We narrowed to 30,263 results for: ple

Showing: 3981 - 4000 of 30263 results

Results per page:

Sort results by:

pPpT4- HTA1prom-TIR1-FLAG

Plasmid

#189725

Purpose

Enables expression of OsTIR1 from the HTA1 promoter, used to implement the AID system in Komagataella phaffii (original name pLLpLL001)

Depositor

Anita Emmerstorfer-Augustin

Article

Lehmayer et al iScience. 2022 Aug 6;25(9):104888. doi:

Insert

OsTIR1

Tags

FLAG

Expression

Yeast

Promoter

HTA1

Available Since

Sept. 20, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pPpT4- TEF2prom-TIR1-FLAG

Plasmid

#189726

Purpose

Enables expression of OsTIR1 from the TEF2 promoter, used to implement the AID system in Komagataella phaffii (original name pLL002)

Depositor

Anita Emmerstorfer-Augustin

Article

Lehmayer et al iScience. 2022 Aug 6;25(9):104888. doi:

Insert

OsTIR1

Tags

FLAG

Expression

Yeast

Promoter

TEF2

Available Since

Sept. 20, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pT-GFP-rG1

Plasmid

#188967

Purpose

IPTG inducible GFP with sgRNA

Depositor

Seung-Goo Lee

Article

Kim et al Nucleic Acids Res. 2022 Dec 9;50(22):131

Inserts

GFP
sgRNA: agtggaaaacaatgcgaccgactagt

Use

Synthetic Biology

Expression

Bacterial

Promoter

Ptrc

Available Since

Sept. 12, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pT-GFP-rG2

Plasmid

#188968

Purpose

IPTG inducible GFP with sgRNA

Depositor

Seung-Goo Lee

Article

Kim et al Nucleic Acids Res. 2022 Dec 9;50(22):131

Inserts

GFP
sgRNA: agtccatgtaatcagcgtctactagt

Use

Synthetic Biology

Expression

Bacterial

Promoter

Ptrc

Available Since

Sept. 9, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
L1_NOP1:mS-N7-Ck2

Plasmid

#136126

Purpose

L1 in position 2, example of weak promoter

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

p5-MpNOP1:mScarletI-N7_3tNos-35S

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI/ SapI domesticated

Available Since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
FKBP-EGFP-PML peptide

Plasmid

#175247

Purpose

Bacterial expression and purification, low affinity SUMOylation substrate with a relatively high Km, can be recruited to FRB with rapamycin

Depositor

Michael Rosen

Article

Peeples et al Nat Chem Biol. 2021 Jun;17(6):693-702. d

Insert

PML (PML Human)

Expression

Bacterial

Promoter

tac

Available Since

Oct. 22, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pVD2

Plasmid

#165857

Purpose

universal Vector Domesticator vector v2 used in the integration (domestication) of new vector backbone parts in the GB2.0 cloning system. Uses blue-white screening method

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Type

Empty backbone

Use

Synthetic Biology

Available Since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pSWD95

Plasmid

#158617

Purpose

CckA FRET Sensor

Depositor

Seth Childers

Article

Duvall et al ACS Sens. 2020 Jun 26;5(6):1589-1596. do

Insert

CckA 1-182, Linker SNVTRHRSATE, mClover3, CckA 183-691, Linker SNVTRHRSAT, mRuby3

Expression

Bacterial

Promoter

Xylose

Available Since

Nov. 17, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pZS160

Plasmid

#134238

Purpose

Lentiviral plasmid expressing sgRNA against HJURP

Depositor

Iain Cheeseman

Article

Swartz et al Dev Cell. 2019 Oct 7;51(1):35-48.e7. doi

Insert

Human HJURP sgRNA

Use

Lentiviral

Available Since

Feb. 12, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pRPL28 CaURA3 (pNTI592)

Plasmid

#115431

Purpose

URA3-marked RPS28

Depositor

Nicholas Ingolia

Article

McGeachy et al ACS Synth Biol. 2019 Apr 19;8(4):844-856

Inserts

RPL28 (RPL28 Budding Yeast)
URA3

Expression

Yeast

Mutation

KpnI site in intron

Promoter

Ashbya gossypii and endogenous

Available Since

Nov. 26, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
MSCV-6NBC-GFP-PGK-Puro-Barcode9

Plasmid

#83686

Purpose

individual 6N barcode to make barcoded cell line variants

Depositor

Monte Winslow

Article

Gruner et al Nat Methods. 2016 Sep 12. doi: 10.1038/n

Insert

Barcode9

Use

Retroviral

Mutation

please view depositor comments below

Available Since

Nov. 10, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pVH004

Plasmid

#80401

Purpose

Contains pBAD-CusR and pCusR-antiscaffold with no fluorescent protein. Used as a control for autofluorescence.

Depositor

Richard Murray

Article

Hsiao et al ACS Synth Biol. 2015 Feb 20;4(2):150-61.

Inserts

CusR (cusR )
AraC (araC )
Anti-scaffold

Use

Synthetic Biology

Tags

C-terminal LZx domain

Expression

Bacterial

Available Since

Oct. 13, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pVH015

Plasmid

#80398

Purpose

Contains pBAD-CusR (response regulator) and pCusR-antiscaffold-GFP(3xFLAG). Used for Western blots

Depositor

Richard Murray

Article

Hsiao et al ACS Synth Biol. 2015 Feb 20;4(2):150-61.

Inserts

Anti-scaffold-ffGFP-3xFLAG
CusR (cusR )
AraC (araC )

Use

Synthetic Biology

Tags

C-terminal 3xFLAG tag and C-terminal LZx domain

Expression

Bacterial

Available Since

Sept. 19, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-myc-mCE

Plasmid

#82459

Purpose

Expression of myc epitope and murine mRNA Capping Enzyme in mammalian cells

Depositor

Daniel Schoenberg

Article

Otsuka et al Mol Cell Biol. 2009 Apr;29(8):2155-67. d

Insert

mRNA capping enzyme (Rngtt Mouse)

Tags

myc

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 8, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pJexpress414_OmpA171_WTnoGirdles

Plasmid

#62653

Purpose

Wild-type OmpA with aromatic girdle residues replaced by leucine

Depositor

Timothy Whitehead

Article

Stapleton et al Proc Natl Acad Sci U S A. 2015 Jul 21. p

Insert

OmpA_noGirdles

Use

Synthetic Biology

Tags

6His and FlAsH

Expression

Bacterial

Mutation

Aromatic girdle residues mutated to Leu (W7, W15,…

Promoter

T7

Available Since

May 27, 2015

Availability

Academic Institutions and Nonprofits only
pX330-U6-Chimeric_BB-CBh-hSpCas9

Plasmid

#42230

Purpose

A human codon-optimized SpCas9 and chimeric guide RNA expression plasmid.

Depositor

Feng Zhang

Article

Cong et al Science. 2013 Jan 3.

Has Service

Cloning Grade DNA

Insert

humanized S. pyogenes Cas9

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Promoter

CBh

Available Since

Feb. 27, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pMK277 (mClover-NeoR)

Plasmid

#72793

Purpose

C-terminal tagging with mClover using CRIPSR/Cas9

Depositor

Masato Kanemaki

Article

Natsume et al Cell Rep. 2016 Apr 5;15(1):210-8. doi: 1

Insert

mClover-Neo

Use

CRISPR

Tags

mClover

Expression

Mammalian

Available Since

March 24, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pSLQ8703 pHR-R(TRE3G-Fluc-2XcrIFNg-SV40pA)-EF1a-sfGFP-T2A-rtta-WPRE

Plasmid

#140230

Purpose

Dox inducible expression of luciferase + crIFNg. Constitutive EF1a driven rtTA and GFP

Depositor

Stanley Qi

Article

Kempton et al Mol Cell. 2020 Jan 30. pii: S1097-2765(2

Insert

Luciferase + crIFNg (reverse orientation)

Use

Lentiviral

Expression

Mammalian

Promoter

TRE3G

Available Since

April 21, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pMRXIP-GFP-Evectin-2

Plasmid

#221754

Purpose

Stable expression of Evectin-2 in mammalian cells

Depositor

Noboru Mizushima

Article

Shinoda et al Elife. 2024 Jun 4;12:RP92189. doi: 10.75

Insert

PLEKHB2 (PLEKHB2 Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Mutation

tandem PLEKHB2(PH domain) (amino acids 1-110)

Available Since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pIRE4-Azi

Plasmid

#105829

Purpose

Plasmid encoding for the translational pair that enables efficent incoporation of p-Azido-phenylalanine in response to the amber codon into proteins in mammmalian cells

Depositor

Irene Coin

Article

Seidel et al Elife. 2017 Aug 3;6. doi: 10.7554/eLife.

Inserts

Humanized EAziRS
4 copies of the U6-BstYam tRNA cassette

Expression

Mammalian

Mutation

(Y37L, D182S, F183M, L186A, D265R)TyrRS

Promoter

PGK and U6

Available Since

March 15, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge