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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 14,359 results for: cas9

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Showing: 4141 - 4160 of 14359 results

  1. U6-sgRNA(MS2)_EF1a-MS2-P65-HSF1

    Plasmid
    #92120
    Purpose
    Expression plasmid for both MS2-P65-HSF1 activator helper complex and sgRNA with two MS2 loops at tetraloop and stemloop 2 contains BsaI sites for insertion of spacer sequences.
    Depositor
    Ervin Welker
    Publication
    Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    MS2-P65-HSF1 (HSF1 Human, Synthetic)
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    EF1a
    Available since
    Oct. 31, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    92120-plasmid-map-sequence-id-197614

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  4. pIVTR(T7A)-Cas9n-RT-T2A-PuroR

    Plasmid
    #172296
    Purpose
    In vitro transcription of Cas9n-RT-T2A-PuroR synthetic construct for PRIME editing
    Depositor
    Patrik Verstreken
    Insert
    Cas9n-RT-T2A-PuroR
    Use
    CRISPR
    Mutation
    H840A (Cas9)
    Promoter
    T7 promoter A-initiating
    Available since
    Sept. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    172296-plasmid-map-sequence-id-340322

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  6. pET-FLAG-SpCas9-HF1-plus

    Plasmid
    #126775
    Purpose
    Expression of increased fidelity SpCas9-HF1-plus in bacterial cells. Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with close to the same fidelity as SpCas9-HF1.
    Depositor
    Ervin Welker
    Publication
    Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:
    Insert
    SpCas9-HF1-plus
    Use
    CRISPR
    Tags
    3xFLAG, 6xHis, MBP, and NLS
    Expression
    Bacterial
    Mutation
    N497A; Q695A; Q926A; amino acids 1005-1013 replac…
    Promoter
    T7
    Available since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    126775-plasmid-map-sequence-id-437774

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  10. pxCas9CR4

    Plasmid
    #111656
    Purpose
    PCas9CR4 with the xCas9 mutations allowing NG PAM sites
    Depositor
    Christopher Reisch
    Insert
    TetR and xCas9
    Use
    Synthetic Biology
    Tags
    ssrA
    Expression
    Bacterial
    Mutation
    xCas9 mutations
    Available since
    July 30, 2018
    Availability
    Academic Institutions and Nonprofits only
    111656-plasmid-map-sequence-id-217189

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  11. pb-CAGGS-dCas9-VP64-blasto

    Plasmid
    #218556
    Purpose
    Encodes CAG promoter-driven dCas9-VP64 with a bipartite SV40 NLS between dCas9 and VP64, along with blasticidin resistance
    Depositor
    Alejandro Chavez
    Publication
    Giddins et al Nat Commun. 2025 Nov 20;16(1):11114. doi
    Insert
    dCas9
    Use
    CRISPR; Piggybac transposition
    Tags
    2xSV40NLS-VP64
    Expression
    Mammalian
    Mutation
    Cas9 mutations to make dCas9=D10A+D839A+H840A+N86…
    Available since
    Aug. 13, 2025
    Availability
    Academic Institutions and Nonprofits only
    218556-plasmid-map-sequence-id-431934

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  12. pME-dCas9-BFP-KRAB

    Plasmid
    #221502
    Purpose
    CRISPRi machinery fish codon optimized
    Depositor
    Alessandro Ori
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dCas9-tagBFP-KRAB
    Use
    Fish expression
    Mutation
    D10A and H839A point mutations
    Available since
    July 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    221502-plasmid-map-sequence-id-439413

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  15. dCas9-p300 PHD-HAT

    Plasmid
    #179545
    Purpose
    encodes S. pyogenes dCas9 with c-terminal fusion of human p300 PHD-HAT domain (aa 1243-1664) driven by EF-1alpha promoter
    Depositor
    Isaac Hilton
    Publication
    Wang et al Nucleic Acids Res. 2022 Aug 12;50(14):78
    Insert
    S. pyogenes dCas9 with c-terminal fusion of human p300 PHD-HAT domain (aa 1243-1664) (EP300 Human)
    Use
    Lentiviral
    Tags
    Flag Tag
    Expression
    Mammalian
    Mutation
    D10A; H840A in S.pyogenes Cas9
    Promoter
    EF-1alpha
    Available since
    Feb. 17, 2022
    Availability
    Academic Institutions and Nonprofits only
    179545-plasmid-map-sequence-id-354761

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  16. pET-FLAG-eSpCas9-plus

    Plasmid
    #126774
    Purpose
    Expression of increased fidelity eSpCas9-plus in bacterial cells. Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with close to the same fidelity as eSpCas9.
    Depositor
    Ervin Welker
    Publication
    Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:
    Insert
    eSpCas9-plus
    Use
    CRISPR
    Tags
    3xFLAG, 6xHis, MBP, and NLS
    Expression
    Bacterial
    Mutation
    K848A, R1060A; amino acids 1005-1013 replaced wit…
    Promoter
    T7
    Available since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    126774-plasmid-map-sequence-id-410603

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  17. ACBE-Q

    Plasmid
    #204608
    Purpose
    Mammalian expression of high-accuracy adenine-to-cytosine base editor ACBE-Q
    Depositor
    Dali Li
    Publication
    Chen et al Nat Biotechnol. 2023 Jun 15. doi: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    bpNLS-N terminal of nSpCas9-linker-mAAG (R165E/Y179F)-TadA-8e (N108Q)-linker-C terminal of nSpCas9-bpNLS-P2A-GFP
    Use
    CRISPR
    Tags
    6*His
    Expression
    Mammalian
    Mutation
    TadA-8e (N108Q); mAAG (R165E/Y179F); S. pyogenes …
    Available since
    Aug. 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    204608-plasmid-map-sequence-id-404717

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Showing: 4141 - 4160 of 14359 results