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pAc5.1B-EGFP-DmBelle_299-694_B

Plasmid

#145915

Purpose

Insect Expression of DmBelle_299-694

Depositor

Elisa Izaurralde

Insert

DmBelle_299-694 (bel Fly)

Expression

Insect

Available since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmBelle_299-798_B

Plasmid

#145916

Purpose

Insect Expression of DmBelle_299-798

Depositor

Elisa Izaurralde

Insert

DmBelle_299-798 (bel Fly)

Expression

Insect

Available since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmBelle_695-798_B

Plasmid

#145917

Purpose

Insect Expression of DmBelle_695-798

Depositor

Elisa Izaurralde

Insert

DmBelle_695-798 (bel Fly)

Expression

Insect

Available since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pHBDuet139

Plasmid

#121913

Purpose

Charge-Introduced NpuDnaB mini-intein

Depositor

Hideo Iwai

Publication

Beyer et al FEBS J. 2019 Oct 30. doi: 10.1111/febs.1

Insert

CI-NpuDnaBΔ290 intein

Tags

GB1 and His6-GB1

Expression

Bacterial

Mutation

Deletion of 290-residue endonuclease domain, I53K…

Promoter

T7

Available since

Dec. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pZNF57-donor

Plasmid

#112296

Purpose

CRISPR donor plasmid to tag human transcription factor ZNF57 with GFP

Depositor

Kevin White

Insert

ZNF57 homology arms flanking EGFP-IRES-Neo cassette (ZNF57 Human)

Use

CRISPR

Mutation

rs2288959, rs2288958, rs10410539, hg38:19:2918211…

Available since

Dec. 20, 2018

Availability

Academic Institutions and Nonprofits only

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RV-cag-Dio-Kif5c-2A-tdt

Plasmid

#87668

Purpose

retroviral vector, conditional expression membrane Tdtomato and Kif5Cdelta560

Depositor

Oscar Marin

Publication

Lim et al Nat Neurosci. 2018 Jun 18. pii: 10.1038/

Insert

Kif5C-P2A-mTdt (Kif5c Rat)

Use

Retroviral

Tags

palmitylation

Mutation

motor domain aa1-560 present, V40A, E125V (see de…

Promoter

CAG

Available since

April 19, 2017

Availability

Academic Institutions and Nonprofits only

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SNRK gRNA (BRDN0001149393)

Plasmid

#76731

Purpose

3rd generation lentiviral gRNA plasmid targeting human SNRK

Depositor

John Doench, David Root

Publication

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

SNRK (SNRK Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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ACVR1C gRNA (BRDN0001146259)

Plasmid

#76522

Purpose

3rd generation lentiviral gRNA plasmid targeting human ACVR1C

Depositor

John Doench, David Root

Publication

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

ACVR1C (ACVR1C Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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ACVR1C gRNA (BRDN0001149404)

Plasmid

#76523

Purpose

3rd generation lentiviral gRNA plasmid targeting human ACVR1C

Depositor

John Doench, David Root

Publication

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

ACVR1C (ACVR1C Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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ACVR1C gRNA (BRDN0001149510)

Plasmid

#76524

Purpose

3rd generation lentiviral gRNA plasmid targeting human ACVR1C

Depositor

John Doench, David Root

Publication

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

ACVR1C (ACVR1C Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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ACVR1C gRNA (BRDN0001146192)

Plasmid

#76525

Purpose

3rd generation lentiviral gRNA plasmid targeting human ACVR1C

Depositor

John Doench, David Root

Publication

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

ACVR1C (ACVR1C Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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SBK3 gRNA (BRDN0001146759)

Plasmid

#75899

Purpose

3rd generation lentiviral gRNA plasmid targeting human SBK3

Depositor

John Doench, David Root

Publication

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

SBK3 (SBK3 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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SBK3 gRNA (BRDN0001147686)

Plasmid

#75900

Purpose

3rd generation lentiviral gRNA plasmid targeting human SBK3

Depositor

John Doench, David Root

Publication

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

SBK3 (SBK3 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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SBK3 gRNA (BRDN0001148159)

Plasmid

#75901

Purpose

3rd generation lentiviral gRNA plasmid targeting human SBK3

Depositor

John Doench, David Root

Publication

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

SBK3 (SBK3 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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SBK2 gRNA (BRDN0001146871)

Plasmid

#75881

Purpose

3rd generation lentiviral gRNA plasmid targeting human SBK2

Depositor

John Doench, David Root

Publication

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

SBK2 (SBK2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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SBK2 gRNA (BRDN0001147500)

Plasmid

#75882

Purpose

3rd generation lentiviral gRNA plasmid targeting human SBK2

Depositor

John Doench, David Root

Publication

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

SBK2 (SBK2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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SBK2 gRNA (BRDN0001162195)

Plasmid

#75883

Purpose

3rd generation lentiviral gRNA plasmid targeting human SBK2

Depositor

John Doench, David Root

Publication

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

SBK2 (SBK2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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SBK2 gRNA (BRDN0001162192)

Plasmid

#75884

Purpose

3rd generation lentiviral gRNA plasmid targeting human SBK2

Depositor

John Doench, David Root

Publication

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

SBK2 (SBK2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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Sema6d.x-AP-His

Plasmid

#72047

Purpose

Expresses the extracellular region of the Sema6D, isoform x protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

Depositor

Woj Wojtowicz

Publication

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Sema6d.x (Sema6d Human, Mouse)

Tags

AP-His

Expression

Mammalian

Promoter

CMV

Available since

Feb. 18, 2016

Availability

Academic Institutions and Nonprofits only

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pEGFP C1 SADBs shRNA Resistant

Plasmid

#67155

Purpose

pEGFP containing N-terminally EGFP Tagged mouse SADBs resistant to an shRNA

Depositor

Maria Alvarado-Kristensson, Ana Carrera

Publication

Alvarado-Kristensson et al Nat Cell Biol. 2009 Sep;11(9):1081-92. d

Insert

Brsk1 (Brsk1 Mouse)

Tags

EGFP

Expression

Mammalian

Mutation

nucleotide 294 C-T, nucleotide 297 C-G

Promoter

CMV

Available since

July 8, 2015

Availability

Academic Institutions and Nonprofits only