We narrowed to 4,440 results for: Abo;
-
Plasmid#158965PurposeWild type VanR where the residue in position 165 was mutated from Phenylalanine to Valine. This mutation abolished the response to vanillic acid. The gene is under the control of the TEF1 promoter.DepositorInsertVanR - F165V
ExpressionYeastMutationWild type VanR from Caulobacter crescentus with m…PromoterTEF1Available SinceJan. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
VanR - F165R
Plasmid#158966PurposeWild type VanR where the residue in position 165 was mutated from Phenylalanine to Arginine. This mutation abolished the response to vanillic acid. The gene is under the control of the TEF1 promoter.DepositorInsertVanR - F165R
ExpressionYeastMutationWild type VanR from Caulobacter crescentus with m…PromoterTEF1Available SinceJan. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pQP-2376
Plasmid#138974PurposeLight-induced recruitment of NSImb-QPAS1-mCherry to the target protein bound to membrane; interaction occurs via intrabody fused to BphP1DepositorInsertNcoI-mVenus-CAAX-IRES2-BphP1- iB(GFP)- IRES2-NSImb-NES-mCh-Q-PAS1-NotI
Available SinceApril 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDN-D2irTNG5kwh
Plasmid#128279PurposeMammalian linearizer gene circuit in a Flp-In expression system with a hTetR::EGFP fusion proteinDepositorInserthTetR::NLS::EGFP
UseSynthetic Biology; Flp-in expression vectorTagsEGFPExpressionMammalianPromoterCMV-D2ir promoterAvailable SinceJan. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pVL37 - pWOX4
Plasmid#116024Purposeentry vector for GreenGate cloning method, promoterDepositorInsertpWOX4
UseUnspecifiedMutationT1473A by SDM to abolish Eco31I siteAvailable SinceNov. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pVL27 - pPXY
Plasmid#116017Purposeentry vector for GreenGate cloning method, promoterDepositorInsertpPXY
UseUnspecifiedMutationT2139A by SDM to abolish Eco31I siteAvailable SinceNov. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMB1-spect-PthrC3_25-eGFP
Plasmid#107413PurposeThis plasmid contain promoter PthrC3_25, which is used for study about Short, Auto-inducible Promoters for Well-Controlled Protein Expression in Escherichia coli.DepositorInserteGFP
ExpressionBacterialPromoterPthrC3_25Available SinceMay 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMB1-spect-PthrC3_7-eGFP
Plasmid#107412PurposeThis plasmid contain promoter PthrC3_7, which is used for study about Short, Auto-inducible Promoters for Well-Controlled Protein Expression in Escherichia coli.DepositorInserteGFP
ExpressionBacterialPromoterPthrC3_7Available SinceMay 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
nucHCB1
Plasmid#97424PurposeNuclear Huntingtin Chromobody 1: "Happ1" intrabody recognizing amino acids 41–81 of huntingtin, fused to eYFP and nuclear localization signal.DepositorInsertnucHCB1
TagsNLS and YFPExpressionMammalianPromoterCMVAvailable SinceJuly 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRSETC-PITPbeta (splice variant 1) K61A
Plasmid#58271Purposebacterial expression of PITP beta K61A mutant (phosphatidylinositol transfer protein beta)DepositorInsertPITP beta (PITPNB Human)
TagsHISExpressionBacterialMutationK61A (abolishes PI binding)PromoterT7Available SinceJuly 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
PX458_3XHA_dFnCas9_KRAB_T2A_eGFP
Plasmid#200389Purpose3XHA tagged dead Cas9 from F. novicida with KRAB repressor domain, T2A self-cleaving peptide and enhanced GFP biomarker for mammalian expressionDepositorInsertPX458_3XHA_dFnCas9_KRAB_eGFP
UseCRISPRTags3xHA-NLS and SV40NLS-KRAB-T2A-EGFPExpressionMammalianPromoterCBHAvailable SinceSept. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFB-U6-control-gRNA-hSyn-mCherry-WPRE-SV40pA
Plasmid#128347PurposepAAV encoding control gRNA for CRISPR gRNAs listed aboveDepositorInsertcontrol (negative)
UseCRISPRExpressionMammalianAvailable SinceJuly 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTRE3G-EGFP-IRES(Neo)R
Plasmid#158068PurposeeGFP under the TRE3G promoter, Neo resistance. For cloning GOIDepositorInserteGFP
TagseGFPExpressionMammalianPromoterTRE3GAvailable SinceAug. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-VSVG
Plasmid#11912DepositorInsertVSVG
ExpressionMammalianMutationF64L, S65T, A206K relative the wild type GFP sequ…Available SinceSept. 13, 2006AvailabilityAcademic Institutions and Nonprofits only -
Laconic/pcDNA3.1(-)
Plasmid#44238Purposea genetically-encoded sensor for lactate concentration, transport and metabolic fluxDepositorInsertLaconic (LACtate Optical Nano Indicator from CECs)
UseLactate biosensorExpressionMammalianPromoterCMVAvailable SinceMay 24, 2013AvailabilityAcademic Institutions and Nonprofits only -
RPL13-ECFP-SNAP
Plasmid#187967Purposeexpresses RPL13 with C-terminal tags ECFP and SNAPDepositorAvailable SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pQE60 R196A wgMDH
Plasmid#204271PurposeBacterial expression of R196A wgMDH with IPTG induction - Active site mutantInsertMDHG_CITLA
Tags6X HisExpressionBacterialMutationR196AAvailable SinceOct. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV_SynP_DIO_tAKARα- T391A_WPRE
Plasmid#196676PurposeAAV plasmid for mammalian Cre-dependent expression of the T391A mutant of tAKARα (abolished sensor phosphorylation site).DepositorInserttAKARα-T391A
UseAAV and Cre/LoxExpressionMammalianAvailable SinceFeb. 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
Ydf NanoLuc
Plasmid#225656PurposeReporter plasmid expressing NanoLuciferase under the control of the promoter PydfJ115, cognate to YdfI. Responds to NarL/NarX chimeras from this study.DepositorInsertNanoLuciferase
UseCell free expressionTagsMycExpressionBacterialPromoterPydfJ115Available SinceDec. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJL NarX Myc
Plasmid#225651PurposeExpresses WT NarX capable of sensing nitrate. For use with NarL-YdfI and reporter plasmids from this study.DepositorAvailable SinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only