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pAGM1467

Plasmid

#50298

Depositor

Sylvestre Marillonnet, Nicola Patron

Publication

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

5'UTR, omega (Tobacco Mosaic Virus) + HIS tag + enterokinase cleavage site

Use

Plant expression

Mutation

BsaI/BbsI sites removed by point-mutation

Available since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pICH37431

Plasmid

#50306

Depositor

Sylvestre Marillonnet, Nicola Patron

Publication

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

signal peptide, RAmy3A (O. sativa)

Use

Plant expression

Mutation

BsaI/BbsI sites removed by point-mutation

Available since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pICH45089

Plasmid

#50254

Depositor

Sylvestre Marillonnet

Publication

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

promoter (double), 35s (Cauliflower Mosaic Virus)

Use

Plant expression

Mutation

BsaI/BbsI sites removed by point-mutation

Available since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pICH42760

Plasmid

#50257

Depositor

Sylvestre Marillonnet, Nicola Patron

Publication

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

promoter, spm (Z. mays)

Use

Plant expression

Mutation

BsaI/BbsI sites removed by point-mutation

Available since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pICH45131

Plasmid

#50262

Depositor

Sylvestre Marillonnet, Nicola Patron

Publication

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

promoter, LHB1B2 (AT2g34420, A. thaliana)

Use

Plant expression

Mutation

BsaI/BbsI sites removed by point-mutation

Available since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pICH88103

Plasmid

#50273

Depositor

Sylvestre Marillonnet, Nicola Patron

Publication

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

Promoter + 5'UTR, ocs, (A. tumefaciens) + 5'UTR, omega (Tobacco Mosaic Virus)

Use

Plant expression

Mutation

BsaI/BbsI sites removed by point-mutation

Available since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pICH45234

Plasmid

#50276

Depositor

Sylvestre Marillonnet, Nicola Patron

Publication

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

promoter + 5' UTR, RbcS2B (AT5g38430, A. thaliana)

Use

Plant expression

Mutation

BsaI/BbsI sites removed by point-mutation

Available since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pICH45214

Plasmid

#50278

Depositor

Sylvestre Marillonnet, Nicola Patron

Publication

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

promoter + 5' UTR, LHB1B1 (AT2g34430, A. thaliana)

Use

Plant expression

Mutation

BsaI/BbsI sites removed by point-mutation

Available since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pICH87655

Plasmid

#50279

Depositor

Sylvestre Marillonnet, Nicola Patron

Publication

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

promoter, cab2 (AT1g29930, A. thaliana) and 5'UTR, omega (Tobacco Mosaic Virus)

Use

Plant expression

Mutation

BsaI/BbsI sites removed by point-mutation

Available since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pFM110

Plasmid

#40156

Depositor

Geraldine Seydoux

Publication

Motegi et al Nat Cell Biol. 2011 Oct 9;13(11):1361-7.

Insert

par-2 RNAi resistant, 7SE (par-2 Nematode)

Tags

GST

Expression

Bacterial

Mutation

7SE; base pairs 6-523 of par-2 were recoded to c…

Promoter

tac

Available since

Oct. 23, 2012

Availability

Academic Institutions and Nonprofits only
pFM038

Plasmid

#40141

Depositor

Geraldine Seydoux

Publication

Motegi et al Nat Cell Biol. 2011 Oct 9;13(11):1361-7.

Insert

par-2 RNAi resistant (R183-5A + 7SA) (par-2 Nematode)

Use

Gateway Cloning

Tags

GFP

Expression

Worm

Mutation

R183-5A + 7SA; base pairs 6-523 of par-2 were re…

Promoter

pie-1

Available since

Oct. 23, 2012

Availability

Academic Institutions and Nonprofits only
pFM113

Plasmid

#40159

Depositor

Geraldine Seydoux

Publication

Motegi et al Nat Cell Biol. 2011 Oct 9;13(11):1361-7.

Insert

par-3N (par-3, par-3 Nematode)

Tags

GST

Expression

Bacterial

Mutation

N terminal portion of protein (aa1-364)

Promoter

tac

Available since

Oct. 23, 2012

Availability

Academic Institutions and Nonprofits only
pFM114

Plasmid

#40160

Depositor

Geraldine Seydoux

Publication

Motegi et al Nat Cell Biol. 2011 Oct 9;13(11):1361-7.

Insert

par-3N+S251A (par-3 Nematode)

Tags

GST

Expression

Bacterial

Mutation

N terminal portion of protein (aa 1-364); S251A

Promoter

tac

Available since

Oct. 23, 2012

Availability

Academic Institutions and Nonprofits only
pFM107

Plasmid

#40153

Depositor

Geraldine Seydoux

Publication

Motegi et al Nat Cell Biol. 2011 Oct 9;13(11):1361-7.

Insert

par-2 RNAi resistant, 7SA (par-2 Nematode)

Tags

GST

Expression

Bacterial

Mutation

7SA; base pairs 6-523 of par-2 were recoded to c…

Promoter

tac

Available since

Oct. 4, 2012

Availability

Academic Institutions and Nonprofits only
pFM101

Plasmid

#40147

Depositor

Geraldine Seydoux

Publication

Motegi et al Nat Cell Biol. 2011 Oct 9;13(11):1361-7.

Insert

gfp::par-2 wild-type RNAi resistant (par-2 Nematode)

Tags

GST and eGFP

Expression

Bacterial

Mutation

Base pairs 6-523 of par-2 were recoded to create …

Promoter

tac

Available since

Sept. 27, 2012

Availability

Academic Institutions and Nonprofits only
pFM102

Plasmid

#40148

Depositor

Geraldine Seydoux

Publication

Motegi et al Nat Cell Biol. 2011 Oct 9;13(11):1361-7.

Insert

gfp::par-2 RNAi resistant R183-5A (par-2 Nematode)

Tags

GST and eGFP

Expression

Bacterial

Mutation

R183-5A; base pairs 6-523 of par-2 were recoded t…

Promoter

tac

Available since

Sept. 27, 2012

Availability

Academic Institutions and Nonprofits only
pFM103

Plasmid

#40149

Depositor

Geraldine Seydoux

Publication

Motegi et al Nat Cell Biol. 2011 Oct 9;13(11):1361-7.

Insert

par-2 RNAi resistant, wild-type (par-2 Nematode)

Tags

GST

Expression

Bacterial

Mutation

Base pairs 6-523 of par-2 were recoded to create…

Promoter

tac

Available since

Sept. 20, 2012

Availability

Academic Institutions and Nonprofits only
pFM041

Plasmid

#40144

Depositor

Geraldine Seydoux

Publication

Motegi et al Nat Cell Biol. 2011 Oct 9;13(11):1361-7.

Insert

par-2 RNAi resistant (S241A) (par-2 Nematode)

Use

Gateway Cloning

Tags

GFP

Expression

Worm

Mutation

S241A; base pairs 6-523 of par-2 were recoded to…

Promoter

pie-1

Available since

Sept. 20, 2012

Availability

Academic Institutions and Nonprofits only
pFM039

Plasmid

#40142

Depositor

Geraldine Seydoux

Publication

Motegi et al Nat Cell Biol. 2011 Oct 9;13(11):1361-7.

Insert

par-2 RNAi resistant (R183-5A + S241A) (par-2 Nematode)

Use

Gateway Cloning

Tags

GFP

Expression

Worm

Mutation

R183-5A + S241A; base pairs 6-523 of par-2 were …

Promoter

pie-1

Available since

Sept. 20, 2012

Availability

Academic Institutions and Nonprofits only
pFM036

Plasmid

#40139

Depositor

Geraldine Seydoux

Publication

Motegi et al Nat Cell Biol. 2011 Oct 9;13(11):1361-7.

Insert

par-2 RNAi resistant (K162A) (par-2 Nematode)

Use

Gateway Cloning

Tags

GFP

Expression

Worm

Mutation

K162A; base pairs 6-523 of par-2 were recoded to…

Promoter

pie-1

Available since

Sept. 19, 2012

Availability

Academic Institutions and Nonprofits only