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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 18,824 results for: nls

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Showing: 4041 - 4060 of 18824 results

  6. pTS1026-Tier1-PhCMV-3xNLS-iRFP670

    Plasmid
    #169532
    Purpose
    Tier-1 vector encoding PhCMV-driven iRFP670 that is localized to the nucleus (PhCMV-3xNLS-iRFP670-pA).
    Depositor
    Martin Fussenegger
    Publication
    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    PCMV-driven nucleus-localized far-red fluorescent protein iRFP670
    Expression
    Mammalian
    Promoter
    PhCMV
    Available since
    June 16, 2022
    Availability
    Academic Institutions and Nonprofits only
    169532-plasmid-map-sequence-id-364613

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  7. pBS-Nvec-codonopti-Cas9-2xNLS

    Plasmid
    #141108
    Purpose
    Plasmid containing insert for the IVT of nuclear localized Cas9 mRNA with codon optimization for Nematostella vectensis
    Depositor
    Matthew Gibson
    Publication
    Hill et al Methods Mol Biol. 2022;2450:437-465. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Nematostella codon optimized Cas9
    Use
    Unspecified
    Mutation
    All amino acids coded for by codons optimized for…
    Promoter
    T7
    Available since
    Oct. 22, 2020
    Availability
    Academic Institutions and Nonprofits only
    141108-plasmid-map-sequence-id-314929

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  8. pCSD_N-SpCas9-1-573-FRB-1xNLS

    Plasmid
    #107298
    Purpose
    Expresses N terminal SpCas9 (1-573 aa) fused to FRB in mammalian cells
    Depositor
    Scot Wolfe
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Truncated SpCas9 (aa 1-573) fused to FRB
    Use
    CRISPR
    Tags
    FRB and SV40 NLS
    Expression
    Mammalian
    Mutation
    Truncated SpCas9 (aa 1-573)
    Promoter
    CMV IE94
    Available since
    Nov. 15, 2018
    Availability
    Academic Institutions and Nonprofits only
    107298-plasmid-map-sequence-id-204614

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  11. ARB365

    Plasmid
    #124048
    Purpose
    Encodes pEF1a driving expression of TET3G P2A iRFP713, pEF1a expressing sadCas9 fused to mRuby2 P2A mRuby2, and an sgRNA targeting pUAS expressing mAzamiGreen in a PiggyBac destination vector
    Depositor
    Hana El-Samad
    Publication
    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    PB_pEF1a-tet3G-P2A-iRFP713_pEF1a-sadCAS9::mRuby2-P2A-mRuby2-SV40PA_mu6-sgUAS_pUAS-NLS::mAzamiGreen-rgPA
    Expression
    Mammalian
    Available since
    Dec. 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    124048-plasmid-map-sequence-id-252519

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  13. pSFV_Nb139-NLS-VP64 (3'UTR)_RLEloop

    Plasmid
    #240256
    Purpose
    Attenuated PROTEUS vector with Nb139-VP64 transgene. Package VLVs with an envelope-expressing vector such as pCMV-VSV-G, or a transgene-responsive VSV-G expression vector.
    Depositor
    Gregory Neely
    Publication
    Cole et al Nat Commun. 2025 May 7;16(1):4250. doi:
    Insert
    nanobody Nb139
    Tags
    NLS-VP64
    Expression
    Mammalian
    Mutation
    VP64 = 4*VP16[aa437-447]
    Promoter
    SFV subgenomic promoter
    Available since
    Feb. 4, 2026
    Availability
    Academic Institutions and Nonprofits only
    240256-plasmid-map-sequence-id-487183

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  17. 1203N-CMV-dDiCas7-11-NLS-bGHpolyA

    Plasmid
    #221537
    Purpose
    Deactivated DiCas7-11 effector with CMV promoter/enhancer and nuclear localization signal sequence at 5' and 3' end of the coding domain
    Depositor
    Omar Akbari
    Insert
    DiCas7-11
    Use
    CRISPR
    Expression
    Mammalian
    Available since
    Feb. 19, 2025
    Availability
    Academic Institutions and Nonprofits only
    221537-plasmid-map-sequence-id-437448

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  18. 1203T-CMV-Cas7.1-NLS-bGHpolyA

    Plasmid
    #221540
    Purpose
    Effector only expresses Cas7.1 domain (truncated from DiCas7-11 effector), nuclear localization signal sequence on 5' and 3' end of the coding domain
    Depositor
    Omar Akbari
    Insert
    Truncated DiCas7-11
    Use
    CRISPR
    Expression
    Mammalian
    Available since
    Feb. 19, 2025
    Availability
    Academic Institutions and Nonprofits only
    221540-plasmid-map-sequence-id-437823

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Showing: 4041 - 4060 of 18824 results