Addgene

We narrowed to 1,480 results for: pBI

Showing: 401 - 420 of 1480 results

Results per page:

Sort results by:

pbIG-Xph18-Stg

Plasmid

#133037

Purpose

Expresses Xph18-Stg in bacteria

Depositor

Matthieu Sainlos

Article

Rimbault et al Nat Commun. 2019 Oct 4;10(1):4521. doi:

Insert

Xph18-Stg

Use

Tags

AviTag, His10, and TEV protease cleavage site

Expression

Bacterial

Mutation

S63K

Promoter

T7

Available since

Nov. 25, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pbIG-Xph15-ETWV

Plasmid

#133038

Purpose

Expresses Xph15-ETWV in bacteria

Depositor

Matthieu Sainlos

Article

Rimbault et al Nat Commun. 2019 Oct 4;10(1):4521. doi:

Insert

Xph15-ETWV

Use

Tags

AviTag, His10, and TEV protease cleavage site

Expression

Bacterial

Mutation

S63K

Promoter

T7

Available since

Nov. 25, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pbIG-Xph0-ETWV

Plasmid

#133039

Purpose

Expresses Xph0-ETWV in bacteria

Depositor

Matthieu Sainlos

Article

Rimbault et al Nat Commun. 2019 Oct 4;10(1):4521. doi:

Insert

Xph0-ETWV

Use

Tags

AviTag, His10, and TEV protease cleavage site

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 25, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pBITE-EGFRvIII-F265-OKT3

Plasmid

#190679

Purpose

Encodes an EGFRvIII-CD3 BITE protein (F265 clone)

Depositor

Scott McComb

Article

Shepherd et al PLoS One. 2023 Jun 22;18(6):e0273884. do

Insert

Anti-EGFRvIII-scFv-F265-modularized-OKT3-HisTag

Use

Lentiviral

Tags

mNeonGreen

Expression

Mammalian

Mutation

Promoter

EFS

Available since

Feb. 21, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
Yellow Cameleon-Nano15/pBig

Plasmid

#51962

Purpose

Expression of Yellow Cameleon-Nano15, an ultrasensitive Ca2+ indicator with Kd = 15 nM, in Dictyostelium

Depositor

Takeharu Nagai

Article

Horikawa et al Nat Methods. 2010 Sep;7(9):729-32. doi:

Insert

Yellow Cameleon-Nano15

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 15, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pBITE-EGFRvIII-F269-OKT3

Plasmid

#190681

Purpose

Encodes an EGFRvIII-CD3 BITE protein (F269 clone)

Depositor

Scott McComb

Article

Shepherd et al PLoS One. 2023 Jun 22;18(6):e0273884. do

Insert

Anti-EGFRvIII-scFv-F269-modularized-OKT3-HisTag

Use

Lentiviral

Tags

mNeonGreen

Expression

Mammalian

Mutation

Promoter

EFS

Available since

Feb. 21, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pBI-p53 R175H/EGFP

Plasmid

#16544

Depositor

Bert Vogelstein

Article

Yu et al Proc Natl Acad Sci U S A. 1999 Dec 7. 96

Insert

p53 (TP53 Human)

Use

Tags

Expression

Mammalian

Mutation

R175H

Promoter

Available since

June 20, 2008

Availability

Academic Institutions and Nonprofits only

Enlarge
PyronicSF-mRuby2/pBI-CMV1

Plasmid

#124830

Purpose

Highly resposive GFP-based pyruvate nanosensor.

Depositor

Luis Felipe Barros

Article

Arce-Molina et al eLife. 2020 Mar 6;9. pii: 53917. doi: 10

Inserts

PyronicSF
mRuby2

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 13, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pBITE-EGFRvIII-F263-OKT3

Plasmid

#190680

Purpose

Encodes an EGFRvIII-CD3 BITE protein (F263 clone)

Depositor

Scott McComb

Article

Shepherd et al PLoS One. 2023 Jun 22;18(6):e0273884. do

Insert

Anti-EGFRvIII-scFv-F263-modularized-OKT3-HisTag

Use

Lentiviral

Tags

mNeonGreen

Expression

Mammalian

Mutation

Promoter

EFS

Available since

Feb. 21, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pBITE-EGFRvIII-F271-OKT3

Plasmid

#190682

Purpose

Encodes an EGFRvIII-CD3 BITE protein (F271 clone)

Depositor

Scott McComb

Article

Shepherd et al PLoS One. 2023 Jun 22;18(6):e0273884. do

Insert

Anti-EGFRvIII-scFv-F271-modularized-OKT3-HisTag

Use

Lentiviral

Tags

mNeonGreen

Expression

Mammalian

Mutation

Promoter

EFS

Available since

Feb. 21, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pBiFC-HA-hCaspase 2 prodomain-VC155

Plasmid

#49261

Purpose

Expresses split fluorescence protein to measure caspase-2 dimerization in mammalian cells.

Depositor

Douglas Green

Article

Bouchier-Hayes et al Mol Cell. 2009 Sep 24;35(6):830-40. doi:

Insert

caspase-2 prodomain (CASP2 Human)

Use

Tags

HA and split Venus C

Expression

Mammalian

Mutation

delete aa 148-435

Promoter

CMV

Available since

Dec. 9, 2013

Availability

Academic Institutions and Nonprofits only
pBI-GFP-Bub1-S492Y

Plasmid

#16625

Depositor

Bert Vogelstein

Article

Cahill et al Nature. 1998 Mar 19. 392(6673):300-3.

Insert

BUB1 S492Y (BUB1 Human)

Use

Tags

Expression

Mammalian

Mutation

S492Y

Promoter

Available since

March 12, 2008

Availability

Academic Institutions and Nonprofits only
pBiFC-HA-hCaspase 2 CARD-VC155

Plasmid

#49259

Purpose

Expresses split fluorescence protein to measure caspase-2 dimerization in mammalian cells.

Depositor

Douglas Green

Article

Bouchier-Hayes et al Mol Cell. 2009 Sep 24;35(6):830-40. doi:

Insert

Caspase-2 CARD (CASP2 Human)

Use

Tags

HA and split Venus C

Expression

Mammalian

Mutation

deleted aa 123-435

Promoter

CMV

Available since

Dec. 9, 2013

Availability

Academic Institutions and Nonprofits only
pBiFC-HA-hCaspase 2 FL (C303S)-VC155

Plasmid

#49257

Purpose

Expresses split fluorescence protein to measure caspase-2 dimerization in mammalian cells.

Depositor

Douglas Green

Article

Bouchier-Hayes et al Mol Cell. 2009 Sep 24;35(6):830-40. doi:

Insert

caspase-2 (CASP2 Human)

Use

Tags

HA and split Venus C

Expression

Mammalian

Mutation

C303S

Promoter

CMV

Available since

March 14, 2014

Availability

Academic Institutions and Nonprofits only
pCDNA3/FKBP12-pBirA(284-C)-HA

Plasmid

#154452

Purpose

expresses FKBP12-pBirA(284-C)-HA/biochemical assay

Depositor

Hyun-Woo Rhee

Article

Kwak et al Proc Natl Acad Sci U S A. 2020 May 15. p

Insert

FKBP12-pBirA(284-C)-HA (FKBP12 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

T7 promoter

Available since

Aug. 24, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pBIG1e-CDK12-KiD/Cyclin K

Plasmid

#231743

Purpose

Protein expression in insect cells

Depositor

Jesper Svejstrup

Article

Martinez et al Molecular Cell. 01 Mar 2025. doi: 10.101

Inserts

CDK12 (CDK12 Human)
CCNK (CCNK Human)

Use

Tags

Flag-3C

Expression

Insect

Mutation

CDK12(700-1082)

Promoter

Available since

May 2, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pBIG1a_GST-TEV-CK2alpha'_CK2beta

Plasmid

#223741

Purpose

Expression of recombinant protein for purification

Depositor

Sascha Martens

Article

Adriaenssens et al bioRxiv [Preprint]. 2024 Aug 28:2024.08.

Insert

CK2-alphaprime_CK2-beta (CSNK2A2 Human)

Use

Tags

GST-TEV

Expression

Insect

Mutation

Promoter

Available since

March 25, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pBiFC-SS-VC155-IRE1-dLD

Plasmid

#217751

Purpose

Expresses the VC fragment of BiFC-IRE1-dLD

Depositor

Christina Chan

Article

Cho et al Cell Rep. 2019 Apr 2;27(1):307-320.e5. d

Insert

VC155-IRE1-dLD (ERN1 Human)

Use

Tags

Venus (155-238, A206K)

Expression

Mammalian

Mutation

deleted amino acids 29-408

Promoter

Available since

Jan. 24, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pBiT3.1-C_WDR81:1619-1941

Plasmid

#211047

Purpose

Mammalian expression of human WDR81 fragment A1619 to A1941. C-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

WDR81:A1619-A1941 (WDR81 Human)

Use

Tags

GSSGGSSGVSGWRLFKKIS

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available since

March 20, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pBiT3.1-N_LRRK2:2142-2527

Plasmid

#211112

Purpose

Mammalian expression of human LRRK2 fragment R2142 to E2527. N-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

LRRK2:R2142-E2527 (LRRK2 Human)

Use

Tags

MVSGWRLFKKISGSSGGSSG

Expression

Mammalian

Mutation

M2397T natural variant (VAR_024965)

Promoter

CMV

Available since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge