We narrowed to 7,124 results for: nls

1. pSimpleII-NmCas9-FLAG

   Plasmid

   #47872

   Purpose

   FLAG tagged NmCas9 with no NLS.

   Depositor

   James Thomson, Erik Sontheimer

   Article

   Hou et al Proc Natl Acad Sci U S A. 2013 Aug 12.

   Insert

   NmCas9
   

   Use

   CRISPR

   Tags

   FLAG

   Expression

   Mammalian

   Available Since

   Sept. 25, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pAIO-LbCpf1

   Plasmid

   #137847

   Purpose

   LbCpf1 and gRNA expression plasmid for P. falciparum; no selection in parasites.

   Depositor

   Josh Beck

   Article

   Nessel et al Cell Microbiol. 2020 Jan 28:e13168. doi:

   Insert

   LbCpf1-NLS-FLAG
   

   Use

   CRISPR

   Available Since

   April 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pAC1748

   Plasmid

   #127982

   Purpose

   AMA1 plasmid with Aspergillus optimized Cpf1, argB selection marker, and USER cassette (PacI/Nt.BbvCI)

   Depositor

   Uffe Mortensen

   Article

   Vanegas et al Fungal Biol Biotechnol. 2019 May 1;6:6.

   Insert

   Cpf1
   

   Use

   CRISPR; Fungal expression

   Tags

   SV40 NLS

   Expression

   Bacterial

   Mutation

   codon optimized for Aspergillus nidulans

   Promoter

   Aspergillus nidulans tef1 promoter

   Available Since

   Dec. 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pTX172

   Plasmid

   #89259

   Purpose

   Express STU (Single Transcriptional Unit) Cas9 with Maize Ubiquitin1 promoter in plants

   Depositor

   Daniel Voytas

   Article

   Tang et al Mol Plant. 2016 Jul 6;9(7):1088-91. doi:

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   SV40 NLS

   Expression

   Plant

   Available Since

   May 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pTX176

   Plasmid

   #89260

   Purpose

   Express estrodial-inducible STU (Single Transcriptional Unit) Cas9 in plants

   Depositor

   Daniel Voytas

   Article

   Tang et al Mol Plant. 2016 Jul 6;9(7):1088-91. doi:

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   SV40 NLS

   Expression

   Plant

   Available Since

   May 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pAT15001-AncBE4max

   Plasmid

   #163016

   Purpose

   plasmid expressing AncBE4max

   Depositor

   Ervin Welker

   Article

   Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

   Insert

   AncBE4max
   

   Use

   CRISPR

   Tags

   3xFLAG and SV40 NLS

   Expression

   Mammalian

   Available Since

   Sept. 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. p1148-pCSDest-AcrIIA4-mCherry

   Plasmid

   #126204

   Purpose

   Mammalian expression of AcrIIA4 fused to mCherry

   Depositor

   Erik Sontheimer

   Article

   Lee et al RNA. 2019 Aug 22. pii: rna.071704.119. d

   Insert

   codon-optimized AcrIIA4
   

   Tags

   FLAG/NLS and mCherry

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pLV-REPORT(EF1a no GFP) mnuctdTomato

   Plasmid

   #172342

   Purpose

   mnuctdTomato

   Depositor

   David Bersten

   Article

   Allen et al bioRxiv 2023.11.21.568191

   Inserts

   monomeric nuclear Tomato
   HygR
   

   Use

   Lentiviral and Synthetic Biology

   Tags

   SV40 NLS

   Expression

   Mammalian

   Promoter

   EF1alpha

   Available Since

   June 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pAIO-AsCpf1

   Plasmid

   #137846

   Purpose

   AsCpf1 and gRNA expression plasmid for P. falciparum; no selection in parasites.

   Depositor

   Josh Beck

   Article

   Nessel et al Cell Microbiol. 2020 Jan 28:e13168. doi:

   Insert

   AsCpf1-NLS-FLAG
   

   Use

   CRISPR

   Available Since

   April 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pQP-AR10

    Plasmid

    #138980

    Purpose

    Endogenous RAS regulation using light-controlled intrabody

    Depositor

    Vladislav Verkhusha

    Article

    Redchuk et al Nat Commun. 2020 Jan 30;11(1):605. doi:

    Insert

    BglII-BphP1-NES-T2A- iB(RAS)-NES-mCherry-QPAS1- NLS-NotI
    

    Available Since

    April 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pTX168

    Plasmid

    #89257

    Purpose

    Express STU (Single Transcriptional Unit) Cas9 with CaMV 35s promoter in plants

    Depositor

    Daniel Voytas

    Article

    Tang et al Mol Plant. 2016 Jul 6;9(7):1088-91. doi:

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    SV40 NLS

    Expression

    Plant

    Available Since

    May 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pYPQ159

    Plasmid

    #69308

    Purpose

    Gateway entry vector with hSpCas9D10A

    Depositor

    Yiping Qi

    Article

    Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

    Insert

    hSpCas9D10A (Human codon optimized)
    

    Use

    CRISPR

    Tags

    3XFLAG

    Mutation

    D10A mutation; NLS fusion

    Available Since

    Nov. 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pAT9749-dABE

    Plasmid

    #162998

    Purpose

    plasmid expressing ABE with dCas9

    Depositor

    Ervin Welker

    Article

    Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

    Insert

    dABE
    

    Use

    CRISPR

    Tags

    3xFLAG and SV40 NLS

    Expression

    Mammalian

    Promoter

    EF1-a

    Available Since

    Sept. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pRG711_lexO-AscI_LexA-TF_HIS3MX

    Plasmid

    #154820

    Purpose

    Inducible expression of AscI endonuclease in S. cerevisiae

    Depositor

    Lorraine Symington

    Article

    Gnugge et al Nucleic Acids Res. 2020 Oct 14. pii: 592

    Inserts

    AscI endonuclease
    LexA-ER-B112
    

    Tags

    FLAG and NLS

    Expression

    Yeast

    Promoter

    ACT1 and lexO_4-CYC1_core

    Available Since

    Oct. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pAT15002-ABEmax

    Plasmid

    #163006

    Purpose

    plasmid expressing ABEmax

    Depositor

    Ervin Welker

    Article

    Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

    Insert

    ABEmax
    

    Use

    CRISPR

    Tags

    SV40 NLS

    Expression

    Mammalian

    Promoter

    EF1-a

    Available Since

    Sept. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pAT15064-eCBE

    Plasmid

    #163009

    Purpose

    plasmid expressing eCBE

    Depositor

    Ervin Welker

    Article

    Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

    Insert

    eCBE
    

    Use

    CRISPR

    Tags

    3xFLAG and SV40 NLS

    Expression

    Mammalian

    Promoter

    EF1-a

    Available Since

    Sept. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAT9676-ABE

    Plasmid

    #162997

    Purpose

    plasmid expressing ABE

    Depositor

    Ervin Welker

    Article

    Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

    Insert

    ABE
    

    Use

    CRISPR

    Tags

    3xFLAG and SV40 NLS

    Expression

    Mammalian

    Promoter

    EF1-a

    Available Since

    Sept. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. PABE-2

    Plasmid

    #115623

    Purpose

    Targeted A to G in wheat plants or monocotyledons protoplasts

    Depositor

    Caixia Gao

    Article

    Li et al Genome Biol. 2018 May 29;19(1):59. doi:

    Insert

    wtTadA-TadA7.10-nCas9-NLS
    

    Use

    CRISPR

    Expression

    Plant

    Available Since

    Jan. 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pBALU9

    Plasmid

    #69324

    Purpose

    Encodes a recombineering cassette for tagging a target gene within a genomic clone with a fluorescent protein. Cassette: bicistronic; FRT-galK-FRT in 2nd GFP intron;use C-term after STOP

    Depositor

    Oliver Hobert

    Article

    Tursun et al PLoS One. 2009;4(3):e4625. doi: 10.1371/

    Insert

    gSL2-NLS-GFPint-FgF
    

    Expression

    Bacterial

    Available Since

    Sept. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

20. pYPQ158

    Plasmid

    #69307

    Purpose

    Gateway entry vector with hSpCas9 (Human codon optimized)

    Depositor

    Yiping Qi

    Article

    Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

    Insert

    hSpCas9 (Human codon optimized)
    

    Use

    CRISPR

    Tags

    3XFLAG

    Mutation

    NLS fusion

    Available Since

    Oct. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge