We narrowed to 9,001 results for: ef1;

1. pAAV-LAP1_2-mCherry

   Plasmid

   #159526

   Purpose

   Expresses mCherry from LAP1_2 promoter

   Depositor

   Esteban Engel

   Article

   Maturana et al Mol Ther Methods Clin Dev. 2020 Apr 14;1

   Insert

   mCherry
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   LAP1_2

   Available since

   Sept. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pUDE1084

   Plasmid

   #168500

   Purpose

   expression of T7RNA polymerase and CRISPR endonunclease Fncas12a

   Depositor

   Jean-Marc Daran

   Article

   Randazzo et al CRISPR J. 2021 Apr 23. doi: 10.1089/cris

   Inserts

   Fncas12a
   T7RNA polymerase
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   TDH3 and TEF1

   Available since

   Dec. 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. HiFi dCas9 KRAB

   Plasmid

   #188500

   Purpose

   Expresses FLAG tagged HiFi dCas9 KRAB

   Depositor

   Isaac Hilton

   Article

   Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

   Insert

   dCas9
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   D10A/R691A/H840A

   Promoter

   EF1a

   Available since

   Sept. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. PB-CT3G-ERP2-MG-IRES2-mNeonGreen

   Plasmid

   #175506

   Purpose

   All-in-one piggyBac transposon destination vector for mCMV+dox-inducible expression of Mega Gate cloned elements upstream of IRES2-mNeonGreen (hEF1a-driven rtTA and puromycin resistance)

   Depositor

   George Church

   Article

   Kramme et al Cell Rep Methods. 2021 Sep 27;1(6):10008

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TRE3G-mCMV

   Available since

   Oct. 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHH0103_PDCD6_EF-hand

   Plasmid

   #110009

   Purpose

   Protein expression and purification of PDCD6_EF-hand

   Depositor

   Sachdev Sidhu

   Article

   Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

   Insert

   PDCD6_EF-hand (PDCD6 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-FDIO-dAPEX2

   Plasmid

   #117175

   Purpose

   Peroxidase reporter for multiplexed electron microscopy labeling

   Depositor

   David Ginty

   Article

   Zhang et al Nat Neurosci. 2019 Mar 18. pii: 10.1038/

   Insert

   dAPEX2
   

   Use

   AAV; Flp/frt

   Tags

   Expression

   Mammalian

   Mutation

   W41F and A134P on soybean APX

   Promoter

   EF1a

   Available since

   March 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. FH-TET3-HxDmut-pEF

   Plasmid

   #127896

   Purpose

   Expresses ten-eleven-translocation 3 (TET3) containing HxD mutation at H1077Y and D1079A

   Depositor

   Anjana Rao, Patrick Hogan

   Article

   Ko et al Nature. 2013 May 2;497(7447):122-6. doi:

   Insert

   TET3-HxDmut (TET3 Human)
   

   Use

   Tags

   HA and Flag

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1a

   Available since

   Oct. 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pPMVAd36

   Plasmid

   #98302

   Purpose

   Enhancing the lower-part mevalonate pathway; overexpressing yeast mevalonate pathway genes under the control of consitutive promoters or CUP1 promoter.

   Depositor

   Claudia Vickers

   Article

   Peng et al Biotechnol Biofuels. 2017 Feb 21;10:43.

   Insert

   PCUP1>ERG12>TNAT5-PTEF2>ERG8>TIDP1-TIDP1

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Aug. 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRPL28(K55M) SpHIS5 (pNTI666)

   Plasmid

   #115436

   Purpose

   Expresses K55M RPL28 from HIS3 yeast plasmid

   Depositor

   Nicholas Ingolia

   Article

   McGeachy et al ACS Synth Biol. 2019 Apr 19;8(4):844-856

   Inserts

   RPL28 (RPL28 Budding Yeast)
   HIS5
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   K55M; MfeI site in intron

   Promoter

   Ashbya gospii TEF1 and endogenous

   Available since

   Oct. 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. p413TEF-SirT1

    Plasmid

    #51746

    Purpose

    overexpression of human SirT1 in S. cerevisiae, HIS3 marker

    Depositor

    Markus Ralser

    Article

    Ralser et al PLoS One. 2009;4(4):e5376. doi: 10.1371/

    Insert

    SirT1 (SIRT1 Human)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TEF1

    Available since

    March 21, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. FH-TET3-CXXCmut-pEF

    Plasmid

    #127895

    Purpose

    Expresses ten-eleven-translocation 3 (TET3) containing CXXC mutation 80THQ82 to AAA

    Depositor

    Anjana Rao, Patrick Hogan

    Article

    Ko et al Nature. 2013 May 2;497(7447):122-6. doi:

    Insert

    TET3-CxxCmut (TET3 Human)
    

    Use

    Tags

    HA and Flag

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a

    Available since

    Oct. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pYSD153

    Plasmid

    #212929

    Purpose

    Encodes the mRuby2 fluorescent protein (Part3b') with the 111AA SCW4 TFP (Part3a') assembled with S. cerevisiae pTEF1 promoter, tTDH1 terminator, LEU2 selectable marker and CEN/ARS yeast origin.

    Depositor

    Paul Young

    Article

    O'Riordan et al ACS Synth Biol. 2024 Mar 14. doi: 10.102

    Insert

    mRuby2
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pYSD141

    Plasmid

    #212928

    Purpose

    Encodes the mRuby2 fluorescent protein (Part3b') with the Aga2 signal peptide (Part3a') assembled with S. cerevisiae pTEF1 promoter, tTDH1 terminator, LEU2 selectable marker and CEN/ARS yeast origin.

    Depositor

    Paul Young

    Article

    O'Riordan et al ACS Synth Biol. 2024 Mar 14. doi: 10.102

    Insert

    mRuby2
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCT

    Plasmid

    #60620

    Purpose

    Plasmid encoding iCas9 and tracrRNA on pRS415 backbone

    Depositor

    Huimin Zhao

    Article

    Bao et al ACS Synth Biol. 2014 Sep 19.

    Inserts

    iCas9
    tracrRNA
    

    Use

    CRISPR

    Tags

    FLAG and SV40 NLS

    Expression

    Yeast

    Mutation

    changed Aspartate 147 to Tyrosine, Proline 411 to…

    Promoter

    RPR1p and TEF1p

    Available since

    Nov. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCR1063

    Plasmid

    #111091

    Purpose

    Expresses dCas9-KRAB tagged with mCherry: pHR-hEF1a-dCas9-mcherry-KRAB-mPGK-Hyg

    Depositor

    Jacob Corn

    Article

    Richardson et al Nat Genet. 2018 Aug;50(8):1132-1139. doi

    Insert

    dCas9-KRAB
    

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Mutation

    Promoter

    Available since

    Oct. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3.1+N-eGFP_SAMD9L_R986C

    Plasmid

    #206176

    Purpose

    mammalian expression of SAMD9L R986C

    Depositor

    Christopher Goodnow

    Article

    Russell et al Proc Natl Acad Sci U S A. 2021 Aug 24;11

    Insert

    SAMD9L (SAMD9L Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    R986C

    Promoter

    CMV

    Available since

    Nov. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. PlmdCas12e KRAB

    Plasmid

    #188506

    Purpose

    Expresses FLAG tagged PlmdCas12e KRAB

    Depositor

    Isaac Hilton

    Article

    Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

    Insert

    dCas12e
    

    Use

    CRISPR and Lentiviral

    Tags

    NLS-FLAG-KRAB-NLS

    Expression

    Mammalian

    Mutation

    D659A/E756A/D922A

    Promoter

    EF1a

    Available since

    Sept. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pHCas9-Nours

    Plasmid

    #107733

    Purpose

    Escherichia coli- S. cerevisiae shuttle plasmid harbors a Cas9 gene, a natMX6 and URA3 selection markers used in S. cerevisiae

    Depositor

    Sheng Yang

    Article

    Wang et al Biotechnol Bioeng. 2018 Sep 10. doi: 10.

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    SV40 NLS

    Expression

    Yeast

    Mutation

    Human optimized

    Promoter

    TEF1

    Available since

    Jan. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMG

    Plasmid

    #138264

    Purpose

    Yeast intergration vector for integration of dual reporter system at the URA3 locus. Dual reporter system contains constitutive mCherry and constitutive eGFP flanked by iCas9 sites.

    Depositor

    Xiao Wang

    Article

    Standage-Beier et al CRISPR J. 2019 Aug;2:209-222. doi: 10.10

    Inserts

    eGFP flanked by iCas9 sites
    mCherry
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Tef1

    Available since

    March 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pEF HA-TSAdwt

    Plasmid

    #84517

    Purpose

    Mammalian expression of TSAd

    Depositor

    Anne Spurkland

    Article

    Granum et al BMC Immunol. 2006 Jul 13;7:15.

    Insert

    TSAd (SH2D2A Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Nov. 8, 2016

    Availability

    Academic Institutions and Nonprofits only