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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 7,765 results for: Pol

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Showing: 4081 - 4100 of 7765 results

  12. pCAG-T3-st1CAS9-pA

    Plasmid
    #131542
    Purpose
    Expresses S. thermophilus 1-derived Cas9 nuclease in mammalian cells. Used to modify genome of mouse embroys
    Depositor
    Wataru Fujii
    Publication
    Fujii et al Biochem Biophys Res Commun. 2016 Jun 16.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Codon optimized st1Cas9
    Use
    CRISPR
    Tags
    Flag, NLS, and polyA
    Expression
    Mammalian
    Available since
    Nov. 5, 2019
    Availability
    Academic Institutions and Nonprofits only
    131542-plasmid-map-sequence-id-262465

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  13. pGEM-myr-tdTomato-SV40pA-FKF

    Plasmid
    #119867
    Purpose
    Donor cassette containing myr-tagged tdTomato and SV40 polyA tail, followed by FRT site-flanked Kanamycin selection gene
    Depositor
    Tatjana Sauka-Spengler
    Publication
    Weinberger et al Dev Cell. 2020 Mar 9;52(5):574-590.e6. d
    Insert
    myr-tagged tdTomato and SV40 polyA, followed by FRT site-flanked Kanamycin selection gene
    Tags
    2x myristoylation
    Expression
    Bacterial
    Available since
    March 28, 2019
    Availability
    Academic Institutions and Nonprofits only
    119867-plasmid-map-sequence-id-239422

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  14. pGEM-myr-eGFP-SV40pA-FKF

    Plasmid
    #119866
    Purpose
    Donor cassette containing myr-tagged eGFP and SV40 polyA tail, followed by FRT site-flanked Kanamycin selection gene
    Depositor
    Tatjana Sauka-Spengler
    Publication
    Weinberger et al Dev Cell. 2020 Mar 9;52(5):574-590.e6. d
    Insert
    myr-tagged eGFP and SV40 polyA, followed by FRT site-flanked Kanamycin selection gene
    Tags
    2x myristoylation
    Expression
    Bacterial
    Available since
    March 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    119866-plasmid-map-sequence-id-239420

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  15. pOCC204

    Plasmid
    #118894
    Purpose
    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal MBP tag, cleavable with 3C and C-terminal mKate2, and HIS6, cleavable with 3C
    Depositor
    Marc Bickle, PEPC MPI CBG
    Publication
    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do
    Insert
    NcoI-MBP-3C-NotI-ccdB-AscI-mKate2-3C-HIS6-stop-HindIII cassette
    Tags
    MBP, cleavable with 3C protease and mKate2, HIS6,…
    Expression
    Insect
    Promoter
    polH
    Available since
    Jan. 3, 2019
    Availability
    Academic Institutions and Nonprofits only
    118894-plasmid-map-sequence-id-232313

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  16. pOCC94

    Plasmid
    #118912
    Purpose
    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with honey bee melitine secretion signal, N-terminal MBP, cleavable with 3C protease, and C-terminal eGFP-HIS6
    Depositor
    Marc Bickle, PEPC MPI CBG
    Publication
    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do
    Insert
    NcoI-HBMss-MBP-3C-NotI-ccdB-AscI-eGFP-HIS6-stop-HindIII cassette
    Tags
    MBP, cleavable with 3C protease and eGFP-HIS6
    Expression
    Insect
    Promoter
    polH
    Available since
    Jan. 2, 2019
    Availability
    Academic Institutions and Nonprofits only
    118912-plasmid-map-sequence-id-232258

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  17. NUDT17 (NUDT17A-c014)

    Plasmid
    #98224
    Purpose
    Baculovirus expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    NUDT17A (NUDT17 Human)
    Use
    Baculovirus
    Tags
    His6-ZBasic-TEV
    Mutation
    pfam00293:NUDIX 93-235
    Promoter
    polyhedrin promoter
    Available since
    Sept. 12, 2017
    Availability
    Academic Institutions and Nonprofits only
    98224-plasmid-map-sequence-id-193301

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  20. pMpGWB414

    Plasmid
    #68679
    Purpose
    Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants
    Depositor
    Takayuki Kohchi
    Publication
    Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do
    Type
    Empty backbone
    Tags
    Glucocorticoid receptor (GR)
    Expression
    Plant
    Promoter
    Cauliflower mosaic virus 35S promoter
    Available since
    June 24, 2016
    Availability
    Academic Institutions and Nonprofits only
    68679-plasmid-map-sequence-id-121733

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Showing: 4081 - 4100 of 7765 results