1. p426 103Q GPD

   Plasmid

   #1184

   Depositor

   Susan Lindquist

   Article

   Krobitsch et al Proc Natl Acad Sci U S A 2000 Feb 15;97(

   Insert

   htt 103Q (HTT Human)
   

   Use

   Tags

   GFP

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Availability

   Academic Institutions and Nonprofits only

These results may also be relevant.

1. pX459-HypaCas9-mR2-RHOA_sgRNA

   Plasmid

   #183877

   Purpose

   pX459V2.0-HypaCas9 plasmid with mRuby2 reporter and RHOA sgRNA for N-terminal tagging of RhoA in human cells.

   Depositor

   Alisa Piekny

   Article

   Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

   Insert

   RHOA sgRNA spacer (RHOA Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   May 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pLEX304-ARF5-mNeonGreen

   Plasmid

   #162025

   Purpose

   Expression of tagged ARF WT

   Depositor

   David Bryant

   Article

   Nacke et al Nat Commun. 2021 Mar 12;12(1):1623. doi:

   Insert

   ARF5 (ARF5 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. psiCHECK2-E2F3 3'UTR

   Plasmid

   #29470

   Depositor

   Judy Lieberman

   Article

   Lal et al Mol Cell. 2009 Sep 11. 35(5):610-25.

   Insert

   E2F3 3'UTR (E2F3 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 27, 2011

   Availability

   Academic Institutions and Nonprofits only

4. pEGFP-N3/hArf5(WT)-EGFP

   Plasmid

   #79534

   Purpose

   Expresses C-terminally EGFP-tagged Arf5(WT) in mammalian cells

   Depositor

   Kazuhisa Nakayama

   Article

   Makyio et al EMBO J. 2012 May 30;31(11):2590-603. doi

   Insert

   Arf5 (ARF5 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pDEST47-ARF4-GFP

   Plasmid

   #67392

   Purpose

   Mammalian expression of hArf4 with C-term GFP tag

   Depositor

   Richard Kahn

   Article

   Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

   Insert

   ARF4 (ARF4 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   V53A, L123P and M134I mutations were unintended P…

   Promoter

   CMV

   Available since

   Oct. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

6. pGEX Arg-SH2

   Plasmid

   #46405

   Depositor

   Bruce Mayer

   Article

   Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

   Insert

   Arg/Abl2 (ABL2 Human)
   

   Use

   Tags

   GST and PreScission

   Expression

   Bacterial

   Mutation

   contains amino acids 151-257

   Promoter

   Tac

   Available since

   July 12, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pIS1-mutant RhoA 3'UTR

   Plasmid

   #26090

   Depositor

   Bob Weinberg

   Article

   Valastyan et al Cell. 2009 Jun 12. 137(6):1032-46.

   Insert

   mutant RhoA 3'UTR (RHOA Human)
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Both miR-31 binding sites in 3’ UTR mutagenized. …

   Promoter

   Available since

   Oct. 25, 2010

   Availability

   Academic Institutions and Nonprofits only

8. pmNeon Green-N1-ARF1-R

   Plasmid

   #202430

   Purpose

   Mammalian expression vector containing mNeonGreen-tagged ARF1

   Depositor

   David Bryant

   Article

   Sandilands et al J Cell Biol. 2023 Apr 3;222(4):e20220611

   Insert

   ARF1 (ARF1 Human)
   

   Use

   Lentiviral

   Tags

   mNeonGreen

   Expression

   Mutation

   Promoter

   Available since

   April 28, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pmNeon Green-N1-ARF3-R

   Plasmid

   #202431

   Purpose

   Mammalian expression vector containing pmNeonGreen-tagged ARF3

   Depositor

   David Bryant

   Article

   Sandilands et al J Cell Biol. 2023 Apr 3;222(4):e20220611

   Insert

   ARF3 (ARF3 Human)
   

   Use

   Lentiviral

   Tags

   mNeonGreen

   Expression

   Mutation

   Promoter

   Available since

   March 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. mRFP-PA-GFP-RhoAQ63L

    Plasmid

    #187282

    Purpose

    Express pmRFP-PAGFP-RhoA Q63L

    Depositor

    Alpha Yap

    Article

    Budnar et al Dev Cell. 2019 Jun 17;49(6):894-906.e12.

    Insert

    RhoA (RHOA Human)
    

    Use

    Tags

    mRFP-PAGFP

    Expression

    Mammalian

    Mutation

    RhoA Q63L

    Promoter

    CMV, T7

    Available since

    Oct. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3.1(+)-GFP11-RhoA

    Plasmid

    #181860

    Purpose

    Part of FluoSTEP-RhoA biosensor; co-express with cpPKN-mRuby2-GFP1-10 (Addgene #181859).

    Depositor

    Jin Zhang

    Article

    Tenner et al Sci Adv. 2021 May 21;7(21). pii: 7/21/ea

    Insert

    GFP11-RhoA (RHOA Human)
    

    Use

    Tags

    GFP11

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pDONR221-ARF4-no stop

    Plasmid

    #67311

    Purpose

    hArf4 without stop codon in Gateway Entry vector

    Depositor

    Richard Kahn

    Article

    Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

    Insert

    ARF4 (ARF4 Human)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Bacterial

    Mutation

    V53A, L123P and M134I mutations were unintended P…

    Promoter

    Available since

    Oct. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

13. TFORF1704

    Plasmid

    #143308

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    E2F3 (E2F3 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    July 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pcDNA3/hArf6(WT)-mCherry

    Plasmid

    #79422

    Purpose

    Expresses C-terminally mCherry-tagged Arf6(WT) in mammalian cells

    Depositor

    Kazuhisa Nakayama

    Article

    Makyio et al EMBO J. 2012 May 30;31(11):2590-603. doi

    Insert

    Arf6 (ARF6 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pcDNA3/hArf6(Q67L)-HA

    Plasmid

    #79425

    Purpose

    Expresses C-terminally HA-tagged Arf6(Q67L) in mammalian cells

    Depositor

    Kazuhisa Nakayama

    Article

    Makyio et al EMBO J. 2012 May 30;31(11):2590-603. doi

    Insert

    Arf6 (ARF6 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    changed Glutamine 67 to Leucine

    Promoter

    Available since

    April 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pcDNA3/hArf6(T27N)-HA

    Plasmid

    #79426

    Purpose

    Expresses C-terminally HA-tagged Arf6(T27N) in mammalian cells

    Depositor

    Kazuhisa Nakayama

    Article

    Makyio et al EMBO J. 2012 May 30;31(11):2590-603. doi

    Insert

    Arf6 (ARF6 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    changed Threonine 27 to Asparagine

    Promoter

    Available since

    April 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pLEX301-ARF6-TagRFP

    Plasmid

    #162029

    Purpose

    Expression of tagged ARF WT

    Depositor

    David Bryant

    Article

    Nacke et al Nat Commun. 2021 Mar 12;12(1):1623. doi:

    Insert

    ARF6 (ARF6 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. GPS-MYC

    Plasmid

    #160130

    Purpose

    A reporter that monitors MYC protein expression.

    Depositor

    Channing Der

    Article

    Blake et al Sci Signal. 2019 Jul 16;12(590). pii: 12

    Insert

    MYC (MYC Human)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA3/hArf1(WT)-mCherry

    Plasmid

    #79419

    Purpose

    Expresses C-terminally mCherry-tagged Arf1(WT) in mammalian cells

    Depositor

    Kazuhisa Nakayama

    Article

    Makyio et al EMBO J. 2012 May 30;31(11):2590-603. doi

    Insert

    Arf1 (ARF1 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pTriEx-RhoA-wt_mScarlet-i_SGFP2

    Plasmid

    #85071

    Purpose

    Green/red FRET biosensor to monitor RhoA nucleotide loading state and activity

    Depositor

    Dorus Gadella

    Article

    Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

    Insert

    RhoA Biosensor wildtype with mScarlet-i and SGFP2 (RHOA Human)
    

    Use

    Tags

    6xHis and mScarlet-i and SGFP2

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    Promoter

    CMV

    Available since

    Dec. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge