Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

We narrowed to 6,978 results for: hwa

Showing: 4241 - 4260 of 6978 results

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PB-U6-CNCB_sgEIF3D-1

Plasmid

#236765

Purpose

A piggybac-based vector containing mouse U6 promoter-driven EIF3D sgRNA #1 and CAG promoter-driven nuclear-localized Clover-T2A-BSR.

Depositor

Kazutoshi Takahashi

Article

Okubo et al Sci Adv. 2025 Apr 11;11(15):eadq5484. do

Insert

EIF3D sgRNA-1 (EIF3D Human)

Use

Piggybac

Expression

Mammalian

Promoter

mouse U6 promoter and mouse U6 promoter, CAG prom…

Available Since

July 8, 2025

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(hXIAP_1k)-PGKpuro2ABFP-W

Plasmid

#208420

Purpose

Lentiviral gRNA plasmid targeting human XIAP gene, co-expression of BFP tag

Depositor

Sok Ching Cheong

Article

Chai et al Cancer Res Commun. 2024 Nov 1;4(11):2919

Insert

XIAP (XIAP Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

N/A

Promoter

human U6

Available Since

July 1, 2025

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(hXIAP_2b)-PGKpuro2ABFP-W

Plasmid

#208421

Purpose

Lentiviral gRNA plasmid targeting human XIAP gene, co-expression of BFP tag

Depositor

Sok Ching Cheong

Article

Chai et al Cancer Res Commun. 2024 Nov 1;4(11):2919

Insert

XIAP (XIAP Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

N/A

Promoter

human U6

Available Since

July 1, 2025

Availability

Academic Institutions and Nonprofits only

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pPB-EF1A-HALO-hLRRK2 (LIR1 mutant WEVL -> AEVA)

Plasmid

#229742

Purpose

Expresses Halo tagged human LRRK2 with LIR1 point mutations in mammalian cells. Stable expression with piggybac transposase.

Depositor

Shawn Ferguson

Article

Bentley-DeSousa et al J Cell Biol. 2025 Feb 3;224(2):e20231015

Insert

LRRK2 (LRRK2 Human)

Tags

Halo

Expression

Mammalian

Mutation

LIR1 mutant WEVL -> AEVA

Promoter

EF1A

Available Since

Feb. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pPB-EF1A-HALO-hLRRK2 (LIR2 mutant WTFI -> ATFA)

Plasmid

#229743

Purpose

Expresses Halo tagged human LRRK2 with LIR2 point mutations in mammalian cells. Stable expression with piggybac transposase.

Depositor

Shawn Ferguson

Article

Bentley-DeSousa et al J Cell Biol. 2025 Feb 3;224(2):e20231015

Insert

LRRK2 (LRRK2 Human)

Tags

Halo

Expression

Mammalian

Mutation

LIR2 mutant WTFI -> ATFA

Promoter

EF1A

Available Since

Feb. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pTag4C_SP-DRD2-V2R-NTEV-tcs-GV-2xHA

Plasmid

#227103

Purpose

DRD2 receptor for split TEV assay

Depositor

Michael Wehr

Article

Popovic et al Biomed Pharmacother. 2024 Nov;180:117523

Insert

DRD2-NTEV (DRD2 Human)

Tags

2xHA

Expression

Mammalian

Available Since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pET20b-mPTER-R204A

Plasmid

#222196

Purpose

Bacterial expression of mouse PTER-R204A protein

Depositor

Jonathan Long

Article

Wei et al bioRxiv [Preprint]. 2024 Mar 22:2024.03.

Insert

PTER (Pter Mouse)

Tags

6xHis and Strep

Expression

Bacterial

Mutation

R204A

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pNK7190

Plasmid

#219763

Purpose

MoClo-compatible Level M for expression nnLuz, nnH3H, nnCPH in plants (N.benthamiana, BY2 cells)

Depositor

Karen Sarkisyan

Article

Palkina et al Sci Adv. 2024 Mar 8;10(10):eadk1992. doi

Insert

nnLuz, nnH3H, nnCPH

Expression

Plant

Available Since

Oct. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pET20b-mPTER-H230F

Plasmid

#222195

Purpose

Bacterial expression of mouse PTER-H230F protein

Depositor

Jonathan Long

Article

Wei et al bioRxiv [Preprint]. 2024 Mar 22:2024.03.

Insert

PTER (Pter Mouse)

Tags

6xHis and Strep

Expression

Bacterial

Mutation

H230F

Available Since

Oct. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pET20b-mPTER-H26F

Plasmid

#222192

Purpose

Bacterial expression of mouse PTER-H26F protein

Depositor

Jonathan Long

Article

Wei et al bioRxiv [Preprint]. 2024 Mar 22:2024.03.

Insert

PTER (Pter Mouse)

Tags

6xHis and Strep

Expression

Bacterial

Mutation

H26F

Available Since

Oct. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pX459_TELO2_gRNA

Plasmid

#224379

Purpose

Targets TELO2 for dTAG N terminal knock-in cell lines

Depositor

Junjie Chen

Article

Huang et al Mol Cell. 2023 Aug 3;83(15):2810-2828.e6

Insert

TELO2 (TELO2 Human)

Use

CRISPR

Expression

Bacterial and Mammalian

Available Since

Oct. 7, 2024

Availability

Academic Institutions and Nonprofits only

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pX459_GNB1L_gRNA

Plasmid

#224377

Purpose

Targets GNB1L for dTAG C terminal knock-in cell lines

Depositor

Junjie Chen

Article

Huang et al Mol Cell. 2023 Aug 3;83(15):2810-2828.e6

Insert

GNB1L (GNB1L Human)

Use

CRISPR

Expression

Bacterial and Mammalian

Available Since

Oct. 7, 2024

Availability

Academic Institutions and Nonprofits only

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pPPI4-Strep-SNAP-CTLA-4-GCN4-His6

Plasmid

#223582

Purpose

Protein purification of human CTLA4 with Twin-Strep, SNAP, GCN4 and 6xHis tags from mammalian cells

Depositor

Enfu Hui

Article

Zhao et al Immunity. 2019 Dec 17;51(6):1059-1073.e9

Insert

CTLA4 (CTLA4 Human)

Tags

GCN4, His6 and Twin-Strep, SNAP

Expression

Mammalian

Available Since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pPPI4-Strep-SNAP-CD80 (I92R)-His10

Plasmid

#223580

Purpose

Protein purification of human CD80 (I92R) with Twin-Strep, SNAP and 10xHis tags from mammalian cells

Depositor

Enfu Hui

Article

Zhao et al Immunity. 2019 Dec 17;51(6):1059-1073.e9

Insert

CD80 (CD80 Human)

Tags

His10 and Twin-Strep, SNAP

Expression

Mammalian

Mutation

I92R

Available Since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pPPI4-Strep-SNAP-CD80-His10

Plasmid

#223579

Purpose

Protein purification of human CD80 with Twin-Strep, SNAP and 10xHis tags from mammalian cells

Depositor

Enfu Hui

Article

Zhao et al Immunity. 2019 Dec 17;51(6):1059-1073.e9

Insert

CD80 (CD80 Human)

Tags

His10 and Twin-Strep, SNAP

Expression

Mammalian

Available Since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pET20b-mPTER-E169A

Plasmid

#222190

Purpose

Bacterial expression of mouse PTER-E169A protein

Depositor

Jonathan Long

Article

Wei et al bioRxiv [Preprint]. 2024 Mar 22:2024.03.

Insert

PTER (Pter Mouse)

Tags

6xHis and Strep

Expression

Bacterial

Mutation

E169A

Available Since

Aug. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pET20b-mPTER-D298A

Plasmid

#222191

Purpose

Bacterial expression of mouse PTER-D298A protein

Depositor

Jonathan Long

Article

Wei et al bioRxiv [Preprint]. 2024 Mar 22:2024.03.

Insert

PTER (Pter Mouse)

Tags

6xHis and Strep

Expression

Bacterial

Mutation

D298A

Available Since

Aug. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pET20b-mPTER-H28F

Plasmid

#222193

Purpose

Bacterial expression of mouse PTER-H28F protein

Depositor

Jonathan Long

Article

Wei et al bioRxiv [Preprint]. 2024 Mar 22:2024.03.

Insert

PTER (Pter Mouse)

Tags

6xHis and Strep

Expression

Bacterial

Mutation

H28F

Available Since

Aug. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pET20b-mPTER-H201F

Plasmid

#222194

Purpose

Bacterial expression of mouse PTER-H201F protein

Depositor

Jonathan Long

Article

Wei et al bioRxiv [Preprint]. 2024 Mar 22:2024.03.

Insert

PTER (Pter Mouse)

Tags

6xHis and Strep

Expression

Bacterial

Mutation

H201A

Available Since

Aug. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pET20b-mPTER-R233A

Plasmid

#222197

Purpose

Bacterial expression of mouse PTER-R233A protein

Depositor

Jonathan Long

Article

Wei et al bioRxiv [Preprint]. 2024 Mar 22:2024.03.

Insert

PTER (Pter Mouse)

Tags

6xHis and Strep

Expression

Bacterial

Mutation

R233A

Available Since

Aug. 21, 2024

Availability

Academic Institutions and Nonprofits only

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Showing: 4241 - 4260 of 6978 results

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