We narrowed to 26,145 results for: Sis

1. pPHAGE_DLD-S456A-C-TAP

   Plasmid

   #83479

   Purpose

   Mammalian expression of the proteolysis-deficient mutant DLD-S456A.

   Depositor

   Mohan Babu

   Article

   Malty et al Cell Syst. 2017 Nov 7. pii: S2405-4712(1

   Insert

   DLD (DLD Human)
   

   Use

   Lentiviral

   Tags

   TAP

   Expression

   Mammalian

   Mutation

   S456A

   Promoter

   CMV

   Available since

   Nov. 10, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pGGG-Sr43

   Plasmid

   #186974

   Purpose

   Sr43 is a wheat stem rust resistance gene transferred from tall wheat grass (Thinopyrum ponticum) into bread wheat (Triticum aestivum).

   Depositor

   Brande Wulff

   Article

   Yu et al Nat Genet. 2023 Jun;55(6):921-926. doi:

   Insert

   Sr43
   

   Use

   Tags

   None

   Expression

   Bacterial and Plant

   Mutation

   None

   Promoter

   Natice

   Available since

   April 4, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pPHAGE-CR-PINK1-C-TAP

   Plasmid

   #128510

   Purpose

   Lentiviral constitutive expression of CRISPR-resistant PINK1 with c-terminal FLAG and HA tag.

   Depositor

   Mohan Babu

   Article

   Moutaoufik et al iScience. 2019 Sep 4;19:1114-1132. doi:

   Insert

   PTEN induced kinase 1 (PINK1 Human)
   

   Use

   Lentiviral

   Tags

   FLAG and HA tags

   Expression

   Mammalian

   Mutation

   Synonymous silent mutation in Q5 to render sgRNA …

   Promoter

   CMV

   Available since

   Sept. 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. Lenti-L227R-tevopreQ1-epegRNA+13C>A_EF1a-puroR (PBS 10 - RTT 19)

   Plasmid

   #207357

   Purpose

   Lentiviral transfer plasmid encoding hU6-driven expression of a L227R-CFTR correcting prime editing guide (epegRNA) and EF1a-driven puromycin resistance gene.

   Depositor

   Marianne Carlon

   Article

   Bulcaen et al Cell Rep Med. 2024 May 21;5(5):101544. d

   Insert

   L227R-CFTR + 13 C>A tevopreq1-prime editing guide RNA (epegRNA)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pENTR1A_mmFbxw7(res) (closed)

   Plasmid

   #160105

   Purpose

   Expresses murine Fbxw7 alpha with a T199 silent mutation in mammalian cells. The T199 silent mutation confers resistance to the 5'-TGAACATGGTACAAGGCCAG-3' sgRNA

   Depositor

   Rizwan Haq

   Article

   Gstalder et al Cancer Discov. 2020 May 5. pii: 2159-829

   Insert

   Fbxw7alpha (Fbxw7 Mouse)
   

   Use

   Gateway entry vector

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pDONR223-ALS2CR7

   Plasmid

   #23632

   Depositor

   William Hahn, David Root

   Article

   Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

   Insert

   ALS2CR7 (CDK15 Human)
   

   Use

   Gateway donor vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 30, 2010

   Availability

   Academic Institutions and Nonprofits only

7. pDONR223-LOC389599

   Plasmid

   #23361

   Depositor

   William Hahn, David Root

   Article

   Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

   Insert

   LOC389599 (STRADBP1 Human)
   

   Use

   Gateway donor vector

   Tags

   Expression

   Mutation

   C102T, several MMs

   Promoter

   Available since

   July 30, 2010

   Availability

   Academic Institutions and Nonprofits only

8. pCDH-3_Flag-YTHDF2-RNA-MUT (nonsense mutation of sgYTHDF2-1)

   Plasmid

   #232948

   Purpose

   Expresses the RNA-binding-deficient mutant of YTHDF2 with synonymous mutations for resistance against sgYTHDF2-1-mediated knockdown

   Depositor

   Jianjun Chen

   Article

   Chen et al Cell. 2024 Dec 12:S0092-8674(24)01324-2.

   Insert

   YTHDF2-RNA-MUT (YTHDF2 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   RNA binding mutation (K416A+R527A), synonymous mu…

   Promoter

   Available since

   May 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. Lenti-N1303K-tevopreQ1-epegRNA+13C>G_EF1a-puroR (PBS 14 - RTT 18)

   Plasmid

   #207356

   Purpose

   Lentiviral transfer plasmid encoding hU6-driven expression of a N1303K-CFTR correcting prime editing guide (epegRNA) and EF1a-driven puromycin resistance gene.

   Depositor

   Marianne Carlon

   Article

   Bulcaen et al Cell Rep Med. 2024 May 21;5(5):101544. d

   Insert

   N1303K-CFTR + 13 C>G tevopreq1-prime editing guide RNA (epegRNA)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pLX304_zeo_mmFbxw7(res)

    Plasmid

    #160106

    Purpose

    Expresses murine Fbxw7 alpha with a T199 silent mutation in mammalian cells. The T199 silent mutation confers resistance to the 5'-TGAACATGGTACAAGGCCAG-3' sgRNA

    Depositor

    Rizwan Haq

    Article

    Gstalder et al Cancer Discov. 2020 May 5. pii: 2159-829

    Insert

    Fbxw7alpha (Fbxw7 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pLV-hCdt1(1-100)ΔCy-mCherry-P2A-mVenus-hGeminin(1-110)-IRES-Blast

    Plasmid

    #193139

    Purpose

    Expresses bicistronic P2A construct of C-CRL4Cdt2 reporter(hCdt1(1-100)ΔCy-mCherry-) and APC/C reporter (hGeminin(1-110)) with blasticidin resistance

    Depositor

    Tobias Meyer

    Article

    Ratnayeke et al Mol Cell. 2023 Jan 5;83(1):26-42.e13. do

    Insert

    hCdt1(1-100)ΔCy-mCherry-P2A-mVenus-hGeminin(1-110) (CDT1, GMNN Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    human Cdt1 amino acids 1-100 w/ ΔCy mutation (aa6…

    Promoter

    EF1a

    Available since

    Jan. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pDONR223-NEK2

    Plasmid

    #23658

    Depositor

    William Hahn, David Root

    Article

    Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

    Insert

    NEK2 (NEK2 Human)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    Insert encodes truncated protein relative to NP_0…

    Promoter

    Available since

    July 30, 2010

    Availability

    Academic Institutions and Nonprofits only

13. pcDNA-v-Rel

    Plasmid

    #27256

    Depositor

    Thomas Gilmore

    Insert

    v-Rel
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 1, 2011

    Availability

    Academic Institutions and Nonprofits only

14. Nemato

    Plasmid

    #24006

    Depositor

    Lawrence Cohen

    Insert

    VSFP
    

    Use

    Tags

    Cerulean and Citruline

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 2, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pcDNA-FLAG-Nv-IKK

    Plasmid

    #27262

    Depositor

    Thomas Gilmore

    Insert

    Nv-IKK
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 16, 2011

    Availability

    Academic Institutions and Nonprofits only

16. pcDNA-FLAG-Nv-IkB

    Plasmid

    #27261

    Depositor

    Thomas Gilmore

    Insert

    Nv-IkB
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    P88Q

    Promoter

    Available since

    Feb. 13, 2012

    Availability

    Academic Institutions and Nonprofits only

17. pcDNA-FLAG-Nv-Bcl-3

    Plasmid

    #27263

    Depositor

    Thomas Gilmore

    Insert

    Nv-Bcl-3
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 1, 2011

    Availability

    Academic Institutions and Nonprofits only

18. pEMB52 / Anemone_LRRK2_1_334

    Plasmid

    #40550

    Depositor

    Michael J Fox Foundation MJFF

    Insert

    LRRK2
    

    Use

    Baculovirus

    Tags

    6xHis

    Expression

    Mutation

    WT

    Promoter

    polH

    Available since

    Oct. 1, 2012

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

19. pLAM12

    Plasmid

    #26908

    Depositor

    Graham Hatfull

    Article

    van Kessel et al Nat Methods. 2007 Feb . 4(2):147-52.

    Insert

    Acetamidase Promoter
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 3, 2011

    Availability

    Academic Institutions and Nonprofits only

20. pLCR0

    Plasmid

    #12137

    Depositor

    James Sherley

    Article

    Sherley et al J Biol Chem. 1991 Dec 25. 266(36):24815-

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 21, 2006

    Availability

    Academic Institutions and Nonprofits only