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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 5,159 results for: puc

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Showing: 4261 - 4280 of 5159 results

  3. pAS132

    Plasmid
    #211503
    Purpose
    AAV genome to express mCherry under control of EF1a. Use for tri-color experiments to define injection conditions for AAV-Perturb-seq
    Depositor
    Randall Platt
    Article
    Santinha et al Nature. 2023 Oct;622(7982):367-375. doi:
    Insert
    mCherry
    Use
    AAV, CRISPR, Cre/Lox, and Mouse Targeting
    Expression
    Mammalian
    Available Since
    Dec. 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    211503-plasmid-map-sequence-id-417791

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  5. qTAG-AAVS1-Ef1a-Puro-mScarlet

    Plasmid
    #227274
    Purpose
    AAVS1 targeting donor for the insertion of Puro and a strong EF1a promoter expressing mScarlet. To be co-transfected with sgRNA plasmid px330-AAVS1 (Addgene #227272)
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    AAVS1 Homology Arms flanking a 2A-Puro-EF1a-mScarlet cassette (AAVS1 Synthetic)
    Use
    CRISPR; Donor template
    Expression
    Mammalian
    Promoter
    Promoterless
    Available Since
    Nov. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    227274-plasmid-map-sequence-id-452035

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  6. AICSDP-43: GJA1-mEGFP

    Plasmid
    #109120
    Purpose
    Homology arms and linker-mEGFP sequence for C-terminus tagging of human GJA1
    Depositor
    Allen Institute for Cell Science
    Insert
    GJA1 Homology Arms with linker-mEGFP (GJA1 Human)
    Use
    CRISPR; Donor template
    Tags
    linker-mEGFP
    Expression
    Mammalian
    Mutation
    homology arms contain point mutations to disrupt …
    Available Since
    May 4, 2018
    Availability
    Industry, Academic Institutions, and Nonprofits
    109120-plasmid-map-sequence-id-209766

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  11. pEasyG3_mic

    Plasmid
    #184920
    Purpose
    Template to generate via PCR two gRNAs for expression in S. cerevisiae. The PCR product from pEasyG3_mic recombines in vivo with a PCR product from pEasyG3_zeo/nat/hph.
    Depositor
    Jeferson Gross
    Article
    Jacobus et al ACS Synth Biol. 2022 Nov 18;11(11):3886-
    Insert
    gRNA scaffold
    Use
    CRISPR
    Expression
    Bacterial
    Available Since
    Oct. 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    184920-plasmid-map-sequence-id-368413

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  14. AICSDP-29: LMNB1-mTagRFP-T

    Plasmid
    #114403
    Purpose
    Homology arms and mTagRFPT-linker sequence for N-terminus tagging of human LMNB1
    Depositor
    Allen Institute for Cell Science
    Insert
    LMNB1 Homology Arms with mTagRFPT-linker (LMNB1 Human)
    Use
    CRISPR; Donor template
    Tags
    mTagRFPT-linker
    Expression
    Mammalian
    Mutation
    homology arms contain point mutations to disrupt …
    Available Since
    Sept. 4, 2018
    Availability
    Industry, Academic Institutions, and Nonprofits
    114403-plasmid-map-sequence-id-220813

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  15. AICSDP-25:MAP1LC3B-mEGFP

    Plasmid
    #101783
    Purpose
    Homology arms and mEGFP-linker sequence for N-terminus tagging of human MAP1LC3B
    Depositor
    Allen Institute for Cell Science
    Insert
    MAP1LC3B Homology Arms with mEGFP-linker (MAP1LC3B Human)
    Use
    CRISPR; Donor template
    Tags
    mEGFP-linker
    Expression
    Mammalian
    Mutation
    homology arms contain point mutations to disrupt …
    Available Since
    Oct. 31, 2017
    Availability
    Industry, Academic Institutions, and Nonprofits
    101783-plasmid-map-sequence-id-197671

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  16. AICSDP-64: FUS-mEGFP

    Plasmid
    #114410
    Purpose
    Homology arms and linker-mEGFP sequence for C-terminus tagging of human FUS
    Depositor
    Allen Institute for Cell Science
    Insert
    FUS Homology Arms with linker-mEGFP (FUS Human)
    Use
    CRISPR; Donor template
    Tags
    linker-mEGFP
    Expression
    Mammalian
    Mutation
    homology arms contain point mutations to disrupt …
    Available Since
    Sept. 5, 2018
    Availability
    Industry, Academic Institutions, and Nonprofits
    114410-plasmid-map-sequence-id-220875

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  17. ATP1A1_G8_Dual_sgRNA

    Plasmid
    #178104
    Purpose
    Coselection for PE3 in human cells. Vector for tandem expression of ATP1A1 G8 sgRNA with a user-specified PE3 nick sgRNA from two independent U6 promoters. Cloning of oligos using BbsI sites.
    Depositor
    Yannick Doyon
    Article
    Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:
    Insert
    ATP1A1 G8 sgRNA + user-specified sgRNA
    Use
    CRISPR; Prime editing
    Expression
    Mammalian
    Promoter
    Tandem U6 promoters
    Available Since
    Dec. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    178104-plasmid-map-sequence-id-350193

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Showing: 4261 - 4280 of 5159 results