We narrowed to 14,102 results for: GAN

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pML624_RAB1A-N-term_XTEN80-mEGFP-3xHA_pTMPLT

Plasmid

#227906

Purpose

Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

Depositor

Manuel Leonetti

Article

Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

Insert

RAB1A homology arms with XTEN80-mEGFP-3xHA for insertion at N terminus

Use

CRISPR

Available Since

April 10, 2025

Availability

Academic Institutions and Nonprofits only

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pML343_RAB14-N-term_XTEN80-mEGFP-3xHA_pTMPLT

Plasmid

#227908

Purpose

Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

Depositor

Manuel Leonetti

Article

Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

Insert

RAB14 homology arms with XTEN80-mEGFP-3xHA for insertion at N terminus

Use

CRISPR

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pML325_GPR107-C-term_XTEN80-mEGFP-3xHA_pTMPLT

Plasmid

#227909

Purpose

Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

Depositor

Manuel Leonetti

Article

Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

Insert

GPR107 homology arms with XTEN80-mEGFP-3xHA for insertion at C terminus

Use

CRISPR

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pML494_ATP1B3-C-term_XTEN80-sfGFP-3xHA_pTMPLT

Plasmid

#227912

Purpose

Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

Depositor

Manuel Leonetti

Article

Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

Insert

ATP1B3 homology arms with XTEN80-sfGFP-3xHA for insertion at C terminus

Use

CRISPR

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pML359_CAPRIN1-N-term_XTEN80-mEGFP-3xHA_pTMPLT

Plasmid

#227916

Purpose

Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

Depositor

Manuel Leonetti

Article

Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

Insert

CAPRIN1 homology arms with XTEN80-mEGFP-3xHA for insertion at N terminus

Use

CRISPR

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pML346_VPS35-C-term_XTEN80-mEGFP-3xHA_pTMPLT

Plasmid

#227891

Purpose

Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

Depositor

Manuel Leonetti

Article

Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

Insert

VPS35 homology arms with mEGFP-3xHA for insertion at C terminus

Use

CRISPR

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pML348_ATG101-N-term_XTEN80-mEGFP-3xHA_pTMPLT

Plasmid

#227900

Purpose

Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

Depositor

Manuel Leonetti

Article

Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

Insert

ATG101 homology arms with XTEN80-mEGFP-3xHA for insertion at N terminus

Use

CRISPR

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pML460_MAP1LC3B-N-term_XTEN80-mEGFP-3xHA_pTMPLT

Plasmid

#227901

Purpose

Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

Depositor

Manuel Leonetti

Article

Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

Insert

MAP1LC3B homology arms with XTEN80-mEGFP-3xHA for insertion at N terminus

Use

CRISPR

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pML317_COPE-C-term_XTEN80-mEGFP-3xHA_pTMPLT

Plasmid

#227905

Purpose

Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

Depositor

Manuel Leonetti

Article

Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

Insert

COPE homology arms with XTEN80-mEGFP-3xHA for insertion at C terminus

Use

CRISPR

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pcdna6.2- myc ULK1 k46I

Plasmid

#27630

Depositor

Reuben Shaw

Article

Egan et al Science. 2011 Jan 28;331(6016):456-61. d

Insert

mouse myc ULK1 K46I (Myc Mouse)

Tags

myc

Expression

Mammalian

Mutation

Initial M from ULK1 removed. Silent mutation at A…

Available Since

Feb. 18, 2011

Availability

Academic Institutions and Nonprofits only
pTetOne-FLAG-human cGASdeltaN-HA

Plasmid

#186899

Purpose

Doxycycline-dependent expression of human cGAS gene in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Mosallanejad et al Sci Immunol. 2023 Jan 20;8(79):eabp9765.

Insert

Human cGAS truncation mutant (160-522 a.a.) with N-terminal FLAG and C-terminal HA tag (Adamts1 Synthetic, Human)

Use

Retroviral

Tags

FLAG and HA

Mutation

N-terminal truncation

Promoter

TRE3G

Available Since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pRetroX-TRE3G-IRSp53-T2A-Cdc42(G12V)

Plasmid

#221329

Purpose

Dox-inducible expression of FLAG-IRSp53-T2A-HA-Cdc42 G12V in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

Insert

FLAG-IRSp53 and HA-Cdc42(G12V) (Baiap2, Cdc42 Mouse)

Use

Retroviral

Mutation

G12V

Promoter

TRE3G

Available Since

July 16, 2024

Availability

Academic Institutions and Nonprofits only

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pcdna6.2- myc ULK1 4SA

Plasmid

#27631

Depositor

Reuben Shaw

Article

Egan et al Science. 2011 Jan 28;331(6016):456-61. d

Insert

mouse myc ULK1 4SA (Myc Mouse)

Tags

myc

Expression

Mammalian

Mutation

S467A, S555A, T574A, S637A. Initial M from ULK1 r…

Available Since

March 16, 2011

Availability

Academic Institutions and Nonprofits only

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pQCXIN- myc ULK1 wt

Plasmid

#27626

Depositor

Reuben Shaw

Article

Egan et al Science. 2011 Jan 28;331(6016):456-61. d

Insert

mouse myc ULK1 (Myc Mouse)

Use

Retroviral

Tags

myc

Mutation

Initial M from ULK1 removed. Silent mutation at A…

Available Since

April 15, 2011

Availability

Academic Institutions and Nonprofits only

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pENTR1A-Yap S127A

Plasmid

#46050

Depositor

Xin Chen

Article

Tschaharganeh et al Gastroenterology. 2013 Jun;144(7):1530-1

Insert

Yes-kinase associated protein (YAP1 Human)

Use

Entry vector

Tags

2X Flag tag

Mutation

S127A

Available Since

July 2, 2013

Availability

Academic Institutions and Nonprofits only
pQCXIN- myc ULK1 4SA

Plasmid

#27628

Depositor

Reuben Shaw

Article

Egan et al Science. 2011 Jan 28;331(6016):456-61. d

Insert

mouse myc ULK1 4SA (Myc Mouse)

Use

Retroviral

Tags

myc

Mutation

S467A, S555A, T574A, S637A. Initial M from ULK1 r…

Available Since

May 23, 2011

Availability

Academic Institutions and Nonprofits only
pcDNA3-FLAG_FBXL7 (R310H)

Plasmid

#171849

Purpose

pcDNA3-FLAG_FBXL7 (R310H)

Depositor

Michele Pagano

Article

Moro et al Nat Cell Biol. 2020 Sep;22(9):1130-1142.

Insert

F-box and leucine rich repeat protein 7 (FBXL7 Human)

Tags

Flag

Expression

Mammalian

Mutation

changed Arginine 310 to Histidine

Available Since

Nov. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pQCXIN- myc ULK1 K46I

Plasmid

#27627

Depositor

Reuben Shaw

Article

Egan et al Science. 2011 Jan 28;331(6016):456-61. d

Insert

mouse myc ULK1 K46I (Myc Mouse)

Use

Retroviral

Tags

myc

Mutation

Initial M from ULK1 removed. Silent mutation at A…

Available Since

April 15, 2011

Availability

Academic Institutions and Nonprofits only

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pCDNA3 ATP5O(5’UTR)-SL-FF

Plasmid

#85491

Purpose

Firefly luciferase under the control of ATP5O 5'UTR followed by a stem-loop

Depositor

Ivan Topisirovic

Article

Gandin et al Genome Res. 2016 May;26(5):636-48. doi:

Insert

5'UTR of ATP5O followed by stem loop (ATP5PO Human)

Use

Luciferase

Tags

Firefly luciferase

Expression

Mammalian

Available Since

Jan. 13, 2017

Availability

Academic Institutions and Nonprofits only

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pCDNA3 ATP5O(TISU)-FF

Plasmid

#85489

Purpose

Firefly luciferase under the control of ATP5O TISU

Depositor

Ivan Topisirovic

Article

Gandin et al Genome Res. 2016 May;26(5):636-48. doi:

Insert

Full TISU element of ATP5O (ATP5PO Human)

Use

Luciferase

Tags

Firefly luciferase

Expression

Mammalian

Mutation

2nd and 3rd codon of luciferase were modified fro…

Available Since

Jan. 4, 2017

Availability

Academic Institutions and Nonprofits only

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