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pnEA-NpM-HsNot6-R65E_AF

Plasmid

#148663

Purpose

Bacterial Expression of HsNot6-R65E

Depositor

Elisa Izaurralde

Article

Chen et al Nucleic Acids Res. 2021 Jun 21;49(11):64

Insert

HsNot6-R65E (CNOT6 Human)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pnEA-NpM-HsNot6-R87T_AF

Plasmid

#148665

Purpose

Bacterial Expression of HsNot6-R87T

Depositor

Elisa Izaurralde

Article

Chen et al Nucleic Acids Res. 2021 Jun 21;49(11):64

Insert

HsNot6-R87T (CNOT6 Human)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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K202R-short-UBASH3A-V5-6xHis

Plasmid

#195406

Purpose

Mammalian expression of human K202R short-form UBASH3A with V5-6xHis tag

Depositor

Patrick Concannon

Article

Ge et al J Immunol. 2019 Dec 1;203(11):2827-2836.

Insert

UBASH3A (UBASH3A Human)

Tags

V5-6xHis

Expression

Mammalian

Mutation

K202R

Available Since

March 2, 2023

Availability

Academic Institutions and Nonprofits only

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W279A-short-UBASH3A-V5-6xHis

Plasmid

#195409

Purpose

Mammalian expression of human W279A short-form UBASH3A with V5-6xHis tag

Depositor

Patrick Concannon

Article

Ge et al J Immunol. 2019 Dec 1;203(11):2827-2836.

Insert

UBASH3A (UBASH3A Human)

Tags

V5-6xHis

Expression

Mammalian

Mutation

W279A

Available Since

March 2, 2023

Availability

Academic Institutions and Nonprofits only

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Rtn2b-R60f-BFP

Plasmid

#186608

Purpose

Encodes human Reticulon 2 disease mutant R60f fused to BFP

Depositor

Gia Voeltz

Article

Zamponi et al Dev Cell. 2022 Jun 6;57(11):1369-1382.e6

Insert

Human Reticulon 2 mutant R60f (RTN2 Human)

Tags

BFP

Expression

Mammalian

Mutation

c.178insC (R60f)

Promoter

CMV

Available Since

Feb. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pET23PI-Sce ESARC D218A

Plasmid

#195608

Purpose

Expresses PI-SceI (VDE) homing endonuclease/intein from the T7 promoter that contains the D218A active site mutation

Depositor

Frederick Gimble

Article

Gimble et al J Biol Chem. 1995 Mar 17;270(11):5849-56

Insert

PI-SceI

Expression

Bacterial

Mutation

T928C, T929C, A942C

Promoter

T7

Available Since

Feb. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pT7PI-Sce ESARC D326A

Plasmid

#195609

Purpose

Expresses PI-SceI (VDE) homing endonuclease/intein from the T7 promoter that contains the D326A active site mutation

Depositor

Frederick Gimble

Article

Gimble et al J Biol Chem. 1995 Mar 17;270(11):5849-56

Insert

PI-SceI

Expression

Bacterial

Mutation

T3593C, A3601C, T3602G

Promoter

T7

Available Since

Feb. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pET23PI-Sce ESARC

Plasmid

#195607

Purpose

Expresses PI-SceI (VDE) homing endonuclease/intein from the T7 promoter

Depositor

Frederick Gimble

Article

Gimble et al J Biol Chem. 1995 Mar 17;270(11):5849-56

Insert

PI-SceI

Expression

Bacterial

Mutation

C552T,C554A, A557T,G584T, C588A, G878T, C881T, C9…

Promoter

T7

Available Since

Jan. 31, 2023

Availability

Academic Institutions and Nonprofits only

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pTL359

Plasmid

#168420

Purpose

pGBKT7 yeast two-hybrid plasmid

Depositor

Sophien Kamoun

Article

Bentham et al PLoS Pathog. 2021 Nov 10;17(11):e1009957

Insert

OsaHIP41

Expression

Yeast

Available Since

Nov. 30, 2022

Availability

Academic Institutions and Nonprofits only

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pLZRS-panTRK-CR1-EGFP

Plasmid

#187194

Purpose

To express a hybrid Trk receptor tagged with EGFP. This Hybrid TRK receptor was designed to bind NGF, BDNF and NT-3

Depositor

Pantelis Tsoulfas

Article

O'Connell et al J Biol Chem. 2000 Mar 17;275(11):7870-7.

Insert

CR1 pan-TRK receptor

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Promoter

mo-MLV or MMLV

Available Since

Nov. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-CrPV-Rluc-6xbsMS2-PolyA-Malat_AC

Plasmid

#148446

Purpose

Mammalian Expression of 6xbsMS2-PolyA-Malat

Depositor

Elisa Izaurralde

Article

Kuzuoglu-Ozturk et al EMBO J. 2016 Jun 1;35(11):1186-203. doi:

Insert

6xbsMS2-PolyA-Malat

Expression

Mammalian

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HCV-RLuc-6xbsMS2-polyA-Malat_AC

Plasmid

#148448

Purpose

Mammalian Expression of 6xbsMS2-polyA-Malat

Depositor

Elisa Izaurralde

Article

Kuzuoglu-Ozturk et al EMBO J. 2016 Jun 1;35(11):1186-203. doi:

Insert

6xbsMS2-polyA-Malat

Expression

Mammalian

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pMLS640

Plasmid

#188892

Purpose

ttTi5605 MosSci targeting vector with Pmex-5::Cas9 and floxed Cbr-unc-119 marker

Depositor

Erik Jorgensen

Article

Schwartz et al PLoS Genet. 2021 Nov 8;17(11):e1009755.

Insert

Pmex-5::Cas9, Cbr-unc-119

Expression

Worm

Mutation

C. elegans codom optimization and intron addition

Available Since

Sept. 6, 2022

Availability

Academic Institutions and Nonprofits only

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pMLS538

Plasmid

#188881

Purpose

Gateway Entry Vector [1-2]: 2xNLS::Cas9 (with 4 introns from smu-2)

Depositor

Erik Jorgensen

Article

Schwartz et al PLoS Genet. 2021 Nov 8;17(11):e1009755.

Insert

Cas9

Use

Gateway entry vector

Mutation

C. elegans codom optimization and intron addition

Available Since

Aug. 30, 2022

Availability

Academic Institutions and Nonprofits only

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pPBO.ACT002

Plasmid

#182712

Purpose

aTc inducible dCasRx-IF1

Depositor

Joseph Schoeniger

Article

Otoupal et al Nucleic Acids Res. 2022 Aug 11. pii: 666

Insert

dCasRx

Use

CRISPR

Tags

3x(GGGS)-Escherichia Coli Initiation Factor 1

Expression

Bacterial

Mutation

Catalytically deactivated R295A, H300A, R849A, H8…

Promoter

pTet

Available Since

Aug. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pTL335

Plasmid

#168397

Purpose

pGBKT7 yeast two-hybrid plasmid

Depositor

Sophien Kamoun

Article

Bentham et al PLoS Pathog. 2021 Nov 10;17(11):e1009957

Insert

OsaHIP24

Expression

Yeast

Available Since

Aug. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pTL336

Plasmid

#168396

Purpose

pGBKT7 yeast two-hybrid plasmid

Depositor

Sophien Kamoun

Article

Bentham et al PLoS Pathog. 2021 Nov 10;17(11):e1009957

Insert

OsaHIP34

Expression

Yeast

Available Since

Aug. 8, 2022

Availability

Academic Institutions and Nonprofits only

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gSCPL35-mRFP

Plasmid

#181960

Purpose

SCPL35 protein tagged with mRFP, under control of native promoter

Depositor

George Haughn

Article

Lee et al Plant Direct. 2021 Nov 28;5(11):e360. do

Insert

SCPL35

Tags

mRFP

Expression

Plant

Promoter

native SCPL35

Available Since

March 14, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmLSm2_A

Plasmid

#145887

Purpose

Insect Expression of DmLSm2

Depositor

Elisa Izaurralde

Article

Tritschler et al Mol Cell Biol. 2007 Dec . 27(24):8600-11

Insert

DmLSm2 (CG10418 Fly)

Expression

Insect

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmLSm5_A

Plasmid

#145890

Purpose

Insect Expression of DmLsm5

Depositor

Elisa Izaurralde

Article

Tritschler et al Mol Cell Biol. 2007 Dec . 27(24):8600-11

Insert

DmLsm5 (CG6610 Fly)

Expression

Insect

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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