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pHAGE-dCas9

Plasmid

#248566

Purpose

A CRISPR-based technology to study chromosome-specific aneuploidy by targeting human centromeres

Depositor

Teresa Davoli

Article

Bosco et al Cell. 2023 Apr 27;186(9):1985-2001.e19.

Insert

dCas9

Use

CRISPR

Tags

HA, 2xNLS

Expression

Mammalian

Mutation

D10A, H840A

Promoter

CMV, TetO2

Available Since

Nov. 18, 2025

Availability

Academic Institutions and Nonprofits only

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TRE-dCas9(sa)-VP64-AAV

Plasmid

#213035

Purpose

dCas9-VP64 expressed under control of TRE

Depositor

Nadav Ahituv

Article

Nguyen et al Nat Biotechnol. 2025 Feb 4:10.1038/s4158

Insert

dCas9

Use

AAV and CRISPR

Tags

NLS and NLS-VP64

Promoter

TRE, miniCMV

Available Since

Oct. 1, 2025

Availability

Academic Institutions and Nonprofits only

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FLAG-GRP78-No UTRs Wild-Type (pcDNA3.1)

Plasmid

#238429

Purpose

Expresses GRP78 Full Length without UTRs fused to the FLAG Tag

Depositor

Amy Shiu Lee

Article

Tseng et al Neoplasia. 2019 Aug;21(8):837-848. doi:

Insert

78-kDa glucose-regulated protein (HSPA5 Human)

Tags

FLAG

Expression

Mammalian

Mutation

The UTRs of the gene have been deleted.

Available Since

June 30, 2025

Availability

Academic Institutions and Nonprofits only

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MEK1C218C222

Plasmid

#164640

Purpose

Bacterial expression plasmid for His6-MEK1C218C222

Depositor

Ben Davis

Article

Lai et al Cell. 2021 Apr 27. pii: S0092-8674(21)00

Insert

mitogen-activated protein kinase kinase 1 (MAP2K1 Human)

Tags

His6-tag

Expression

Bacterial

Mutation

G(-19)F/S218C/S222C/C277S/C376S

Promoter

T7

Available Since

May 5, 2021

Availability

Academic Institutions and Nonprofits only

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Pet22b-mEGFP-YAP-C-IDR

Plasmid

#166441

Purpose

Bacterial expression of 6x His tagged mEGFP-YAP1-C-IDR fusion protein

Depositor

Shuguo Sun

Article

Yu et al Mol Cell. 2021 Feb 13. pii: S1097-2765(2

Insert

mEGFP, YAP-C-IDR(266-504) (YAP1 Human)

Expression

Bacterial

Available Since

April 7, 2021

Availability

Academic Institutions and Nonprofits only

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pHAGE-neo-YAP-deltaCC

Plasmid

#166469

Purpose

Plasmid coding for YAP-deltaCC (delta298-359) and neomycin resistance in a lentiviral backbone.

Depositor

Shuguo Sun

Article

Yu et al Mol Cell. 2021 Feb 13. pii: S1097-2765(2

Insert

YAP-deltaCC, Neo (YAP1 Human)

Use

Lentiviral

Available Since

April 7, 2021

Availability

Academic Institutions and Nonprofits only

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pHAGE-SIRPB1-R240C

Plasmid

#116680

Purpose

Lentiviral expression of SIRPB1 R240C

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

SIRPB1 (SIRPB1 Human)

Use

Lentiviral

Mutation

R240C

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-SIRPB1-W183*

Plasmid

#116681

Purpose

Lentiviral expression of SIRPB1 W183*

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

SIRPB1 (SIRPB1 Human)

Use

Lentiviral

Mutation

W183*

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-SMAD4-K436N

Plasmid

#116682

Purpose

Lentiviral expression of SMAD4 K436N

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

SMAD4 (SMAD4 Human)

Use

Lentiviral

Mutation

K436N

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-STK19-D89N

Plasmid

#116688

Purpose

Lentiviral expression of STK19 D89N

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

STK19 (STK19 Human)

Use

Lentiviral

Mutation

D89N

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-SIRPB1-D198N

Plasmid

#116676

Purpose

Lentiviral expression of SIRPB1 D198N

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

SIRPB1 (SIRPB1 Human)

Use

Lentiviral

Mutation

D198N

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-SIRPB1-E170K

Plasmid

#116677

Purpose

Lentiviral expression of SIRPB1 E170K

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

SIRPB1 (SIRPB1 Human)

Use

Lentiviral

Mutation

E170K

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-SIRPB1-G290E

Plasmid

#116678

Purpose

Lentiviral expression of SIRPB1 G290E

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

SIRPB1 (SIRPB1 Human)

Use

Lentiviral

Mutation

G290E

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-SIRPB1-R124Q

Plasmid

#116679

Purpose

Lentiviral expression of SIRPB1 R124Q

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

SIRPB1 (SIRPB1 Human)

Use

Lentiviral

Mutation

R124Q

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-PTEN-R233*

Plasmid

#116633

Purpose

Lentiviral expression of PTEN R233*

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

PTEN (PTEN Human)

Use

Lentiviral

Mutation

R233*

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-PTEN-R335*

Plasmid

#116634

Purpose

Lentiviral expression of PTEN R335*

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

PTEN (PTEN Human)

Use

Lentiviral

Mutation

R335*

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-PTEN-S170N

Plasmid

#116635

Purpose

Lentiviral expression of PTEN S170N

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

PTEN (PTEN Human)

Use

Lentiviral

Mutation

S170N

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-PTEN-V166Sfs*14

Plasmid

#116636

Purpose

Lentiviral expression of PTEN V166Sfs*14

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

PTEN (PTEN Human)

Use

Lentiviral

Mutation

V166Sfs*14

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-PTEN-Y27D

Plasmid

#116637

Purpose

Lentiviral expression of PTEN Y27D

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

PTEN (PTEN Human)

Use

Lentiviral

Mutation

Y27D

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-PTEN-L247_P248delLP

Plasmid

#116624

Purpose

Lentiviral expression of PTEN L247_P248delLP

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

PTEN (PTEN Human)

Use

Lentiviral

Mutation

L247_P248delLP

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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