We narrowed to 5,553 results for: crispr cas9 grna plasmid

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RIF C-terminal sgRNA

Plasmid

#207096

Purpose

pX330 based plasmid for expression of Cas9 and the AATACTAAATAGAATTTTCA sgRNA to target the RIF1 locus.

Depositor

Jens Schmidt

Article

Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

Insert

AATACTAAATAGAATTTTCA

Expression

Mammalian

Promoter

CMV and U6

Available Since

May 1, 2025

Availability

Academic Institutions and Nonprofits only

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RNF168 C-terminal sgRNA

Plasmid

#207100

Purpose

pX330 based plasmid for expression of Cas9 and the GAGATGCACAAAGTAAGGCC sgRNA to target the RNF168 locus.

Depositor

Jens Schmidt

Article

Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

Insert

GAGATGCACAAAGTAAGGCC

Expression

Mammalian

Promoter

CMV and U6

Available Since

May 1, 2025

Availability

Academic Institutions and Nonprofits only

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ATM N-terminal sgRNA

Plasmid

#207089

Purpose

pX330 based plasmid for expression of Cas9 and the ATCATTAAGTACTAGACTCA sgRNA to target the ATM locus.

Depositor

Jens Schmidt

Article

Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

Insert

ATCATTAAGTACTAGACTCA

Expression

Mammalian

Promoter

CMV and U6

Available Since

April 30, 2024

Availability

Academic Institutions and Nonprofits only

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REV7 C-terminal sgRNA

Plasmid

#207095

Purpose

pX330 based plasmid for expression of Cas9 and the GCTCATAAAGGCAGCTGAGG sgRNA to target the REV7 locus.

Depositor

Jens Schmidt

Article

Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

Insert

GCTCATAAAGGCAGCTGAGG

Expression

Mammalian

Promoter

CMV and U6

Available Since

May 1, 2025

Availability

Academic Institutions and Nonprofits only

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SHLD3 N-terminal sgRNA

Plasmid

#207094

Purpose

pX330 based plasmid for expression of Cas9 and the TTACTGCAGAATGACTACAG sgRNA to target the SHLD3 locus.

Depositor

Jens Schmidt

Article

Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

Insert

TTACTGCAGAATGACTACAG

Expression

Mammalian

Promoter

CMV and U6

Available Since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

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NBS1 C-terminal sgRNA

Plasmid

#207092

Purpose

pX330 based plasmid for expression of Cas9 and the TAAAAAGGAGAAGATAACTG sgRNA to target the NBS1 locus.

Depositor

Jens Schmidt

Article

Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

Insert

TAAAAAGGAGAAGATAACTG

Expression

Mammalian

Promoter

CMV and U6

Available Since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

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RNF169 C-terminal sgRNA

Plasmid

#207099

Purpose

pX330 based plasmid for expression of Cas9 and the ACACTTCATTAGGTGCTACT sgRNA to target the RNF169 locus.

Depositor

Jens Schmidt

Article

Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

Insert

ACACTTCATTAGGTGCTACT

Expression

Mammalian

Promoter

CMV and U6

Available Since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

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SHLD1 N-terminal sgRNA

Plasmid

#207101

Purpose

pX330 based plasmid for expression of Cas9 and the ATGGCAGGACTATGGCAGCC sgRNA to target the SHLD1 locus.

Depositor

Jens Schmidt

Article

Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

Insert

ATGGCAGGACTATGGCAGCC

Expression

Mammalian

Promoter

CMV and U6

Available Since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

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ATM C-terminal sgRNA

Plasmid

#207097

Purpose

pX330 based plasmid for expression of Cas9 and the TTTCTAAAGGCTGAATGAAA sgRNA to target the ATM locus.

Depositor

Jens Schmidt

Article

Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

Insert

TTTCTAAAGGCTGAATGAAA

Expression

Mammalian

Promoter

CMV and U6

Available Since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

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SHLD2 N-terminal sgRNA

Plasmid

#207098

Purpose

pX330 based plasmid for expression of Cas9 and the TTTTTATCAGAAATCATGAG sgRNA to target the SHLD2 locus.

Depositor

Jens Schmidt

Article

Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

Insert

TTTTTATCAGAAATCATGAG

Expression

Mammalian

Promoter

CMV and U6

Available Since

April 10, 2024

Availability

Academic Institutions and Nonprofits only

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MDC1 N-terminal sgRNA

Plasmid

#207090

Purpose

pX330 based plasmid for expression of Cas9 and the GTATCCTTCCCAGATCATGG sgRNA to target the MDC1 locus.

Depositor

Jens Schmidt

Article

Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

Insert

GTATCCTTCCCAGATCATGG

Expression

Mammalian

Promoter

CMV and U6

Available Since

March 28, 2024

Availability

Academic Institutions and Nonprofits only

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pSEVA47_dCas9-GFP_AAN_4

Plasmid

#231415

Purpose

This AND-AND-NOT-gate plasmid expresses dCas9 from a constitutive promoter. Its sgRNAs X & Y are repressible by sgRNAs A & B, respectively. Its GFP gene is repressible by any of sgRNAs X, Y, and C.

Depositor

Manish Kushwaha

Article

Pujar et al Nucleic Acids Res. 2024 Dec 27:gkae1256.

Inserts

dCas9
GFP
sgRNA-X
sgRNA-Y

Use

Synthetic Biology

Available Since

Feb. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV2ss-U6-sgHTT1-7sk-sgCas9

Plasmid

#190900

Purpose

AAV-KamiCas9 vector expressing sgGFP and human HTT sgRNAs

Depositor

Nicole Déglon

Article

Duarte et al Mol Ther Methods Clin Dev. 2023 Mar 27;2

Insert

sgRNA (HTT Human, spCas9)

Use

AAV and CRISPR

Promoter

U6 and 7sk

Available Since

March 27, 2023

Availability

Academic Institutions and Nonprofits only

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pAAVss-U6-sgHTT51-7sk-Cas9

Plasmid

#190901

Purpose

AAV-KamiCas9 vector expressing sgGFP and mouse HTT sgRNA

Depositor

Nicole Déglon

Article

Duarte et al Mol Ther Methods Clin Dev. 2023 Mar 27;2

Insert

sgRNA (Htt Mouse)

Use

AAV and CRISPR

Promoter

U6 and 7sk

Available Since

March 27, 2023

Availability

Academic Institutions and Nonprofits only

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pCRISPR-PsppA-mCherry

Plasmid

#225428

Purpose

Plasmid encodes SgRNA, SpCas9 and homologous arms for homologous recombination of mCherry

Depositor

Geir Mathiesen

Article

Wiull et al Microbiol Spectr. 2025 Feb 4;13(2):e0202

Insert

mCherry, Cas9, SgRNA

Use

CRISPR

Expression

Bacterial

Promoter

PsppA upstream of mCherry and Cas9, P3 upstream o…

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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scAAV-sgRNA-GFP

Plasmid

#177935

Purpose

Expresses sgRNA and can be packaged into AAV particles for somatic delivery into Cas9 transgenic mice

Depositor

Roland Rad

Article

Kaltenbacher et al Nat Protoc. 2022 Apr;17(4):1142-1188. do

Type

Empty backbone

Use

AAV and CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Nov. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pLenti-CRISPRv2-sgENPP1_#3-hygro

Plasmid

#254680

Purpose

knockout human ENPP1

Depositor

Brian Ruffell

Insert

sgRNA with Cas9 and hygromycin resistance (ENPP1 Human)

Use

CRISPR and Lentiviral

Available Since

May 5, 2026

Availability

Academic Institutions and Nonprofits only

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pLenti-CRISPRv2-sgENPP1_#2-hygro

Plasmid

#254679

Purpose

knockout human ENPP1

Depositor

Brian Ruffell

Insert

sgRNA with Cas9 and hygromycin resistance (ENPP1 Human)

Use

CRISPR and Lentiviral

Available Since

May 5, 2026

Availability

Academic Institutions and Nonprofits only

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pLenti-CRISPRv2-sgENPP1_#1-hygro

Plasmid

#254678

Purpose

knockout human ENPP1

Depositor

Brian Ruffell

Insert

sgRNA with Cas9 and hygromycin resistance (ENPP1 Human)

Use

CRISPR and Lentiviral

Available Since

May 5, 2026

Availability

Academic Institutions and Nonprofits only

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px-458-sgRNA-IF1

Plasmid

#206923

Purpose

Plasmid expressing Cas9, GFP and guides to human IF1 to generate IF1-KO mammalian cell lines

Depositor

José Cuezva

Article

Romero-Carraminana et al Commun Biol. 2023 Aug 12;6(1):836. doi:

Insert

sgRNA targeting IF1 (ATPase inhibitory factor 1) (ATP5IF1 Human)

Use

CRISPR

Promoter

U6

Available Since

Jan. 21, 2026

Availability

Academic Institutions and Nonprofits only

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