We narrowed to 41,719 results for: Eng

1. pAAV-hSyn1-tTA

   Plasmid

   #99119

   Purpose

   An AAV genome that expresses tTA from the hSyn1 promoter

   Depositor

   Viviana Gradinaru

   Article

   Chan et al Nat Neurosci. 2017 Aug;20(8):1172-1179.

   Insert

   tTA
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   *(see below)

   Promoter

   hSyn1

   Available since

   Sept. 15, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHAGE DN Cullin5

   Plasmid

   #41916

   Depositor

   Stephen Elledge

   Article

   Emanuele et al Cell. 2011 Oct 14;147(2):459-74. doi: 10

   Insert

   CUL5 (CUL5 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 3, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pJL-SaKKH-BE3

   Plasmid

   #85170

   Purpose

   Expresses SaKKH-BE3 in mammalian cells [Edit]

   Depositor

   David Liu

   Article

   Kim et al Nat Biotechnol. 2017 Feb 13. doi: 10.103

   Insert

   SaKKH-BE3
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. MSCV-miRE_shBRD9_561-SV40-GFP

   Plasmid

   #75139

   Purpose

   Retroviral expression plasmid encoding shBRD9_561 (targeting human BRD)

   Depositor

   Christopher Vakoc

   Article

   Hohmann et al Nat Chem Biol. 2016 Jul 4. doi: 10.1038/

   Insert

   shBRD9_561
   

   Use

   Retroviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 23, 2016

   Availability

   Academic Institutions and Nonprofits only

5. pFGAMS-mEos2

   Plasmid

   #105128

   Purpose

   Expresses human FGAMS-mEos2 fusion in mammalian cells

   Depositor

   Stephen Benkovic

   Article

   French et al Science. 2016 Feb 12;351(6274):733-7. do

   Insert

   PFAS (PFAS Human)
   

   Use

   Tags

   mEos2

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDD287 (mKate2^SEC^3xMyc

   Plasmid

   #70685

   Purpose

   mKate2^SEC^3xMyc vector with ccdB sites for cloning homology arms

   Depositor

   Bob Goldstein

   Article

   Dickinson et al Genetics. 2015 Jun 3. pii: genetics.115.

   Insert

   mKate2-C1^SEC^3xFlag
   

   Use

   CRISPR and Cre/Lox

   Tags

   3xMyc and C. elegans codon-optimized mKate2

   Expression

   Worm

   Mutation

   Promoter

   Available since

   Jan. 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pX330A_D10A-1x5

   Plasmid

   #58775

   Purpose

   Expresses Cas9 nickase and gRNA

   Depositor

   Takashi Yamamoto

   Article

   Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103

   Insert

   humanized S. pyogenes Cas9 (D10A) nickase
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   D10A nickase-converting mutation

   Promoter

   CBh

   Available since

   Nov. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCDNA3 Flag Jnk1a1

   Plasmid

   #13798

   Depositor

   Roger Davis

   Article

   Derijard et al Cell. 1994 Mar 25. 76(6):1025-37.

   Insert

   Jnk1a1 (MAPK8 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 22, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. eGFP STAT1 Y701F

   Plasmid

   #12302

   Depositor

   Alan Perantoni

   Article

   Timofeeva et al Oncogene. 2006 Jun 26. ():.

   Insert

   STAT1 Y701F (STAT1 Human)
   

   Use

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   Y701F

   Promoter

   Available since

   Sept. 1, 2006

   Availability

   Academic Institutions and Nonprofits only

10. pCDNA3-myc3-ROC1

    Plasmid

    #20717

    Depositor

    Yue Xiong

    Article

    He et al Genes Dev. 2006 Nov 1. 20(21):2949-54.

    Insert

    RING finger protein 1 (ROC1) (RBX1 Human)
    

    Use

    Tags

    3xMyc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 31, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pAAV-CAG-tTA

    Plasmid

    #99118

    Purpose

    An AAV genome that ubiquitously expresses tTA from the CAG promoter

    Depositor

    Viviana Gradinaru

    Article

    Chan et al Nat Neurosci. 2017 Aug;20(8):1172-1179.

    Has Service

    AAV2

    Insert

    tTA
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    *(see below)

    Promoter

    CAG

    Available since

    Sept. 15, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLV hUbC-VP64 dCas9 VP64-T2A-GFP

    Plasmid

    #59791

    Purpose

    Co-expresses human optimized S. pyogenes dCas9 fused to two copies of VP64 and GFP

    Depositor

    Charles Gersbach

    Article

    Kabadi et al Nucleic Acids Res. 2014 Aug 13. pii: gku

    Insert

    humanized VP64 dead Cas9 VP64 T2A GFP
    

    Use

    CRISPR and Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    D10A and H840A

    Promoter

    Available since

    Oct. 23, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. p46Cpf1-OP2

    Plasmid

    #98592

    Purpose

    Produces lambda Red and Cpf1 for recombination and selection. The plasmid is combined with a donor plasmid (with crRNA and template) for rapid genome editing in E.coli.

    Depositor

    Qiong Wu

    Article

    Ao et al Front Microbiol. 2018 Oct 9;9:2307. doi:

    Insert

    FnCpf1
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Codon optimized for E. coli

    Promoter

    araBAD

    Available since

    Feb. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pKD237-3a-2

    Plasmid

    #90957

    Purpose

    Expresses ThsR and mCherry constitutively and sfGFP under the PphsA promoter

    Depositor

    Jeffrey Tabor

    Article

    Daeffler et al Mol Syst Biol. 2017 Apr 3;13(4):923. doi

    Inserts

    Thiosulfate response regulator
    Superfolder GFP
    mCherry
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Bba_J23100, Bba_J23105, and PphsA

    Available since

    May 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. Cerulean-2xFv-hRIPK3

    Plasmid

    #106079

    Purpose

    expression in mammalian cells

    Depositor

    Douglas Green

    Article

    Quarato et al Mol Cell. 2016 Feb 18;61(4):589-601. doi

    Insert

    Cerulean-2xFv-hRIPK3 (RIPK3 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    LTR

    Available since

    March 1, 2018

    Availability

    Academic Institutions and Nonprofits only

16. pAAV.CAG.GFPsm-myc.WPRE.SV40

    Plasmid

    #98926

    Purpose

    AAV expression of GFP spaghetti monster myc construct

    Depositor

    Loren Looger

    Article

    Viswanathan et al Nat Methods. 2015 Jun;12(6):568-76. doi:

    Insert

    GFP-smFP-myc
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CBA

    Available since

    Jan. 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. FLHKII-pGFPN3

    Plasmid

    #21920

    Depositor

    Hossein Ardehali

    Article

    Sun et al Mol Cell Biol. 2008 Feb . 28(3):1007-17.

    Insert

    Full length human HKII cDNA in pGFP-N3 plasmid (HK2 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    This is the full length human HKII cDNA sequence …

    Promoter

    Available since

    Sept. 17, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAAV-hSyn1-mRuby2

    Plasmid

    #99126

    Purpose

    An AAV genome that expresses the fluorescent protein mRuby2 from the hSyn1 promoter

    Depositor

    Viviana Gradinaru

    Article

    Chan et al Nat Neurosci. 2017 Aug;20(8):1172-1179.

    Insert

    mRuby2
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hSyn1

    Available since

    Aug. 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAAV-TRE-mNeonGreen

    Plasmid

    #99131

    Purpose

    An AAV genome with tet-inducible expression of the fluorescent protein mNeonGreen

    Depositor

    Viviana Gradinaru

    Article

    Chan et al Nat Neurosci. 2017 Aug;20(8):1172-1179.

    Insert

    mNeonGreen
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE

    Available since

    March 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA3-E2F4

    Plasmid

    #10914

    Depositor

    Bob Weinberg

    Article

    Sardet et al Proc Natl Acad Sci U S A. 1995 Mar 14. 9

    Insert

    E2F4 (E2F4 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 11, 2005

    Availability

    Academic Institutions and Nonprofits only

21. pAAV-TRE-mRuby2

    Plasmid

    #99114

    Purpose

    An AAV genome with tet-inducible expression of the fluorescent protein mRuby2

    Depositor

    Viviana Gradinaru

    Article

    Chan et al Nat Neurosci. 2017 Aug;20(8):1172-1179.

    Insert

    mRuby2
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE

    Available since

    Aug. 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pAV126

    Plasmid

    #63192

    Purpose

    pRS426 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG.

    Depositor

    Jef Boeke

    Article

    Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    Aug. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pLZ31 (pCFJ151_Peft-3_TIR1_linker_mRuby_unc-54 3'UTR)

    Plasmid

    #71720

    Purpose

    Expressing an mRuby-tagged TIR1 in the soma of C. elegans

    Depositor

    Abby Dernburg

    Article

    Zhang et al Development. 2015 Nov 9. pii: dev.129635

    Insert

    TRANSPORT INHIBITOR RESPONSE 1 (TIR1 Mustard Weed)
    

    Use

    Tags

    mRuby

    Expression

    Worm

    Mutation

    codon-optimized for expression in C. elegans; Two…

    Promoter

    eef-1A.1 (eft-3)

    Available since

    Dec. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. 3XFlagNICD3

    Plasmid

    #20185

    Depositor

    Raphael Kopan

    Article

    Ong et al J Biol Chem. 2006 Feb 24. 281(8):5106-19

    Insert

    Notch 3 Intracellular Domain (Notch3 Mouse)
    

    Use

    Tags

    3X Flag

    Expression

    Mammalian

    Mutation

    Contains murine Notch 3 Intracellular Domain (Met…

    Promoter

    CMV

    Available since

    Dec. 13, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pAV116

    Plasmid

    #63183

    Purpose

    pRS416 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG.

    Depositor

    Jef Boeke

    Article

    Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    Sept. 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pRK5 FLAG metap2

    Plasmid

    #32004

    Depositor

    David Sabatini

    Article

    Peterson et al Cell. 2011 Aug 5;146(3):408-20.

    Insert

    metap2 (Metap2 )
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, SP6

    Available since

    Sept. 27, 2011

    Availability

    Academic Institutions and Nonprofits only

27. HA-Daxx/pRK5

    Plasmid

    #52023

    Purpose

    Expresses HA-Daxx in mammalian cells

    Depositor

    Xiaolu Yang

    Article

    Tang et al PLoS One. 2013;8(2):e55813. doi: 10.1371

    Insert

    HA-Daxx (DAXX Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pRVL-5

    Plasmid

    #104583

    Purpose

    Contains human IgG1 CH1/CH2/CH3 domains, for cloning of antibody VH domains using SapI restriction enzyme. Full Fc-mediated immune effector functions

    Depositor

    Daniel Christ

    Article

    Vazquez-Lombardi et al Methods Mol Biol. 2018;1827:313-334. doi

    Insert

    Human IgG1 CH1/CH2/CH3 (IGHG1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pCVL Traffic Light Reporter 1.1 (Sce target) Ef1a BFP

    Plasmid

    #31481

    Depositor

    Andrew Scharenberg

    Article

    Certo et al Nat Methods. 2011 Jul 10;8(8):671-6. doi

    Insert

    Traffic Light Reporter 1.1 (Sce target) EF BFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    mCherry contains M9S and M16L to reduce backgroun…

    Promoter

    Available since

    Aug. 22, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pBK-VQR-BE3

    Plasmid

    #85171

    Purpose

    Expresses VQR-BE3 in mammalian cells

    Depositor

    David Liu

    Article

    Kim et al Nat Biotechnol. 2017 Feb 13. doi: 10.103

    Insert

    VQR-BE3
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge