We narrowed to 9,178 results for: mel

1. pPB-cT3G-cERP2-GATA4

   Plasmid

   #192896

   Purpose

   Barcoded piggybac transposon vector with Dox-inducible expression of GATA4

   Depositor

   George Church

   Article

   Pierson Smela et al Elife. 2023 Feb 21;12:e83291. doi: 10.75

   Insert

   GATA4 (GATA4 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Codon-optimized

   Promoter

   TET3G-miniCMV

   Available Since

   Feb. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHDR-FOXL2-T2A-tdTomato-PuroTK

   Plasmid

   #192892

   Purpose

   HDR donor for inserting FOXL2-T2A-tdTomato reporter

   Depositor

   George Church

   Article

   Pierson Smela et al Elife. 2023 Feb 21;12:e83291. doi: 10.75

   Insert

   FOXL2-tdTomato (FOXL2 Human)
   

   Use

   CRISPR

   Mutation

   T2A-tdTomato fluorescent reporter

   Promoter

   PGK

   Available Since

   Feb. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pPB-cT3G-cERP2-EGR1

   Plasmid

   #192913

   Purpose

   Barcoded piggybac transposon vector with Dox-inducible expression of EGR1

   Depositor

   George Church

   Article

   Pierson Smela et al Elife. 2023 Feb 21;12:e83291. doi: 10.75

   Insert

   EGR1 (EGR1 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Promoter

   TET3G-miniCMV

   Available Since

   Feb. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pLX313-TP53-P278A

   Plasmid

   #118015

   Purpose

   Constitutive expression of mutant human Tumor Protein p53 (TP53)

   Depositor

   William Hahn, David Root

   Article

   Giacomelli et al Nat Genet. 2018 Oct;50(10):1381-1387. do

   Insert

   TP53 (TP53 Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   p.Pro278Ala

   Promoter

   EF1alpha

   Available Since

   Oct. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pPB-cT3G-cERP2-CEBPD

   Plasmid

   #192897

   Purpose

   Barcoded piggybac transposon vector with Dox-inducible expression of CEBPD

   Depositor

   George Church

   Article

   Pierson Smela et al Elife. 2023 Feb 21;12:e83291. doi: 10.75

   Insert

   CEBPD (CEBPD Human)
   

   Expression

   Mammalian

   Promoter

   TET3G-miniCMV

   Available Since

   Feb. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. MEK1 (S218D,S222D)-pcw107

   Plasmid

   #64604

   Purpose

   when used to produce lentivirus, express physiological levels of insert

   Depositor

   David Sabatini, Kris Wood

   Article

   Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

   Insert

   MAP2K1 (MAP2K1 Human)
   

   Use

   Lentiviral

   Mutation

   S218D, S222D

   Promoter

   PGK

   Available Since

   June 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLentiCMVblast_SLC38A2_wt

   Plasmid

   #156180

   Purpose

   CMV-driven expression of sgRNA-resistant SLC38A2 wild-type cDNA.

   Depositor

   Alec Kimmelman

   Article

   Parker et al Cancer Discov. 2020 Apr 27. pii: 2159-82

   Insert

   SLC38A2 (SLC38A2 Human)
   

   Expression

   Mammalian

   Mutation

   Silent mutations to prevent targeting by sgRNA se…

   Promoter

   CMV

   Available Since

   Sept. 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pINDUCER20_mStrawberry_Atg4BC74A

   Plasmid

   #161735

   Purpose

   Doxycycline-inducible expression of Atg4B(C74A) fused with mStrawberry to inhibit autophagy

   Depositor

   Alec Kimmelman

   Article

   Yamamoto et al Nature. 2020 May;581(7806):100-105. doi:

   Insert

   Atg4B(C74A) (Atg4b Mouse) (Atg4b Mouse)
   

   Use

   Lentiviral; Destinatioin vector for gateway cloni…

   Tags

   mStrawberry

   Expression

   Mammalian

   Mutation

   220-222 TGC (Cys) is changed to GCA (Ala)

   Available Since

   Dec. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pIGF1_P1 Promoter-FLuc

   Plasmid

   #154266

   Purpose

   Firefly luciferase reporter containing the canonical Human IGF-1 P1 Promoter.

   Depositor

   Antonio Amelio

   Article

   Musicant et al Cell Rep. 2021 Feb 23;34(8):108768. doi:

   Insert

   IGF-1 canonical P1 Promoter (IGF1 Human)
   

   Expression

   Mammalian

   Promoter

   Human IGF-1 P1 Promoter

   Available Since

   March 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pXPR003-sgTP53-2

    Plasmid

    #118020

    Purpose

    Constitutive expression of sgRNA targeting human TP53

    Depositor

    William Hahn, David Root

    Article

    Giacomelli et al Nat Genet. 2018 Oct;50(10):1381-1387. do

    Insert

    sgTP53-2 (TP53 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    Oct. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pXPR003-sgTP53-4

    Plasmid

    #118022

    Purpose

    Constitutive expression of sgRNA targeting human TP53

    Depositor

    William Hahn, David Root

    Article

    Giacomelli et al Nat Genet. 2018 Oct;50(10):1381-1387. do

    Insert

    sgTP53-4 (TP53 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    Oct. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pXPR003-sgTP53-1

    Plasmid

    #118019

    Purpose

    Constitutive expression of sgRNA targeting human TP53

    Depositor

    William Hahn, David Root

    Article

    Giacomelli et al Nat Genet. 2018 Oct;50(10):1381-1387. do

    Insert

    sgTP53-1 (TP53 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    Oct. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pPB-cT3G-cERP2-HOPX

    Plasmid

    #192923

    Purpose

    Barcoded piggybac transposon vector with Dox-inducible expression of HOPX

    Depositor

    George Church

    Article

    Pierson Smela et al Elife. 2023 Feb 21;12:e83291. doi: 10.75

    Insert

    HOPX (HOPX Human)
    

    Tags

    HA

    Expression

    Mammalian

    Promoter

    TET3G-miniCMV

    Available Since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pXPR003-sgTP53-3

    Plasmid

    #118021

    Purpose

    Constitutive expression of sgRNA targeting human TP53

    Depositor

    William Hahn, David Root

    Article

    Giacomelli et al Nat Genet. 2018 Oct;50(10):1381-1387. do

    Insert

    sgTP53-3 (TP53 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    Oct. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pSLIK_Hyg_mTurquoise2_Atg4BC74A

    Plasmid

    #161733

    Purpose

    Doxycycline-inducible expression of Atg4B(C74A) fused with mTurquoise2 to inhibit autophagy

    Depositor

    Alec Kimmelman

    Article

    Yamamoto et al Nature. 2020 May;581(7806):100-105. doi:

    Insert

    Atg4B(C74A) (Atg4b Mouse) (Atg4b Mouse)
    

    Use

    Lentiviral; Destinatioin vector for gateway cloni…

    Tags

    mTurquoise2

    Expression

    Mammalian

    Mutation

    220-222 TGC (Cys) is changed to GCA (Ala)

    Available Since

    Nov. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pPB-cT3G-cERP2-KLF6

    Plasmid

    #192909

    Purpose

    Barcoded piggybac transposon vector with Dox-inducible expression of KLF6

    Depositor

    George Church

    Article

    Pierson Smela et al Elife. 2023 Feb 21;12:e83291. doi: 10.75

    Insert

    KLF6 (KLF6 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Promoter

    TET3G-miniCMV

    Available Since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. MEK1-pcw107-V5

    Plasmid

    #64650

    Purpose

    (control) when used to produce lentivirus, express physiological levels of insert

    Depositor

    David Sabatini, Kris Wood

    Article

    Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

    Insert

    MAP2K1 (MAP2K1 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Promoter

    PGK

    Available Since

    Dec. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

18. pXPR003-sgTP53-5

    Plasmid

    #118023

    Purpose

    Constitutive expression of sgRNA targeting human TP53

    Depositor

    William Hahn, David Root

    Article

    Giacomelli et al Nat Genet. 2018 Oct;50(10):1381-1387. do

    Insert

    sgTP53-5 (TP53 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    Oct. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pInducer20_SLC38A2_wt

    Plasmid

    #156182

    Purpose

    Dox-inducible expression of sgRNA-resistant SLC38A2 wild-type cDNA.

    Depositor

    Alec Kimmelman

    Article

    Parker et al Cancer Discov. 2020 Apr 27. pii: 2159-82

    Insert

    SLC38A2 (SLC38A2 Human)
    

    Expression

    Mammalian

    Mutation

    Silent mutations to prevent targeting by sgRNA: T…

    Promoter

    TRE

    Available Since

    Aug. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pIGF1_P2 Promoter-FLuc

    Plasmid

    #154267

    Purpose

    Firefly luciferase reporter containing the downstream Human IGF-1 P2 Promoter.

    Depositor

    Antonio Amelio

    Article

    Musicant et al Cell Rep. 2021 Feb 23;34(8):108768. doi:

    Insert

    IGF-1 P2 Promoter (IGF1 Human)
    

    Expression

    Mammalian

    Promoter

    Human IGF-1 P2 Promoter

    Available Since

    March 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge