Addgene

We narrowed to 4,639 results for: Drosophila

Showing: 4041 - 4060 of 4639 results

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pExpreS2-1-C3-PA-10H-SIII

Plasmid

#175443

Purpose

HiFi-compatible destination vector for secreted overexpression of 3C-cleavable, C-terminal Protein A-10His-TwinStrep tagged proteins with ExpreS2 Platform

Depositor

Wyatt Yue

Article

Coker et al Front Bioeng Biotechnol. 2022 May 5;10:8

Type

Empty backbone

Use

Tags

3C-Protein A-10His-TwinStrep and BiP Secretion Pe…

Expression

Insect

Mutation

Promoter

Fused Actin-HSP70

Available since

April 22, 2022

Availability

Academic Institutions and Nonprofits only

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pAW-CD8-Nb127D01-GFP

Plasmid

#171577

Purpose

Expresses GFP-tagged anti-CXCR2 (human) recombinant llama nanobody (Nb127D01-GFP) in fly cell cytomembrane

Depositor

Hidde Ploegh, Norbert Perrimon

Article

Xu et al Elife. 2022 Jan 25;11. pii: 74326. doi:

Insert

CD8-Nb127D01-GFP (CXCR2 Human)

Use

Tags

CD8 and GFP

Expression

Insect

Mutation

Promoter

fly actin5C promoter

Available since

June 30, 2021

Availability

Academic Institutions and Nonprofits only

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pExpreS2-1-C3-10H-SIII

Plasmid

#175444

Purpose

HiFi-compatible destination vector for secreted overexpression of 3C-cleavable, C-terminal 10His-TwinStrep tagged proteins with ExpreS2 Platform

Depositor

Wyatt Yue

Article

Coker et al Front Bioeng Biotechnol. 2022 May 5;10:8

Type

Empty backbone

Use

Tags

3C-10His-TwinStrep and BiP Secretion Peptide

Expression

Insect

Mutation

Promoter

Fused Actin-HSP70

Available since

March 30, 2022

Availability

Academic Institutions and Nonprofits only

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pJFRC-13XLexAoP-FRT-STOP-FRT-myrGFP-2A-KDR::Pest

Plasmid

#217515

Purpose

Encodes a LexAoP controlled and Flp dependent conditional trangene of bicistronic myrGFP and KD Recombinase

Depositor

S. Lawrence Zipursky

Article

Sanfilippo et al Neuron. 2024 Jan 13:S0896-6273(23)00973-

Insert

13XLexAoP-FRT-STOP-FRT-myrGFP-2A-KDR::Pest

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

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pAW-Nb127D01-mCherry

Plasmid

#171573

Purpose

Expresses mCherry-tagged anti-CXCR2 (human) recombinant llama nanobody (Nb127D01-mCherry) in fly cells

Depositor

Hidde Ploegh, Norbert Perrimon

Article

Xu et al Elife. 2022 Jan 25;11. pii: 74326. doi:

Insert

Nb127D01-mCherry (CXCR2 Human)

Use

Tags

mCherry

Expression

Insect

Mutation

Promoter

fly actin5C promoter

Available since

June 29, 2021

Availability

Academic Institutions and Nonprofits only

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pBS-KS-attB2-SA(1)-T2A-3X Gal80-Hsp70

Plasmid

#62952

Purpose

Creating Gal80 lines from MiMIC lines with inserts into coding introns in Phase 1. Gal80 is tagged with FLAG, HA and EGFP.

Depositor

Benjamin White

Article

Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

Insert

3XGal80-FLAG/HA/EGFP-Hsp70

Use

Tags

Expression

Mutation

Promoter

Available since

July 2, 2015

Availability

Academic Institutions and Nonprofits only

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RpH-ILV-FRB

Plasmid

#225137

Purpose

RpH-ILV probe strongly colocalises with lysosomal markers with FRB

Depositor

Massimiliano Stagi

Article

Cheetham-Wilkinson et al Sci Adv. 2025 Jan 3;11(1):eadr7325. doi:

Insert

TMEM192 (TMEM192 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pDropIn-T2A-split-intein-Gal4[1-20]-N-int-BlastR

Plasmid

#199197

Purpose

Insert to create split-intein Gal4[N-int] knock-in vectors, with blasticidin resistance, via Drop-In cloning, released via BsmBI digest

Depositor

Norbert Perrimon

Article

Ewen-Campen et al Proc Natl Acad Sci U S A. 2023 Jun 13;12

Insert

T2A-split-intein-Gal4[1-20]-N-int-BlastR

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Aug. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pGP-20XUAS-IVS-Syn21-RSET-jGCaMP8s-p10

Plasmid

#218864

Purpose

Gal4 activated transgene expression of ultrafast protein calcium sensor under the hsp70 promoter in D. melanogaster

Depositor

GENIE Project

Article

Reagents et al figshare Dataset. 2024. doi: 10.6084/m9.

Insert

RSET-jGCaMP8s

Use

Tags

6xHis-T7 gene 10 leader-Xpress epitope-Enterokina…

Expression

Insect

Mutation

S26M F286Y Q315H

Promoter

hsp70

Available since

May 14, 2024

Availability

Academic Institutions and Nonprofits only

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pHD-T2A-split-intein-Gal4[1-20]-N-int

Plasmid

#199203

Purpose

To create "long homology arm" (~1000bp) knock-in donor vectors for split-intein Gal4[1-20]N-int

Depositor

Norbert Perrimon

Article

Ewen-Campen et al Proc Natl Acad Sci U S A. 2023 Jun 13;12

Insert

T2A-split-intein-Gal4[1-20]-N-int

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Aug. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pHD-T2A-split-intein-Gal4[21-881]-C-int

Plasmid

#199204

Purpose

To create "long homology arm" (~1000bp) knock-in donor vectors for split-intein Gal4[21-881]C-int

Depositor

Norbert Perrimon

Article

Ewen-Campen et al Proc Natl Acad Sci U S A. 2023 Jun 13;12

Insert

T2A-split-intein-Gal4[21-881]-C-int

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Aug. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pBS-KS-attB2-SA(0)-T2A-LexA::GADfluw-Hsp70

Plasmid

#78304

Purpose

Creating LexA::GADfluw lines from MiMIC lines with inserts into coding introns in Phase 0

Depositor

Benjamin White

Article

Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

Insert

T2A-nlsLexA::GADfluw-Hsp70

Use

Tags

Expression

Mutation

Promoter

Available since

June 2, 2016

Availability

Academic Institutions and Nonprofits only

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pDropIn-T2A-split-intein-Gal4[1-20]-N-int

Plasmid

#199195

Purpose

Insert to create split-intein Gal4[N-int] knock-in vectors via Drop-In cloning, released via BsmBI digest

Depositor

Norbert Perrimon

Article

Ewen-Campen et al Proc Natl Acad Sci U S A. 2023 Jun 13;12

Insert

T2A-split-intein-Gal4[1-20]-N-int, 3xP3-dsRed

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Aug. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pDropIn-T2A-split-intein-Gal4[21-881]-C-int

Plasmid

#199196

Purpose

Insert to create split-intein Gal4[C-int] knock-in vectors via Drop-In cloning, released via BsmBI digest

Depositor

Norbert Perrimon

Article

Ewen-Campen et al Proc Natl Acad Sci U S A. 2023 Jun 13;12

Insert

T2A-split-intein-Gal4[21-881]-C-int, 3xP3-dsRed

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Aug. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pET-26b-NbVHH05-HA-His

Plasmid

#171567

Purpose

Bacterial expression of HA-His-tagged anti-UBC6e (human) recombinant alpaca nanobody (NbVHH05-HA-His)

Depositor

Hidde Ploegh, Norbert Perrimon

Article

Xu et al Elife. 2022 Jan 25;11. pii: 74326. doi:

Insert

NbVHH05-HA-His (UBE2J1 Human)

Use

Tags

HA-tag/His-tag and PelB signal peptide

Expression

Bacterial

Mutation

Promoter

Available since

June 30, 2021

Availability

Academic Institutions and Nonprofits only

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pGP-JFRC59-13XLexAop2-IVS-Syn21-RSET-jGCaMP8f-p10

Plasmid

#218867

Purpose

LexA activated transgene expression of ultrafast protein calcium sensor under the hsp70 promoter in D. melanogaster

Depositor

GENIE Project

Article

Reagents et al figshare Dataset. 2024. doi: 10.6084/m9.

Insert

RSET-jGCaMP8f

Use

Tags

6xHis-T7 gene 10 leader-Xpress epitope-Enterokina…

Expression

Insect

Mutation

Q315L

Promoter

hsp70

Available since

May 22, 2024

Availability

Academic Institutions and Nonprofits only

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pScarlessHD-C-3xVHH05-DsRed

Plasmid

#171580

Purpose

For scarless insertion of 3xVHH05 NanoTag into C-term; Marked by the visible marker 3xP3-DsRed flanked by piggyBac termini.

Depositor

Hidde Ploegh, Norbert Perrimon

Article

Xu et al Elife. 2022 Jan 25;11. pii: 74326. doi:

Insert

3xVHH05 (UBE2J1 Human)

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

June 30, 2021

Availability

Academic Institutions and Nonprofits only

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pScarlessHD-C-3x127D01-DsRed

Plasmid

#171578

Purpose

For scarless insertion of 3x127D01 NanoTag into C-term; Marked by the visible marker 3xP3-DsRed flanked by piggyBac termini.

Depositor

Hidde Ploegh, Norbert Perrimon

Article

Xu et al Elife. 2022 Jan 25;11. pii: 74326. doi:

Insert

3x127D01 (CXCR2 Human)

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

June 30, 2021

Availability

Academic Institutions and Nonprofits only

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pUCIDT-attL1-Human ABeta-attR5

Plasmid

#160436

Purpose

Entry vector for cloning human Amyloid Beta(1-42) using 2-fragment gateway recombination

Depositor

Nicholas Tolwinski

Article

Kaur et al Bio Protoc. 2020 Dec 5;10(23):e3856. doi

Insert

Homo sapiens amyloid beta precursor protein (APP Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pScarlessHD-N-3xVHH05-DsRed

Plasmid

#171581

Purpose

For scarless insertion of 3xVHH05 NanoTag into N-term; Marked by the visible marker 3xP3-DsRed flanked by piggyBac termini.

Depositor

Hidde Ploegh, Norbert Perrimon

Article

Xu et al Elife. 2022 Jan 25;11. pii: 74326. doi:

Insert

3xVHH05 (UBE2J1 Human)

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

June 30, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge