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We narrowed to 14,352 results for: Cas9

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Showing: 4141 - 4160 of 14352 results

  1. pb-CAGGS-dCas9-VP64-blasto

    Plasmid
    #218556
    Purpose
    Encodes CAG promoter-driven dCas9-VP64 with a bipartite SV40 NLS between dCas9 and VP64, along with blasticidin resistance
    Depositor
    Alejandro Chavez
    Publication
    Giddins et al Nat Commun. 2025 Nov 20;16(1):11114. doi
    Insert
    dCas9
    Use
    CRISPR; Piggybac transposition
    Tags
    2xSV40NLS-VP64
    Expression
    Mammalian
    Mutation
    Cas9 mutations to make dCas9=D10A+D839A+H840A+N86…
    Available since
    Aug. 13, 2025
    Availability
    Academic Institutions and Nonprofits only
    218556-plasmid-map-sequence-id-431934

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  2. pME-dCas9-BFP-KRAB

    Plasmid
    #221502
    Purpose
    CRISPRi machinery fish codon optimized
    Depositor
    Alessandro Ori
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dCas9-tagBFP-KRAB
    Use
    Fish expression
    Mutation
    D10A and H839A point mutations
    Available since
    July 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    221502-plasmid-map-sequence-id-439413

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  5. dCas9-p300 PHD-HAT

    Plasmid
    #179545
    Purpose
    encodes S. pyogenes dCas9 with c-terminal fusion of human p300 PHD-HAT domain (aa 1243-1664) driven by EF-1alpha promoter
    Depositor
    Isaac Hilton
    Publication
    Wang et al Nucleic Acids Res. 2022 Aug 12;50(14):78
    Insert
    S. pyogenes dCas9 with c-terminal fusion of human p300 PHD-HAT domain (aa 1243-1664) (EP300 Human)
    Use
    Lentiviral
    Tags
    Flag Tag
    Expression
    Mammalian
    Mutation
    D10A; H840A in S.pyogenes Cas9
    Promoter
    EF-1alpha
    Available since
    Feb. 17, 2022
    Availability
    Academic Institutions and Nonprofits only
    179545-plasmid-map-sequence-id-354761

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  6. pET-FLAG-eSpCas9-plus

    Plasmid
    #126774
    Purpose
    Expression of increased fidelity eSpCas9-plus in bacterial cells. Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with close to the same fidelity as eSpCas9.
    Depositor
    Ervin Welker
    Publication
    Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:
    Insert
    eSpCas9-plus
    Use
    CRISPR
    Tags
    3xFLAG, 6xHis, MBP, and NLS
    Expression
    Bacterial
    Mutation
    K848A, R1060A; amino acids 1005-1013 replaced wit…
    Promoter
    T7
    Available since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    126774-plasmid-map-sequence-id-410603

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  7. ACBE-Q

    Plasmid
    #204608
    Purpose
    Mammalian expression of high-accuracy adenine-to-cytosine base editor ACBE-Q
    Depositor
    Dali Li
    Publication
    Chen et al Nat Biotechnol. 2023 Jun 15. doi: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    bpNLS-N terminal of nSpCas9-linker-mAAG (R165E/Y179F)-TadA-8e (N108Q)-linker-C terminal of nSpCas9-bpNLS-P2A-GFP
    Use
    CRISPR
    Tags
    6*His
    Expression
    Mammalian
    Mutation
    TadA-8e (N108Q); mAAG (R165E/Y179F); S. pyogenes …
    Available since
    Aug. 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    204608-plasmid-map-sequence-id-404717

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  13. pMVP and pMAGIC Cloning Systems

    Plasmid kit
    #1000000155
    Purpose
    For rapid modular cloning of adenoviral, lentiviral, PiggyBac and Sleeping Beauty transposon vectors for transgene or RNAi delivery (pMVP) and dCas9-based epigenetic engineering (pMAGIC).
    Depositor
    Christopher Newgard
    Application
    Cloning and Synthetic Biology, Genome Editing
    Vector type
    Adenoviral, Lentiviral, Mammalian Expression
    Editing type
    CRISPR
    Cloning type
    MultiSite Gateway
    Available since
    Sept. 6, 2019
    Availability
    Academic Institutions and Nonprofits only

  16. pUF1-dCas9-GCN5

    Plasmid
    #122509
    Purpose
    Combining with the specific sgRNA sequence to activate of the Plasmodium falciparum endogenous gene transcription, through the increasing of H3K9 acetylation.
    Depositor
    Lubin Jiang
    Publication
    Xiao et al Proc Natl Acad Sci U S A. 2019 Jan 2;116
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dCas9-GCN5
    Use
    CRISPR
    Tags
    3*FLAG
    Mutation
    dCas9(D10A,H840A)
    Promoter
    PfHsp86
    Available since
    June 3, 2019
    Availability
    Academic Institutions and Nonprofits only
    122509-plasmid-map-sequence-id-245888

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  18. SPACE (pRZ1813)

    Plasmid
    #140242
    Purpose
    CMV promoter expression plasmid for TadA*(V82G)-nCas9-pmCDA1(R187W)-UGI-UGI-P2A-EGFP
    Depositor
    Keith Joung
    Publication
    Grunewald et al Nat Biotechnol. 2020 Jun 1. pii: 10.1038
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SPACE
    Expression
    Mammalian
    Mutation
    V82G in TadA*, D10A in Cas9, R187W in pmCDA1
    Promoter
    CMV
    Available since
    June 3, 2020
    Availability
    Academic Institutions and Nonprofits only
    140242-plasmid-map-sequence-id-275730

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Showing: 4141 - 4160 of 14352 results