We narrowed to 4,215 results for: Bre;

1. pTol2CG2-ubi:QFGal4-SV40pA

   Plasmid

   #155119

   Purpose

   Tol2 vector for ubiquitous expression of QFGal4. Contains cmlc2:mRFP-SV40pA transgenesis marker

   Depositor

   Bret Pearson

   Article

   Burgess et al Dev Biol. 2020 Jul 19. pii: S0012-1606(2

   Insert

   ubi:QFGal4 (ubb Zebrafish, Budding Yeast, Neurospora crassa)
   

   Use

   Zebrafish expression

   Tags

   Expression

   Mutation

   Promoter

   ubiquitin B

   Available since

   Sept. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA5/FRT/TO-GAlphaGustducin-RLuc8

   Plasmid

   #140979

   Purpose

   Encodes a G alpha subunit (GNAT3) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

   Depositor

   Bryan Roth

   Article

   Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

   Insert

   GAlphaGustducin-RLuc8 (GNAT3 Human)
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   RLuc8 and flanking SGGGGS linkers have been inser…

   Promoter

   CMV

   Available since

   June 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pACEBac1-PSMB1-PSMB2-PSMB3-PSMB4-TEV-2xSTII

   Plasmid

   #217897

   Purpose

   Insect cell expression of human 20S CP proteasome subunit beta type-1 (PSMB1), beta type-2 (PSMB2), beta type-3 (PSMB3), and beta type-4 (PSMB4) with C-terminal Twin-Strep-tag

   Depositor

   Brenda Schulman

   Article

   Adolf et al Nat Struct Mol Biol. 2024 Apr 10. doi: 1

   Inserts

   PSMB1 (PSMB1 Human)
   PSMB2 (PSMB2 Human)
   PSMB3 (PSMB3 Human)
   PSMB4 (PSMB4 Human)
   

   Use

   Tags

   2xSTII

   Expression

   Insect

   Mutation

   Promoter

   Available since

   May 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. EGFP-Bcr R1090A

   Plasmid

   #36416

   Depositor

   Nora Heisterkamp, Eunjoon Kim

   Article

   Oh et al J Neurosci. 2010 Oct 20;30(42):14134-44.

   Insert

   breakpoint cluster region protein isoform 1 (BCR Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   R1090A

   Promoter

   Available since

   Aug. 30, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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5. FRB_T2098L-VAMP3-mCherry

   Plasmid

   #92429

   Purpose

   Expression of VAMP3 N-terminally conjugated to FRB and C-terminally conjugated to mCitrine.

   Depositor

   Geert van den Bogaart

   Article

   Verboogen et al Elife. 2017 May 19;6. pii: e23525. doi:

   Insert

   VAMP3 (Vamp3 Mouse)
   

   Use

   Tags

   FKBP rapamycin binding (FRB) domain of mTOR with …

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEW108

   Plasmid

   #232823

   Purpose

   Expresses yeast compass complex in insect cells

   Depositor

   Cynthia Wolberger

   Article

   Worden et al Elife. 2020 Jan 10;9. pii: 53199. doi: 1

   Insert

   COMPASS (Cps60, Cps50, Cps35, Cps25, Cps15, Flag-Set1, Cps30, Twinstrep-Cps40) (BRE2, SWD1, SET1, SPP1, SWD3, SWD2, SDC1, SHG1 Budding Yeast)
   

   Use

   Tags

   His6-3xFLAG, Twinstrep

   Expression

   Insect

   Mutation

   Set1(762-1080)

   Promoter

   polyhedrin promoter

   Available since

   March 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAG8Ha-EWS_RRM(359-447)

   Plasmid

   #188045

   Purpose

   Expresses His8-EWS_RRM(359-447) with a TEV site for removal of the His8 tag.

   Depositor

   David Libich

   Article

   Selig et al Biopolymers. 2023 May;114(5):e23536. doi

   Insert

   EWSR1 (EWSR1 Human)
   

   Use

   Tags

   His8

   Expression

   Bacterial

   Mutation

   deleted amino acids 1-358, 446-656

   Promoter

   T7

   Available since

   Oct. 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAG8Ha-EWS_RRM-RGG(359-513)

   Plasmid

   #188046

   Purpose

   Expresses His8-EWS_RRM-RGG(359-513) with a TEV site for removal of the His8 tag.

   Depositor

   David Libich

   Article

   Selig et al Biopolymers. 2023 May;114(5):e23536. doi

   Insert

   EWSR1 (EWSR1 Human)
   

   Use

   Tags

   His8

   Expression

   Bacterial

   Mutation

   deleted amino acids 1-358, 514-656

   Promoter

   T7

   Available since

   Oct. 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pEmal10H-MBP-EWSR1-449-513

   Plasmid

   #199439

   Purpose

   Expresses RG2 (the second RG-rich region, 449-513) of EWS

   Depositor

   David Libich

   Article

   Selig et al Biopolymers. 2023 May;114(5):e23536. doi

   Insert

   EWSR1 (EWSR1 Human)
   

   Use

   Tags

   His10 and MBP

   Expression

   Bacterial

   Mutation

   deleted amino acids 1-448, 514-656

   Promoter

   T7

   Available since

   Oct. 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. sgRNA targeting human SLC12A5 gene stop codon

    Plasmid

    #158577

    Purpose

    For generate double-strand DNA break near the stop codon of the human SLC12A5 gene that encodes neuronal chloride transporter KCC2 protein.

    Depositor

    Rudolf Jaenisch

    Article

    Tang et al Sci Transl Med. 2019 Jul 31;11(503). pii

    Insert

    sgRNA targeting human SLC12A5 gene stop codon
    

    Use

    CRISPR

    Tags

    Cas9 and GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. AAV2_hSyn_Aurora_CA_Citrine

    Plasmid

    #98219

    Purpose

    Slow cycling (open for minutes) step-function artificial anion conducting channelrhodopsin (aACR). High light sensitivity. Activation with green light, inactivation max with 625 nm (accelarates closure to ms). Not recommended for in vivo use. Codon optimized for mammalian expression.

    Depositor

    Simon Wiegert

    Article

    Wietek et al Sci Rep. 2017 Nov 2;7(1):14957. doi: 10.

    Insert

    Synthetic construct Aurora_C128A gene
    

    Use

    AAV

    Tags

    Citrine

    Expression

    Mammalian

    Mutation

    V59S, E83N, E90Q, E101S, V117R, E123S, C128A, P24…

    Promoter

    human synapsin

    Available since

    Aug. 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pACEBac1-PSMA1-PSMA2-PSMA3-PSMA4-PSMA5-PSMA6-PSMA7

    Plasmid

    #214138

    Purpose

    Insect cell expression of human 20S CP alpha-subunits

    Depositor

    Brenda Schulman

    Article

    Adolf et al Nat Struct Mol Biol. 2024 Apr 10. doi: 1

    Inserts

    PSMA1 (PSMA1 Human)
    PSMA2 (PSMA2 Human)
    PSMA3 (PSMA3 Human)
    PSMA4 (PSMA4 Human)
    PSMA5 (PSMA5 Human)
    PSMA6 (PSMA6 Human)
    PSMA7 (PSMA7 Human)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    polyhedrin

    Available since

    May 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pcDNA5/FRT/TO-GAlphaz-RLuc8

    Plasmid

    #140978

    Purpose

    Encodes a G alpha subunit (GNAZ) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

    Depositor

    Bryan Roth

    Article

    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

    Insert

    GAlphaz-Rluc8 (GNAZ Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    RLuc8 and flanking SGGGGS linkers have been inser…

    Promoter

    CMV

    Available since

    June 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pcDNA5/FRT/TO-GAlphaoA-RLuc8

    Plasmid

    #140976

    Purpose

    Encodes a G alpha subunit (GNAO1 Isoform Alpha 1) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

    Depositor

    Bryan Roth

    Article

    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

    Insert

    GAlphaoA-RLuc8 (GNAO1 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    RLuc8 and flanking SGGGGS linkers have been inser…

    Promoter

    CMV

    Available since

    June 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pF CAG luc-EGFP-cre puro

    Plasmid

    #67503

    Purpose

    Constitutive expression of a firefly luciferase-enhanced green fluorescent protein-cre recombinase fusion protein in mammalian cells

    Depositor

    Brett Stringer

    Article

    Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.

    Insert

    luciferase-EGFP-cre
    

    Use

    Cre/Lox, Lentiviral, and Luciferase ; Fluorescent…

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Feb. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pcDNA5/FRT/TO-GAlphai3-RLuc8

    Plasmid

    #140975

    Purpose

    Encodes a G alpha subunit (GNAI3) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

    Depositor

    Bryan Roth

    Article

    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

    Insert

    GAlphai3-RLuc8 (GNAI3 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    RLuc8 and flanking SGGGGS linkers have been inser…

    Promoter

    CMV

    Available since

    June 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pcDNA5/FRT/TO-GAlphasL-RLuc8

    Plasmid

    #140981

    Purpose

    Encodes a G alpha subunit (GNAS1) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

    Depositor

    Bryan Roth

    Article

    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

    Insert

    GAlphasL-RLuc8 (GNAS Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    RLuc8 and flanking SGGGGS linkers have been inser…

    Promoter

    CMV

    Available since

    June 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDNA5/FRT/TO-GAlpha11-RLuc8

    Plasmid

    #140983

    Purpose

    Encodes a G alpha subunit (GNA11) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

    Depositor

    Bryan Roth

    Article

    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

    Insert

    GAlpha11-RLuc8 (GNA11 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    RLuc8 and flanking SGGGGS linkers have been inser…

    Promoter

    CMV

    Available since

    June 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pACEBac1-PSMB1-PSMB2-PSMB3-PSMB4-TEV-2xSTII-PSMB5-PSMB6-PSMB7

    Plasmid

    #214140

    Purpose

    Insect cell expression of human 20S CP beta-subunits with a c-terminal twin-strep-tag on beta7

    Depositor

    Brenda Schulman

    Article

    Adolf et al Nat Struct Mol Biol. 2024 Apr 10. doi: 1

    Inserts

    PSMB1 (PSMB1 Human)
    PSMB2 (PSMB2 Human)
    PSMB3 (PSMB3 Human)
    PSMB4 (PSMB4 Human)
    PSMB5 (PSMB5 Human)
    PSMB6 (PSMB6 Human)
    PSMB7 (PSMB7 Human)
    

    Use

    Tags

    2xSTII

    Expression

    Insect

    Mutation

    Promoter

    polyhedrin

    Available since

    May 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA5/FRT/TO-GAlpha13-RLuc8

    Plasmid

    #140986

    Purpose

    Encodes a G alpha subunit (GNA13) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

    Depositor

    Bryan Roth

    Article

    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

    Insert

    GAlpha13-RLuc8 (GNA13 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    RLuc8 and flanking SGGGGS linkers have been inser…

    Promoter

    CMV

    Available since

    June 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge