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ACVR2B gRNA (BRDN0001147208)

Plasmid

#75485

Purpose

3rd generation lentiviral gRNA plasmid targeting human ACVR2B

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

ACVR2B (ACVR2B Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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ACVR2B gRNA (BRDN0001149036)

Plasmid

#75486

Purpose

3rd generation lentiviral gRNA plasmid targeting human ACVR2B

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

ACVR2B (ACVR2B Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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Jam3-Fc-His

Plasmid

#72080

Purpose

Expresses the extracellular region of the JAM-3 protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Jam3 (Jam3 Mouse)

Tags

Fc-His

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 12, 2016

Availability

Academic Institutions and Nonprofits only

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Jam3-AP-His

Plasmid

#71954

Purpose

Expresses the extracellular region of the JAM-3 protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Jam3 (Jam3 Mouse)

Tags

AP-His

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 2, 2016

Availability

Academic Institutions and Nonprofits only

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Nrp2.2-AP-His

Plasmid

#71973

Purpose

Expresses the extracellular region of the Neuropilin 2, isoform 2 protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Nrp2.2 (Nrp2 Mouse)

Tags

AP-His

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 1, 2016

Availability

Academic Institutions and Nonprofits only

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Nrp2.6-AP-His

Plasmid

#71977

Purpose

Expresses the extracellular region of the Neuropilin 2, isoform 6 protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Nrp2.6 (Nrp2 Mouse)

Tags

AP-His

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 25, 2016

Availability

Academic Institutions and Nonprofits only

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Nrp2.5-AP-His

Plasmid

#71976

Purpose

Expresses the extracellular region of the Neuropilin 2, isoform 5 protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Nrp2.5 (Nrp2 Mouse)

Tags

AP-His

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 22, 2016

Availability

Academic Institutions and Nonprofits only

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Nrp2.4-AP-His

Plasmid

#71975

Purpose

Expresses the extracellular region of the Neuropilin 2, isoform 4 protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Nrp2.4 (Nrp2 Mouse)

Tags

AP-His

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 22, 2016

Availability

Academic Institutions and Nonprofits only

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pCMV-myc-mIFITM3-Y20,27A

Plasmid

#58465

Purpose

Expresses murine IFITM3-Y20,27A with an N-terminal myc tag in mammalian cells.

Depositor

Jacob Yount

Article

Chesarino et al J Biol Chem. 2014 Apr 25;289(17):11986-9

Insert

IFITM3 (Ifitm3 Mouse)

Tags

myc

Expression

Mammalian

Mutation

Tyrosines 20 and 27 to alanine

Promoter

CMV IE

Available Since

Sept. 9, 2014

Availability

Academic Institutions and Nonprofits only
pCMV-myc-mIFITM3-Y27A

Plasmid

#58464

Purpose

Expresses murine IFITM3-Y27A with an N-terminal myc tag in mammalian cells.

Depositor

Jacob Yount

Article

Chesarino et al J Biol Chem. 2014 Apr 25;289(17):11986-9

Insert

IFITM3 (Ifitm3 Mouse)

Tags

myc

Expression

Mammalian

Mutation

Tyrosine 27 to alanine

Promoter

CMV IE

Available Since

Sept. 9, 2014

Availability

Academic Institutions and Nonprofits only
p198 Dync1i2.F (Ex1a)

Plasmid

#26448

Depositor

Elizabeth Fisher

Article

Kuta et al PLoS One. 2010 . 5(7):e11682.

Insert

cytoplasmic dynein 1 intermediate chain 2 isoform F (exon 1a) (Dync1i2 Mouse)

Use

Subcloning and sequencing

Mutation

N/A

Available Since

March 24, 2011

Availability

Academic Institutions and Nonprofits only
Ef1a-VP16-PYL-T2A-GAL4_DBD-ABI-pPGK-mCherry (pSS057)

Plasmid

#236348

Purpose

Second generation lentivirus compatible transfer vector encoding an NS3 gene expression system and an mCherry transduction marker

Depositor

Hana El-Samad

Insert

Ef1a-VP16-PYL-T2A-GAL4_DBD-ABI-pPGK-mCherry

Use

Lentiviral

Promoter

Ef1a; PGK

Available Since

June 12, 2026

Availability

Academic Institutions and Nonprofits only

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pCvL31-NanoLuc^opt

Plasmid

#255782

Purpose

Expresses NanoLuc luciferase (codon optimized for Chromera) in Chromera velia

Depositor

Ke Hu

Article

Tengganu et al J Cell Sci. 2026 Jan 1;139(1):jcs.264400

Insert

Cv β-tubulin intron 1 + GAPDH Kozak + NanoLuc

Use

Chromera velia, toxoplasma gondii

Expression

Bacterial

Mutation

codon optimized for Chromera velia

Promoter

putative C. velia L31 promoter (1103 bp)

Available Since

June 10, 2026

Availability

Academic Institutions and Nonprofits only

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pscAAV-KIKO-U6-gTgfbr2-p2a-mCherry-STOPpA-400

Plasmid

#249131

Purpose

all-in-one HDRT-based knockin-knockout (KIKO) design to insert mCherry (=knockin) at gTgfbr2 cut site, disrupting Osmr expression (=knockout)

Depositor

Matthew Krummel

Article

Kuhn et al J Exp Med. 2026 May 4;223(5):e20251228.

Insert

gTgfbr2, mCherry (Tgfbr2 Mouse, Synthetic)

Use

AAV

Available Since

March 4, 2026

Availability

Academic Institutions and Nonprofits only

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pMSCV-IRES-DsRed/MPL

Plasmid

#214793

Purpose

Mammalian expression of human MPL

Depositor

Norio Komatsu

Article

Masubuchi et al Haematologica. 2025 Nov 6. doi: 10.3324/

Insert

human MPL (MPL Human)

Use

Retroviral

Tags

non

Promoter

MSCV

Available Since

Dec. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pCW-CKS2-HA

Plasmid

#247439

Purpose

HA-tagged mouse CKS2 cloned into the pCW backbone (Addgene #41393, with puromycin resistance) together with an IRES GFP to indicate doxycycline-inducible expression by fluorescence.

Depositor

Richard Treisman

Article

Nielsen et al J Cell Sci. 2025 Nov 7:jcs.264444. doi:

Inserts

CKS2 (Cks2 Mouse)
IRES2:EGFP

Use

Lentiviral; Dox-inducible

Tags

HA

Expression

Mammalian

Available Since

Dec. 4, 2025

Availability

Academic Institutions and Nonprofits only

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Human Coxsackievirus A16 strain G10 inactive 2A protease

Plasmid

#228665

Purpose

Bacterial expression of the A71 2A protease, innactive and can be used as a control in assays

Depositor

ASAP Discovery, Frank von Delft

Insert

Enterovirus A71 2A protease

Tags

His-SUMO

Expression

Bacterial

Mutation

Avitag insertion and C2A mutations / Active site …

Available Since

Feb. 18, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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5'-3xFLAG Zmynd8-exon minimal CMV pCDNA5

Plasmid

#212078

Purpose

LR vector for integration of Zmynd8-exon into N2a FRT rtTA3 expression cells

Depositor

Benjamin Blencowe

Article

Roth et al Mol Cell. 2023 Dec 7;83(23):4222-4238.e1

Insert

Zmynd8-exon (Zmynd8 Mouse)

Expression

Mammalian

Available Since

Feb. 7, 2024

Availability

Academic Institutions and Nonprofits only

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ABE-RA5.0

Plasmid

#208312

Purpose

Expresses ABE-RA5.0 in mammalian cells

Depositor

Weixin Tang

Article

Xiao et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

Insert

TadA-RA5.0-SpCas9 D10A

Expression

Mammalian

Mutation

W23R, H36L, R47K, P48A, R51L, I76F, V82S, A106V, …

Available Since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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ABE-RA5.1

Plasmid

#208313

Purpose

Expresses ABE-RA5.1 in mammalian cells

Depositor

Weixin Tang

Article

Xiao et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

Insert

TadA-RA5.1-SpCas9 D10A

Expression

Mammalian

Mutation

W23R, H36L, R47K, P48A, R51L, I76F, V82S, A106V, …

Available Since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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