We narrowed to 13,532 results for: 11

1. pFM113

   Plasmid

   #40159

   Depositor

   Geraldine Seydoux

   Article

   Motegi et al Nat Cell Biol. 2011 Oct 9;13(11):1361-7.

   Insert

   par-3N (par-3, par-3 Nematode)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   N terminal portion of protein (aa1-364)

   Promoter

   tac

   Available since

   Oct. 23, 2012

   Availability

   Academic Institutions and Nonprofits only

2. pFM114

   Plasmid

   #40160

   Depositor

   Geraldine Seydoux

   Article

   Motegi et al Nat Cell Biol. 2011 Oct 9;13(11):1361-7.

   Insert

   par-3N+S251A (par-3 Nematode)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   N terminal portion of protein (aa 1-364); S251A

   Promoter

   tac

   Available since

   Oct. 23, 2012

   Availability

   Academic Institutions and Nonprofits only

3. pFM116

   Plasmid

   #40162

   Depositor

   Geraldine Seydoux

   Article

   Motegi et al Nat Cell Biol. 2011 Oct 9;13(11):1361-7.

   Insert

   par-3C+S950A (par-3 Nematode)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   C terminal portion of protein (aa840-1268); S950A

   Promoter

   tac

   Available since

   Sept. 12, 2012

   Availability

   Academic Institutions and Nonprofits only

4. pSL7 SMR1791

   Plasmid

   #28030

   Depositor

   Susan Rosenberg

   Article

   Harris et al Genes Dev. 1997 Sep 15. 11(18):2426-37.

   Insert

   MutS, MutL (mutL, mutS E. coli)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 1, 2011

   Availability

   Academic Institutions and Nonprofits only

5. pWZ414-F48

   Plasmid

   #23072

   Depositor

   Sharon Dent

   Article

   Zhang et al EMBO J. 1998 Jun 1. 17(11):3155-67.

   Insert

   yeast Histone H3-2 and Histone H4-2
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Histone H3 changed Lysine 14 to Glutamine, H3 K14…

   Promoter

   Available since

   March 3, 2010

   Availability

   Academic Institutions and Nonprofits only

6. pWZ414-F50

   Plasmid

   #23074

   Depositor

   Sharon Dent

   Article

   Zhang et al EMBO J. 1998 Jun 1. 17(11):3155-67.

   Insert

   yeast Histone H3-2 and Histone H4-2
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Histone H3 changed Lysine 14 to Arginine, H3 K14R…

   Promoter

   Available since

   Feb. 17, 2010

   Availability

   Academic Institutions and Nonprofits only

7. pWZ414-F54

   Plasmid

   #23078

   Depositor

   Sharon Dent

   Article

   Zhang et al EMBO J. 1998 Jun 1. 17(11):3155-67.

   Insert

   yeast Histone H3-2 and Histone H4-2
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Histone H3 changed Lysine 9 to Glutamine, H3 K9Q …

   Promoter

   Available since

   Feb. 17, 2010

   Availability

   Academic Institutions and Nonprofits only

8. pES1004

   Plasmid

   #21790

   Depositor

   Richard Yu

   Article

   Yu et al Nature. 2008 Dec 11. 456(7223):755-61.

   Insert

   Kss1-as2-trunc (KSS1 Budding Yeast)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   deleted bases 1-14 from open reading frame E94A …

   Promoter

   Available since

   Oct. 1, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pILGFP3AE4

   Plasmid

   #185873

   Purpose

   A plasmid for in vivo gene amplification (HamAmp) at RPL25 locus (~11 copy)

   Depositor

   Claudia Vickers

   Type

   Empty backbone

   Use

   Tags

   Expression

   Yeast

   Mutation

   None

   Promoter

   Available since

   Sept. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pRSV-Rev

    Plasmid

    #12253

    Purpose

    3rd generation lentiviral packaging plasmid; Contains Rev; also requires pMDLg/pRRE (Addgene#12251) and envelope expressing plasmid (Addgene#12259)

    Depositor

    Didier Trono

    Article

    Dull et al J Virol. 1998 Nov . 72(11):8463-71.

    Insert

    Rev (rev )
    

    Use

    Lentiviral; Packaging

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 4, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. lentiCas9-Blast

    Plasmid

    #52962

    Purpose

    Expresses human codon-optimized S. pyogenes Cas9 protein and blasticidin resistance from EFS promoter. 3rd generation lentiviral backbone.

    Depositor

    Feng Zhang

    Article

    Sanjana et al Nat Methods. 2014 Aug;11(8):783-4. doi:

    Has Service

    Lentiviral Prep

    Inserts

    Cas9
    Blasticidin resistance
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    EFS-NS

    Available since

    May 8, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pX330S-2-PITCh

    Plasmid

    #63670

    Purpose

    Expresses Cas9 nuclease and the PITCh-gRNA

    Depositor

    Takashi Yamamoto

    Article

    Sakuma et al Nat Protoc. 2016 Jan;11(1):118-33. doi:

    Insert

    humanized S. pyogenes Cas9
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CBh

    Available since

    Dec. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCRIS-PITChv2-FBL

    Plasmid

    #63672

    Purpose

    PITCh donor vector for EGFP-2A-PuroR knock-in into human FBL locus

    Depositor

    Takashi Yamamoto

    Article

    Sakuma et al Nat Protoc. 2016 Jan;11(1):118-33. doi:

    Insert

    EGFP-2A-PuroR
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Promoterless

    Available since

    Dec. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pEDA5_GFPmut3_Y66H

    Plasmid

    #80085

    Purpose

    Positive control plasmid for Nicking Mutagenesis. Constitutively expresses a non-fluorescent variant of GFPmut3 (Y66H) that is mutated to fluorescent (H66Y) in the protocol.

    Depositor

    Timothy Whitehead

    Article

    Wrenbeck et al Nat Methods. 2016 Nov;13(11):928-930. do

    Insert

    GFPmut3_Y66H
    

    Use

    Tags

    6x-His tag

    Expression

    Bacterial

    Mutation

    changed Tyrosine 66 to Histidine

    Promoter

    proB

    Available since

    July 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pC0090

    Plasmid

    #181954

    Purpose

    Beta catenin reporter M50 Super 8x (TCF/LEF binding sites) TOPFlash with Gluc/Cluc

    Depositor

    Feng Zhang

    Article

    Abudayyeh et al Science. 2019 Jul 11. pii: science.aax70

    Insert

    7xTOPFlash
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pC0091

    Plasmid

    #181955

    Purpose

    Beta catenin reporter control M51 Super 8x (mutated TCF/LEF binding sites) FOPFlash with Gluc/Cluc

    Depositor

    Feng Zhang

    Article

    Abudayyeh et al Science. 2019 Jul 11. pii: science.aax70

    Insert

    7xFOP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pEGFP-Merlin

    Plasmid

    #84293

    Purpose

    Expression of wt Merlin

    Depositor

    Jonathan Chernoff

    Article

    Xiao et al J Biol Chem. 2002 Jan 11. 277(2):883-6.

    Insert

    Merlin (NF2) (NF2 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pMRX-IZ-HaloTag7-CHMP4A

    Plasmid

    #184895

    Purpose

    Stable expression of HaloTag7-CHMP4A in mammalian cells

    Depositor

    Noboru Mizushima

    Article

    Yim et al FEBS Lett. 2022 Mar 11. doi: 10.1002/187

    Insert

    CHMP4A (CHMP4A Human)
    

    Use

    Tags

    HaloTag7

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. Lact-C2-GFP

    Plasmid

    #22852

    Depositor

    Sergio Grinstein

    Article

    Yeung et al Science. 2008 Jan 11. 319(5860):210-3.

    Insert

    Lactadherin-C2 Domain (MFGE8 Bovine)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 15, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pDONRPEX18Tp-SceI-pheS

    Plasmid

    #111608

    Purpose

    Gateway compatible allelic exchange vector for Burkholderia sp.

    Depositor

    Tim Tolker-Nielsen

    Article

    Fazli et al Appl Environ Microbiol. 2015 Jun;81(11):

    Type

    Empty backbone

    Use

    Allelic exchange in burkholderia sp.

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge