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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 8,640 results for: dykddddk

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Showing: 4001 - 4020 of 8640 results

  1. JG676: CAG-human dLbCpf1(D832A)-NLS-3xHA-3xFLAG-DmrA(X2)

    Plasmid
    #104569
    Purpose
    Mammalian expression vector for catalytically inactive Cpf1 from Lachnospiraceae bacterium (dLbCpf1) fused to two DmrA domains
    Depositor
    Keith Joung
    Publication
    Tak et al Nat Methods. 2017 Oct 30. doi: 10.1038/n
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dLbCpf1(D832A)-DmrA(x2)
    Tags
    3x FLAG, 3x HA, and NLS
    Expression
    Mammalian
    Mutation
    D832A
    Promoter
    CAG
    Available since
    March 29, 2018
    Availability
    Academic Institutions and Nonprofits only
    104569-plasmid-map-sequence-id-201433

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  2. JG674: CAG-human dLbCpf1(D832A)-NLS-3xHA-3xFLAG-DmrA(X1)

    Plasmid
    #104568
    Purpose
    Mammalian expression vector for catalytically inactive Cpf1 from Lachnospiraceae bacterium (dLbCpf1) fused to one DmrA domain
    Depositor
    Keith Joung
    Publication
    Tak et al Nat Methods. 2017 Oct 30. doi: 10.1038/n
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dLbCpf1(D832A)-DmrA(x1)
    Tags
    3x FLAG, 3x HA, and NLS
    Expression
    Mammalian
    Mutation
    D832A
    Promoter
    CAG
    Available since
    March 29, 2018
    Availability
    Academic Institutions and Nonprofits only
    104568-plasmid-map-sequence-id-201431

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  7. ciCas9(F22)_pcDNA5

    Plasmid
    #100552
    Purpose
    Expresses ciCas9(F22) in mammalian cells. Can be used to generate Flp-In and Flp-In T-REx stables.
    Depositor
    Dustin Maly
    Publication
    Rose et al Nat Methods. 2017 Jul 24. doi: 10.1038/n
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ciCas9(F22)
    Use
    CRISPR; Frt, flp-in
    Tags
    FLAG
    Expression
    Mammalian
    Available since
    Sept. 22, 2017
    Availability
    Academic Institutions and Nonprofits only
    100552-plasmid-map-sequence-id-193861

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  13. LP455

    Plasmid
    #90198
    Purpose
    pFLAG-CMV2 with cDNA encoding HsNPHP4 aa residues 649-1426
    Depositor
    Lotte Pedersen
    Publication
    Schou et al Nat Commun. 2017 Jan 30;8:14177. doi: 10
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    NPHP4 (NPHP4 Human)
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    aa residues 649-1426
    Available since
    May 16, 2017
    Availability
    Academic Institutions and Nonprofits only
    90198-plasmid-map-sequence-id-178306

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  15. C-BLa 1.1

    Plasmid
    #88997
    Purpose
    BLaTM, a genetic tool to measure homo- and heterotypic transmembrane helix-helix interactions. Plasmid encoding the 1.1 fusionprotein containing the C-terminal fragment of the split β-lactamase.
    Depositor
    Dieter Langosch
    Publication
    Schanzenbach et al Sci Rep. 2017 Mar 7;7:43476. doi: 10.103
    Insert
    C-BLa 1.1 fusion protein
    Tags
    Flag
    Expression
    Bacterial
    Promoter
    araBAD
    Available since
    May 2, 2017
    Availability
    Academic Institutions and Nonprofits only
    88997-plasmid-map-sequence-id-177504

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  18. pAcrF1

    Plasmid
    #89233
    Purpose
    Plasmid contains the gene for anti-CRISPR protein AcrF1 (gene 35 from bacteriophage JBD30), with N-terminal 6his tag and TEV protease cleavage site
    Depositor
    Blake Wiedenheft
    Publication
    Chowdhury et al Cell. 2017 Mar 23;169(1):47-57.e11. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    AcrF1
    Tags
    6xHis-TEV and FLAG
    Available since
    April 28, 2017
    Availability
    Academic Institutions and Nonprofits only
    89233-plasmid-map-sequence-id-177171

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  19. FLAG-hAR-508-660

    Plasmid
    #89138
    Purpose
    mammalian expression of FLAG-tagged human androgen receptor amino acid residues 508-660 in pCMV5-FLAG
    Depositor
    Elizabeth Wilson
    Insert
    hAR-508-660 (AR Human)
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    contains aa 508-660
    Promoter
    CMV
    Available since
    April 11, 2017
    Availability
    Academic Institutions and Nonprofits only
    89138-plasmid-map-sequence-id-176164

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Showing: 4001 - 4020 of 8640 results