We narrowed to 5,405 results for: dre

1. pGEX6P1-DEST-HA

   Plasmid

   #119751

   Purpose

   Allows Gateway cloning of gene of interest into bacterial expression vector with PreScission Protease cleavable N-terminal GST tag, and a retained C-terminal HA tag.

   Depositor

   Martin Reijns, Andrew Jackson

   Type

   Empty backbone

   Use

   Tags

   GST and HA

   Expression

   Bacterial

   Mutation

   Promoter

   tac promoter

   Available since

   Jan. 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCZ829

   Plasmid

   #110137

   Purpose

   Epidermal Mitochondrial-tagged Florescent Calcium Sensor

   Depositor

   Andrew Chisholm

   Article

   Xu et al Dev Cell. 2014 Oct 13;31(1):48-60. doi:

   Insert

   mito-GCaMP5
   

   Use

   Tags

   mito::GCaMP5

   Expression

   Worm

   Mutation

   Promoter

   Pcol-19

   Available since

   May 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pQE-WT-T3-R5-RRVH

   Plasmid

   #63623

   Purpose

   T3-R5-RRVH RNAP mutant

   Depositor

   Andrew Ellington

   Article

   Meyer et al ACS Synth Biol. 2014 Oct 7.

   Insert

   T3 RRVH RNAP
   

   Use

   Tags

   Expression

   Mutation

   T745K, N748D, L749M, M750I, G753R, H772R, E775V, …

   Promoter

   T5/Lac

   Available since

   July 29, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. GFP-I48 ELP

   Plasmid

   #67857

   Purpose

   GFP Fluorescent Elastin-like Polypeptide(VPGIG) with 48 repeats containing Isoleucine guest residues

   Depositor

   Andrew MacKay

   Insert

   GFP fused to ELP (VPGIG) repeated 48 times
   

   Use

   Tags

   GFP fused to N-terminal of ELP

   Expression

   Mammalian

   Mutation

   Promoter

   T7

   Available since

   Oct. 1, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJA318

   Plasmid

   #74489

   Purpose

   eF1alpha::puroR::mCherry::T2A::eGFP::d4ODC::wPRE has d4ODC degron

   Depositor

   Andrew Fire

   Article

   Arribere et al Nature. 2016 Jun 1;534(7609):719-723. do

   Insert

   d4ODC
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 27, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRB1081

   Plasmid

   #71479

   Purpose

   Expresses gRNA for dpy-10 conversion using NGAG PAM and VQR Cas9 variant

   Depositor

   Andrew Fire

   Article

   Bell et al Genetics. 2015 Dec 17. pii: genetics.115

   Insert

   dpy-10 gRNA
   

   Use

   CRISPR

   Tags

   Expression

   Worm

   Mutation

   Promoter

   U6

   Available since

   Dec. 10, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pQE-WT-KIF-R5-IRH

   Plasmid

   #63629

   Purpose

   K1F-R5-IRH RNAP mutant

   Depositor

   Andrew Ellington

   Article

   Meyer et al ACS Synth Biol. 2014 Oct 7.

   Insert

   KIF-R5-IRH RNAP
   

   Use

   Tags

   Expression

   Mutation

   L749I, Q754S, R756N, I761V, H772R, and Q786H

   Promoter

   T5/Lac

   Available since

   June 1, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. PX398 Nitratifractor salsuginis str DSM 16511 Cas9

   Plasmid

   #68334

   Purpose

   Nitratifractor salsuginis str DSM 16511 Cas9

   Depositor

   Feng Zhang

   Insert

   NsCas9
   

   Use

   CRISPR

   Tags

   HA and NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 26, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pQFD

   Plasmid

   #48101

   Purpose

   Destination plasmid (Gateway cloning) for cumate-inducible expression of 3xFLAG-tagged fusion protein

   Depositor

   Julia Vorholt

   Article

   Kaczmarczyk et al Appl Environ Microbiol. 2013 Nov;79(21):

   Type

   Empty backbone

   Use

   Tags

   3xFLAG

   Expression

   Bacterial

   Mutation

   Promoter

   PQ5

   Available since

   Jan. 28, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pQHD

    Plasmid

    #48102

    Purpose

    Destination plasmid (Gateway cloning) for cumate-inducible expression of HA-tagged fusion protein

    Depositor

    Julia Vorholt

    Article

    Kaczmarczyk et al Appl Environ Microbiol. 2013 Nov;79(21):

    Type

    Empty backbone

    Use

    Tags

    HA tag

    Expression

    Bacterial

    Mutation

    Promoter

    PQ5

    Available since

    Jan. 28, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET29b- pchB WT

    Plasmid

    #70099

    Purpose

    expresses isochorismate-pyruvate lyase from Pseudomonas aeruginosa

    Depositor

    Audrey Lamb

    Article

    Zaitseva et al J Biol Chem. 2006 Nov 3;281(44):33441-9.

    Insert

    pchB
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 13, 2015

    Availability

    Academic Institutions and Nonprofits only

12. pQYD

    Plasmid

    #48104

    Purpose

    Destination plasmid (Gateway cloning) for cumate-inducible expression of SYFP2-tagged fusion protein

    Depositor

    Julia Vorholt

    Article

    Kaczmarczyk et al Appl Environ Microbiol. 2013 Nov;79(21):

    Type

    Empty backbone

    Use

    Tags

    SYFP2

    Expression

    Bacterial

    Mutation

    Promoter

    PQ5

    Available since

    Jan. 28, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCZ47

    Plasmid

    #128414

    Purpose

    vab-1 rescuing minigene

    Depositor

    Andrew Chisholm

    Article

    George et al Cell. 1998 Mar 6;92(5):633-43.

    Insert

    vab-1 (vab-1 Nematode)
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pRB1084

    Plasmid

    #71516

    Purpose

    Expresses gRNA for dpy-10 conversion using NGCG PAM and VRER Cas9 mutant

    Depositor

    Andrew Fire

    Article

    Bell et al Genetics. 2015 Dec 17. pii: genetics.115

    Insert

    dpy-10 gRNA
    

    Use

    CRISPR

    Tags

    Expression

    Worm

    Mutation

    Promoter

    U6

    Available since

    Dec. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pFUSC9

    Plasmid

    #50429

    Purpose

    Plasmid compatible with the Golden Gate TALEN cloning system. To clone TAL effectors 9.5 RVDs long.

    Depositor

    Andrew Scharenberg

    Article

    Boissel et al Nucleic Acids Res. 2013 Nov 26.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET28_Strep-Psc-pScaC

    Plasmid

    #228834

    Purpose

    E. coli expression of the ScaC passenger domain

    Depositor

    Andrew Carter

    Article

    Manigrasso et al bioRxiv 2024.04.11.589026

    Insert

    ScaC
    

    Use

    Tags

    Strep

    Expression

    Bacterial

    Mutation

    aa 33-223

    Promoter

    Available since

    Jan. 31, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET28_pScaC(50-159)

    Plasmid

    #228836

    Purpose

    E. coli expression of a truncated ScaC passenger domain (aa 50-159)

    Depositor

    Andrew Carter

    Article

    Manigrasso et al bioRxiv 2024.04.11.589026

    Insert

    ScaC
    

    Use

    Tags

    Strep

    Expression

    Bacterial

    Mutation

    aa 50-159

    Promoter

    Available since

    Jan. 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDL1530

    Plasmid

    #226636

    Purpose

    This plasmid is for the induced expression of nrsB (formerly known as ORF229) from ICP1_2001.

    Depositor

    Andrew Camilli

    Article

    Woldetsadik et al mBio. 2024 Oct 8:e0245724. doi: 10.1128/

    Insert

    nrsB (ORF 229)
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pgRNA_AT

    Plasmid

    #196243

    Purpose

    Backbone plasmid for cloning sgRNA

    Depositor

    Andrea Puhar

    Article

    Sharma et al mSystems. 2022 Dec 21:e0104522. doi: 10.

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23119

    Available since

    Nov. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAM299

    Plasmid

    #224258

    Purpose

    MCMUbHis

    Depositor

    Andreas Martin

    Article

    Williams et al Mol Cell. 2023 Mar 2;83(5):759-769.e7. d

    Insert

    MC-Ub-3C-His
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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