We narrowed to 3,869 results for: SPL;

1. HJP-179-IVS-Syn21-NrdJ-1C-CreC-WPREw

   Plasmid

   #162472

   Purpose

   This insert, when expressed in the same cell with other split Cre component, forms Cre recombinase through protein trans-splicing using split intein.

   Depositor

   Benjamin White

   Article

   Luan et al Elife. 2020 Apr 14;9. pii: 53041. doi: 1

   Insert

   CreC
   

   Use

   Cre/Lox

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. HJP-176-IVS-Syn21-CreA-gp41-1N-WPREw

   Plasmid

   #162470

   Purpose

   This insert, when expressed in the same cell with other split Cre component, forms Cre recombinase through protein trans-splicing using split intein.

   Depositor

   Benjamin White

   Article

   Luan et al Elife. 2020 Apr 14;9. pii: 53041. doi: 1

   Insert

   CreA
   

   Use

   Cre/Lox

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. HJP-196-IVS-Syn21-NrdJ-1C-CreC-WPREUw

   Plasmid

   #162477

   Purpose

   This insert, when expressed in the same cell with other split Cre component, forms Cre recombinase through protein trans-splicing using split intein.

   Depositor

   Benjamin White

   Article

   Luan et al Elife. 2020 Apr 14;9. pii: 53041. doi: 1

   Insert

   CreC
   

   Use

   Cre/Lox

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. HJP-180-IVS-Syn21-gp41-1C-CreB-NrdJ-1N-WPREw

   Plasmid

   #162471

   Purpose

   This insert, when expressed in the same cell with other split Cre component, forms Cre recombinase through protein trans-splicing using split intein.

   Depositor

   Benjamin White

   Article

   Luan et al Elife. 2020 Apr 14;9. pii: 53041. doi: 1

   Insert

   CreB
   

   Use

   Cre/Lox

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. CMV-LUC2CP/ARE

   Plasmid

   #62857

   Purpose

   Splicing reporter control (no intron) with elements to shorten the half-life of the luciferase protein as well as the luciferase mRNA.

   Depositor

   Gideon Dreyfuss

   Article

   Younis et al Mol Cell Biol. 2010 Apr;30(7):1718-28. d

   Insert

   Luciferase
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   destabilizing sequences (DS) added to the C termi…

   Promoter

   CMV

   Available since

   April 3, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pAT9651-BEAR-GFP

   Plasmid

   #162989

   Purpose

   BEAR target plasmid with split EGFP and disrupted 5' splice site

   Depositor

   Ervin Welker

   Article

   Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

   Insert

   EGFP split with an intron between amino acids 95-96
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. Nrxn3α-AS4-

   Plasmid

   #184332

   Purpose

   Expression of mouse Nrx3-alpha lacking insertion at the alternative splice site 4 together with EGFP protein separated by 2A peptide. Contains insertions at alternatively splice sites 1,2,3 and 6.

   Depositor

   Peter Scheiffele

   Article

   Nguyen et al Elife. 2016 Dec 13;5. doi: 10.7554/eLife

   Insert

   Nrxn3-alpha (Nrxn3 Mouse)
   

   Use

   Tags

   HA-tag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. Nrxn3α-AS4+

   Plasmid

   #184333

   Purpose

   Expression of mouse Nrx3-alpha containing insertion at the alternative splice site 4 together with EGFP protein separated by 2A peptide. Contains insertions at alternatively splice sites 1,2,3 and 6.

   Depositor

   Peter Scheiffele

   Article

   Nguyen et al Elife. 2016 Dec 13;5. doi: 10.7554/eLife

   Insert

   Nrxn3-alpha (Nrxn3 Mouse)
   

   Use

   Tags

   HA-tag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pAT9750-BEAR-mCherry

   Plasmid

   #162990

   Purpose

   BEAR target plasmid with split mCherry and disrupted 5' splice site

   Depositor

   Ervin Welker

   Article

   Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

   Insert

   mCherry split with an intron between amino acids 119-120
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pFlare9A-Plekha5E10 WT

    Plasmid

    #209576

    Purpose

    minigene construct for Plekha5 E10 alternative splicing

    Depositor

    Sika Zheng

    Article

    Zhang et al Neuron. 2019 Feb 20;101(4):690-706.e10.

    Insert

    Plekha5 (Plekha5 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. CMV-LUC2CP/intron/ARE

    Plasmid

    #62858

    Purpose

    splicing reporter (with intron) with elements to shorten the half-life of the luciferase protein as well as the luciferase mRNA.

    Depositor

    Gideon Dreyfuss

    Article

    Younis et al Mol Cell Biol. 2010 Apr;30(7):1718-28. d

    Insert

    Luciferase
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    insertion of chimeric intron at nueotide poclsiti…

    Promoter

    CMV

    Available since

    April 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pETcon-pGAL1-Aga2-α-Synuclein-c_myc

    Plasmid

    #225222

    Purpose

    Yeast optimized human alpha synuclein fused with aga2 protein gene under gal1 promoter for the expression of alpha synuclein in yeast surface display.

    Depositor

    Urartu Seker

    Article

    Ozcelik et al ACS Chem Neurosci. 2023 Oct 4;14(19):360

    Inserts

    α-Synuclein
    Aga2
    

    Use

    Tags

    GS linker, HA Tag, and c-myc Tag

    Expression

    Yeast

    Mutation

    Promoter

    GAL1

    Available since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pFlare9A-Clip2E9 WT

    Plasmid

    #209574

    Purpose

    minigene construct for Clip2 E9 alternative splicing

    Depositor

    Sika Zheng

    Article

    Zhang et al Neuron. 2019 Feb 20;101(4):690-706.e10.

    Insert

    Clip2 (Clip2 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pTET GFP10-FRB/FKBP-GFP11

    Plasmid

    #182497

    Purpose

    Tripartite split-GFP. E. coli vector. Inducible coexpression of GFP10-FRB and FKBP-GFP11 coiled coils

    Depositor

    Stéphanie Cabantous

    Article

    Pedelacq et al Methods Mol Biol. 2019;2025:423-437. doi

    Inserts

    FRB
    FKBP (Fkbp1a Mouse, Synthetic)
    

    Use

    Tags

    split GFP10 tripartite and split GFP11 tripartite

    Expression

    Bacterial

    Mutation

    Promoter

    TET

    Available since

    April 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pETcon-pGAL1-Aga2-Amyloid β42-c_myc

    Plasmid

    #225223

    Purpose

    Yeast optimized human Amyloid β42 fused with aga2 protein gene under gal1 promoter for the expression of Amyloid β42 in yeast surface display.

    Depositor

    Urartu Seker

    Article

    Ozcelik et al ACS Chem Neurosci. 2023 Oct 4;14(19):360

    Inserts

    Amyloid β42
    Aga2 (AGA2 Budding Yeast)
    

    Use

    Tags

    HA Tag and c-myc Tag

    Expression

    Yeast

    Mutation

    Promoter

    GAL1

    Available since

    Feb. 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pFlare9A-Clip2E9 Mutant

    Plasmid

    #209575

    Purpose

    minigene construct for Clip2 E9 alternative splicing, potential PTBP2 binding sites are deleted

    Depositor

    Sika Zheng

    Article

    Zhang et al Neuron. 2019 Feb 20;101(4):690-706.e10.

    Insert

    Clip2 (Clip2 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    ACGCGTTTCTGAATTCTTCTAACTGCCCTCAAATGCACGGTGGCATGTG…

    Promoter

    CMV

    Available since

    March 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCR2.1_HBB_A

    Plasmid

    #87848

    Purpose

    pCR2.1-based plasmid holding a 328-bp fragment containing the exon-1-exon-2 splice border of aberrant HBB[IVSI-110(G>A)] human β-globin mRNA

    Depositor

    Marina Kleanthous

    Article

    Patsali et al Int J Mol Sci. 2020 Sep 11;21(18). pii:

    Insert

    Homo sapiens hemoglobin subunit beta (HBB), Aberrant cDNA fragment (Exon1/19nt mispliced Intron1 + Exon2) (HBB Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    HBB(IVSI-110) β-thalassaemia misplicing mutation …

    Promoter

    Available since

    Oct. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pJF3H

    Plasmid

    #63897

    Purpose

    For phage display of cDNA libraries

    Depositor

    Carlos Barbas

    Article

    Barbas et al Cold Spring Harbor, NY: Cold Spring Harb

    Insert

    cDNA cloning region
    

    Use

    Phage display

    Tags

    Fos

    Expression

    Bacterial

    Mutation

    Promoter

    lacZ

    Available since

    July 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pB33eCPX

    Plasmid

    #23336

    Depositor

    Patrick Daugherty

    Article

    Rice et al Protein Eng Des Sel. 2008 Jul . 21(7):43

    Insert

    Enhanced Circularly Permuted Outer Membrane Protein X
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 9, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. p2-39

    Plasmid

    #118086

    Purpose

    ZB-linker-split C-terminal T7 RNAP expression plasmid

    Depositor

    Bryan Dickinson

    Article

    Pu et al Nat Chem Biol. 2017 Apr;13(4):432-438. d

    Insert

    ZB-linker-split C-terminal T7 RNAP expression plasmid
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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