPP4B (INPP4B Human)
   

   Use

   Tags

   HRAS(172-189) and TagBFP2

   Expression

   Mammalian

   Mutation

   catalytic cysteine 842 changed to alanine

   Promoter

   Available since

   Oct. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pQE80L-SpyCatcher-ELP-GFP

   Plasmid

   #69835

   Purpose

   Bacterial expression plasmid containing SpyCatcher-ELP-GFP fusion. SpyCatcher forms a covalent bond with SpyTag and can be used to label plasma membrane localized SpyTag-C1C2 in live cells.

   Depositor

   Viviana Gradinaru

   Article

   Bedbrook et al Chem Biol. 2015 Aug 20;22(8):1108-21. do

   Insert

   SpyCatcher-ELP-GFP
   

   Use

   Tags

   6x His Tag, GFP, and TEV Tag

   Expression

   Bacterial

   Mutation

   Promoter

   T5 promoter/lac operator element

   Available since

   July 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBabe RFP1-KRT5 hygro

   Plasmid

   #58493

   Purpose

   mammalian expression of mRFP1-tagged human KRT5

   Depositor

   Kevin Janes

   Article

   Wang et al Nat Cell Biol. 2014 Apr;16(4):345-56. do

   Insert

   KRT5 (KRT5 Human)
   

   Use

   Tags

   mRFP1

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 19, 2014

   Availability

   Academic Institutions and Nonprofits only

8. pCMX-PL1-WDR5

   Plasmid

   #59970

   Purpose

   Mammalian Expression of WDR5

   Depositor

   Debu Chakravarti

   Article

   Kim et al Mol Cell. 2014 May 22;54(4):613-25. doi:

   Insert

   WDR5 (WDR5 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 8, 2014

   Availability

   Academic Institutions and Nonprofits only

9. pLenti6/V5-DEST-FASCIN

   Plasmid

   #31207

   Depositor

   Lynda Chin

   Article

   Scott et al Cancer Cell. 2011 Jul 12;20(1):92-103.

   Insert

   FASCIN (FSCN1 Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 29, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLKO-RFP-IKZF1-sh2

    Plasmid

    #69041

    Purpose

    3rd generation lentiviral vector expressing IKZF1 shRNA

    Depositor

    William Kaelin

    Article

    Lu et al Science. 2014 Jan 17;343(6168):305-9. do

    Insert

    IKZF1 (IKZF1 Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Oct. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. Alpha MHC-DN.JNK2

    Plasmid

    #51931

    Purpose

    mammalian expression of dominant negative JNK2

    Depositor

    Jeffery Molkentin

    Article

    Liang et al EMBO J. 2003 Oct 1;22(19):5079-89.

    Insert

    DN JNK2 (Mapk9 Mouse)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    dominant negative: the dual phosphorylation moti…

    Promoter

    cardiac-specific α-myosin heavy chain 5.5 kb pro…

    Available since

    June 17, 2014

    Availability

    Academic Institutions and Nonprofits only

12. plenti-UBC- IRF4-3xHA-pGK-PUR

    Plasmid

    #107386

    Purpose

    Lentiviral expression of IRF4-3xHA

    Depositor

    William Kaelin

    Article

    Lu et al Science. 2014 Jan 17;343(6168):305-9. do

    Insert

    IRF4 (IRF4 Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    UBC

    Available since

    May 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pKLV2-U6gRNA5(Pou5f1-g2)-PGKpuroBFP-W

    Plasmid

    #105036

    Purpose

    Lentiviral gRNA plasmid targeting mouse Pou5f1 , co-expression of TagBFP

    Depositor

    Kosuke Yusa

    Article

    Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

    Insert

    Pou5f1 (Pou5f1 Mouse)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pVITRO1-dV-IgE/λ

    Plasmid

    #61879

    Purpose

    Human immunoglobulin epsilon antibody expression vector (lambda light chain) without variable regions. Enables Colony-PCR screening of false-positive (PCR vector template) colonies

    Depositor

    Andrew Beavil

    Article

    Dodev et al Sci Rep. 2014 Jul 30;4:5885. doi: 10.103

    Inserts

    Human immunoglobulin Epsilon constant region
    Human immunoglobulin lambda constant region
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Deleted Variable region

    Promoter

    mEF1 Prom and rEF1 Prom

    Available since

    March 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3-HER3-DMVF-181-R

    Plasmid

    #87621

    Purpose

    C-terminal Dronpa fragment fused to the C-terminus of full-length HER3; for BiFC.

    Depositor

    Jin Zhang

    Article

    Hertel et al Cell Rep. 2016 Jan 12;14(2):390-400. doi

    Insert

    HER3-DMVF181-C (ERBB3 Synthetic, Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. 426Gal-FUS

    Plasmid

    #29591

    Depositor

    Aaron Gitler

    Article

    Sun et al PLoS Biol. 2011 Apr;9(4):e1000614. doi:

    Insert

    FUS (FUS Human)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    July 1, 2011

    Availability

    Academic Institutions and Nonprofits only

17. plenti-UbcP-IKZF3-3xHA-pGK-Pur

    Plasmid

    #69053

    Purpose

    lentiviral expression of IKZF3 with HA tag

    Depositor

    William Kaelin

    Article

    Lu et al Science. 2014 Jan 17;343(6168):305-9. do

    Insert

    IKZF3 (IKZF3 Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    UBC

    Available since

    May 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3-Venus-173-N-ORAI1

    Plasmid

    #87618

    Purpose

    N-terminal Venus (YFP) fragment fused to full-length ORAI1; for BiFC.

    Depositor

    Jin Zhang

    Article

    Hertel et al Cell Rep. 2016 Jan 12;14(2):390-400. doi

    Insert

    Venus173-N-ORAI1 (ORAI1 Synthetic, Human)
    

    Use

    Tags

    6xHis, T7 tag (gene 10 leader), and Xpress(TM) ta…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLKO-RFP-IKZF1-sh3

    Plasmid

    #69042

    Purpose

    Lentiviral expression of IKZF1 shRNA

    Depositor

    William Kaelin

    Article

    Lu et al Science. 2014 Jan 17;343(6168):305-9. do

    Insert

    IKZF1 (IKZF1 Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Oct. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. NES-EGFP-cPHx2

    Plasmid

    #116854

    Purpose

    PI(3,4)P2 biosensor

    Depositor

    Gerry Hammond

    Article

    Goulden et al J Cell Biol. 2019 Mar 4;218(3):1066-1079

    Insert

    PLEKHA1 (PLEKHA1 Human)
    

    Use

    Tags

    EGFP and X.leavis map2k1.L(32-44)

    Expression

    Mammalian

    Mutation

    amino acids 169-329 (tandem dimer)

    Promoter

    Available since

    Oct. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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