Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

Filter By

We narrowed to 50,968 results for: ANG

Showing: 4241 - 4260 of 50968 results

  4. pAC147-pCR8-dCas9VP64

    Plasmid
    #48219
    Purpose
    dCas9VP64 on Gateway donor vector pCR8/GW/TOPO. Note: This is not for expression. It has to be transferred to a gateway destination vector for expression 
    Depositor
    Rudolf Jaenisch
    Article
    Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2
    Insert
    dCas9(D10A;H840A) fusion with VP64 activation domain
    Use
    CRISPR; Gateway donor
    Tags
    HA Tag and VP64
    Expression
    Mutation
    D10A;H840A
    Promoter
    Available since
    Sept. 17, 2013
    Availability
    Academic Institutions and Nonprofits only
    48219-plasmid-map-sequence-id-69504

    Enlarge

  5. pBT3035

    Plasmid
    #122543
    Purpose
    Expression of a C. elegan codon optimized florescent protein (CemOrange2) fused to a nuclear lamin localized gene (lmn-1) under the intestinal specific promoter nhx-2
    Depositor
    Gary Silverman
    Article
    Thomas et al PLoS One. 2019 Mar 26;14(3):e0214257. do
    Insert
    Pnhx-2_lmn-1::CemOrange2 (lmn-1 Nematode, Synthetic)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Pnhx-2
    Available since
    March 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    122543-plasmid-map-sequence-id-238214

    Enlarge

  12. pCAT526

    Plasmid
    #180513
    Purpose
    Plasmid expressing mammalian codon optimized wt PlmCasX, mNeonGreen, sgRNAv1 scaffold, and restriction sites to clone in new spacers
    Depositor
    Jennifer Doudna
    Article
    Tsuchida et al Mol Cell. 2022 Mar 17;82(6):1199-1209.e6
    Inserts
    CasX sgRNAv1
    PlmCasX-2A-mNeonGreen
    Use
    CRISPR
    Tags
    2x FLAG and SV40 NLS
    Expression
    Mammalian
    Mutation
    Promoter
    CAG and U6
    Available since
    April 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    180513-plasmid-map-sequence-id-359728

    Enlarge

  13. pAC148-pCR8-dCas9VP96

    Plasmid
    #48220
    Purpose
    dCas9VP96 on Gateway donor vector pCR8/GW/TOPO. Note: This is not for expression. It has to be transferred to a gateway destination vector for expression
    Depositor
    Rudolf Jaenisch
    Article
    Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2
    Insert
    dCas9(D10A;H840A) fusion with VP96 activation domain
    Use
    CRISPR; Gateway donor
    Tags
    HA Tag and VP96
    Expression
    Mutation
    D10A;H840A
    Promoter
    Available since
    Sept. 17, 2013
    Availability
    Academic Institutions and Nonprofits only
    48220-plasmid-map-sequence-id-69505

    Enlarge

  15. pUC19-F3UXG6

    Plasmid
    #103108
    Purpose
    Cas9 coding gene template with optimized sequence for human codon usage
    Depositor
    Duhee Bang
    Article
    Cho et al Nucleic Acids Res. 2018 May 18;46(9):e55
    Insert
    F3UXG6(Cas9 coding gene from Streptococcus sanguinis SK49)
    Use
    CRISPR; Cloning vector
    Tags
    Expression
    Mutation
    human codon-optimized
    Promoter
    Available since
    April 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    103108-plasmid-map-sequence-id-201817

    Enlarge

  17. pBT3102

    Plasmid
    #122548
    Purpose
    Expression of a C. elegan codon optimized florescent protein (CemOrange2) fused to a plasma membrane localized gene (aqp-1) under the intestinal specific promoter nhx-2
    Depositor
    Gary Silverman
    Article
    Thomas et al PLoS One. 2019 Mar 26;14(3):e0214257. do
    Insert
    Pnhx-2_aqp-1::CemOrange2 (aqp-1 Nematode, Synthetic)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Pnhx-2
    Available since
    March 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    122548-plasmid-map-sequence-id-238198

    Enlarge

  18. pBT2999

    Plasmid
    #122535
    Purpose
    Expression of a C. elegan codon optimized fluorescent protein (CemOrange2) fused to a lysosome localized gene (lmp-1) under the intestinal specific promoter nhx-2
    Depositor
    Gary Silverman
    Article
    Thomas et al PLoS One. 2019 Mar 26;14(3):e0214257. do
    Insert
    Pnhx-2_lmp-1::CemOrange2 (lmp-1 Nematode, Synthetic)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Pnhx-2
    Available since
    March 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    122535-plasmid-map-sequence-id-238208

    Enlarge

  19. pBT3047

    Plasmid
    #122547
    Purpose
    Expression of a C. elegan codon optimized florescent protein (CemOrange2) fused to a N-terminal (sv-40) and C-terminal (egl-13) nuclear localization signal under the intestinal specific promoter nhx-2
    Depositor
    Gary Silverman
    Article
    Thomas et al PLoS One. 2019 Mar 26;14(3):e0214257. do
    Insert
    Pnhx-2_NLS(sv-40)::CemOrange2::NLS(egl-13)
    Use
    Tags
    MAPKKKRKVV and MSRRRKANPTKLSENAKKLAKEVEN
    Expression
    Bacterial
    Mutation
    Promoter
    Pnhx-2
    Available since
    March 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    122547-plasmid-map-sequence-id-238211

    Enlarge

  20. pEJS2783 CMV-AcrIIC3-FLAGNLS-mCherry-3xmiR-16 CMV-BFP

    Plasmid
    #240513
    Purpose
    Mammalian expression of AcrIIC3-mCherry fusion with microRNA target sites in the 3' UTR as a reporter of microRNA repression. BFP expressed separately as a transfection control.
    Depositor
    Erik Sontheimer
    Article
    Kraus et al RNA. 2025 May 28:rna.080566.125. doi: 10
    Insert
    AcrIIC3
    Use
    Tags
    FLAG, hsa-miR-16 Target Sites, and mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Aug. 11, 2025
    Availability
    Academic Institutions and Nonprofits only
    240513-plasmid-map-sequence-id-477190

    Enlarge

Showing: 4241 - 4260 of 50968 results