We narrowed to 584 results for: Nut

1. HBT-CPK30-Flag

   Plasmid

   #107430

   Purpose

   For Arabidopsis mesophyll protoplast transient expression assay

   Depositor

   Jen Sheen

   Article

   Liu et al Nature. 2017 May 18;545(7654):311-316. d

   Insert

   CPK30 (CPK30 Mustard Weed)
   

   Use

   Tags

   Flag

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET28-p53 (1-73) (K24N)

   Plasmid

   #62079

   Purpose

   Expression of human p53 (1-73) (K24N) in e. coli

   Depositor

   Gary Daughdrill

   Article

   Zhan et al Proteins. 2013 Oct;81(10):1738-47. doi:

   Insert

   p53 (TP53 Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   contains TAD residues 1-73; K24N

   Promoter

   T7

   Available since

   Jan. 29, 2015

   Availability

   Academic Institutions and Nonprofits only

3. pLJC6-2XFLAG-TMEM192

   Plasmid

   #104435

   Purpose

   Expresses 2XFLAG-TMEM192 in mammalian cells

   Depositor

   David Sabatini

   Article

   Wyant et al Cell. 2017 Oct 19;171(3):642-654.e12. do

   Insert

   TMEM-192 (TMEM192 Human)
   

   Use

   Lentiviral

   Tags

   2XFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Ubiquitin

   Available since

   July 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLJM1 TMEM192-Flag-FKBP

   Plasmid

   #112743

   Purpose

   express TMEM192-Flag-FKBP

   Depositor

   Roberto Zoncu

   Article

   Lawrence et al Nat Cell Biol. 2018 Sep;20(9):1052-1063.

   Insert

   TMEM192-Flag-FKBP (TMEM192 Human)
   

   Use

   Lentiviral

   Tags

   Flag, FKBP

   Expression

   Mutation

   Promoter

   Available since

   Oct. 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. K-to-R mutant NPRL2(1-380) in pLJM60

   Plasmid

   #184562

   Purpose

   CMV-driven expression of an untagged mutant NPRL2 (core subunit of GATOR1) — native sequence for stable expression in mammalian cells. All lysine residues were mutated to arginines.

   Depositor

   Kacper Rogala

   Article

   Valenstein et al Nature. 2022 Jul;607(7919):610-616. doi:

   Insert

   NPRL2 (NPRL2 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   All lysines were mutated to arginines.

   Promoter

   CMV

   Available since

   Nov. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLJM1 Flag GFP 4E-BP1 Rheb15

   Plasmid

   #112761

   Purpose

   express GFP-4EBP1-Rheb15

   Depositor

   Roberto Zoncu

   Article

   Lawrence et al Nat Cell Biol. 2018 Sep;20(9):1052-1063.

   Insert

   Flag-GFP-4EBP1-Rheb15 (EIF4EBP1 Human)
   

   Use

   Lentiviral

   Tags

   Flag eGFP and Rheb15

   Expression

   Mutation

   Promoter

   Available since

   Nov. 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pRK5 GST MetaP2

   Plasmid

   #112750

   Purpose

   express HA-GST-FRB-MetaP2

   Depositor

   Roberto Zoncu

   Article

   Lawrence et al Nat Cell Biol. 2018 Sep;20(9):1052-1063.

   Insert

   HA-GST-FRB-MetaP2 (METAP2 Human)
   

   Use

   Tags

   GST

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. KHBD00297

   Plasmid

   #34423

   Depositor

   Bart Deplancke

   Article

   Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

   Insert

   CG10798 (Myc Fly)
   

   Use

   Gateway donor vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 23, 2012

   Availability

   Academic Institutions and Nonprofits only

9. pLJM1 Flag GFP 4E-BP1 Omp25

   Plasmid

   #112760

   Purpose

   express GFP-4EBP1-Omp25

   Depositor

   Roberto Zoncu

   Article

   Lawrence et al Nat Cell Biol. 2018 Sep;20(9):1052-1063.

   Insert

   Flag-GFP-4EBP1-Omp25 (EIF4EBP1 Human)
   

   Use

   Lentiviral

   Tags

   Flag eGFP and Omp25

   Expression

   Mutation

   Promoter

   Available since

   Oct. 16, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLJC2-DHFR-3xFLAG

    Plasmid

    #217428

    Purpose

    Lentiviral overexpression of human DHFR

    Depositor

    Jason Cantor

    Article

    Flickinger et al Sci Adv. 2024 Oct 4;10(40):eadq3591. doi

    Insert

    DHFR (DHFR Human)
    

    Use

    Lentiviral

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Insert sequence is a codon-optimized gBLOCK (IDT)

    Promoter

    CMV

    Available since

    Jan. 31, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLentiCRISPR-v1-sgABCC4_1

    Plasmid

    #217433

    Purpose

    Lentiviral vector expressing Cas9 and an sgRNA targeting human ABCC4

    Depositor

    Jason Cantor

    Article

    Flickinger et al Sci Adv. 2024 Oct 4;10(40):eadq3591. doi

    Insert

    sgRNA 1 targeting ABCC4 (ABCC4 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Jan. 31, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLentiCRISPR-v1-sgABCC4_3

    Plasmid

    #217434

    Purpose

    Lentiviral vector expressing Cas9 and an sgRNA targeting human ABCC4

    Depositor

    Jason Cantor

    Article

    Flickinger et al Sci Adv. 2024 Oct 4;10(40):eadq3591. doi

    Insert

    sgRNA 3 targeting ABCC4 (ABCC4 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Jan. 31, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. LMPd Amt LDHA

    Plasmid

    #209408

    Purpose

    Retroviral vector with Ametrine marker for expressing shRNA with an "UltramiR" microRNA scaffold

    Depositor

    Russell Jones

    Article

    Kaymak et al Cell Metab. 2022 Sep 6;34(9):1298-1311.e

    Insert

    LdhA shRNA (Ldha Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. EcNGsC-F307Y-pKK223

    Plasmid

    #131381

    Purpose

    Low level expression of E.coli G.stearothermophillus MutY chimera protein with amino acid change F307Y in Gs MutY.

    Depositor

    Martin Horvath

    Article

    Russelburg et al ACS Chem Biol. 2019 Dec 27. doi: 10.1021

    Insert

    EcNGsC MutY chimera F307Y
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Fusion of two MutY genes. Residues 1-225 are from…

    Promoter

    tac

    Available since

    Jan. 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. EcNGsC-F307A-pKK223

    Plasmid

    #131382

    Purpose

    Low level expression of E.coli G.stearothermophillus MutY chimera protein with amino acid change F307A in Gs MutY.

    Depositor

    Martin Horvath

    Article

    Russelburg et al ACS Chem Biol. 2019 Dec 27. doi: 10.1021

    Insert

    EcNGsC MutY chimera F307A
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Fusion of two MutY genes. Residues 1-225 are from…

    Promoter

    tac

    Available since

    Jan. 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. EcNGsC-S308V-pKK223

    Plasmid

    #131393

    Purpose

    Low level expression of E.coli G.stearothermophillus MutY chimera protein with amino acid change S308V in Gs MutY.

    Depositor

    Martin Horvath

    Article

    Russelburg et al ACS Chem Biol. 2019 Dec 27. doi: 10.1021

    Insert

    EcNGsC MutY chimera S308V
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Fusion of two MutY genes. Residues 1-225 are fro…

    Promoter

    tac

    Available since

    Jan. 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. EcNGsC-S308A-pKK223

    Plasmid

    #131394

    Purpose

    Low level expression of E.coli G.stearothermophillus MutY chimera protein with amino acid change S308A in Gs MutY.

    Depositor

    Martin Horvath

    Article

    Russelburg et al ACS Chem Biol. 2019 Dec 27. doi: 10.1021

    Insert

    EcNGsC MutY chimera S308A
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Fusion of two MutY genes. Residues 1-225 are fro…

    Promoter

    tac

    Available since

    Jan. 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pMS22H-IRE

    Plasmid

    #133905

    Purpose

    Positive control when used in combination with pAWH-IRP. Expression of MS2BS-IRE site hybrid. Homology regions for recombination with pAWH

    Depositor

    Sebastian Maurer

    Article

    Yang et al Nat Commun. 2018 Sep 14;9(1):3747. doi:

    Insert

    IRE (Ftl1 Rat)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Pol III

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

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19. nLuc-GLP-1R-His-FLAG

    Plasmid

    #124831

    Purpose

    Expresses N-terminally NanoLuc-Tagged GLP-1R in mammalian cells

    Depositor

    Sam Gellman

    Article

    Cary et al Nat Chem Biol. 2022 Mar;18(3):256-263. d

    Insert

    GLP1R (GLP1R Human, Synthetic)
    

    Use

    Luciferase

    Tags

    FLAG, His6, and NanoLuc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCAG-eSpCas9-2A-GFP-sgRNA-DNA-PKcs

    Plasmid

    #220493

    Purpose

    To generate DNA-PKcs KO in human cells. Co-expresses eSpCas9(1.1), GFP and a guide RNA against DNA-PKcs exon 1.

    Depositor

    Sébastien Britton

    Article

    Bossaert et al bioRxiv [Preprint]. 2024 Jan 4:2024.01.0

    Insert

    sgRNA targeting DNA-PKcs (PRKDC) exon 1 (PRKDC Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    July 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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