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pUBC-2X-HaloTag

Plasmid

#260113

Purpose

mammalian cell expression of tandem HaloTag

Depositor

Kai Zhang

Publication

Camp et al iScience. 2025 Oct 23;28(11):113848. doi

Insert

2X-HaloTag

Expression

Mammalian

Mutation

wild-type

Promoter

UBC

Available since

Aug. 24, 2026

Availability

Academic Institutions and Nonprofits only

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pUBC-1X-HaloTag

Plasmid

#260112

Purpose

mammalian cell expression of HaloTag

Depositor

Kai Zhang

Publication

Camp et al iScience. 2025 Oct 23;28(11):113848. doi

Insert

HaloTag

Expression

Mammalian

Mutation

wild-type

Promoter

UBC

Available since

Aug. 24, 2026

Availability

Academic Institutions and Nonprofits only

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pmTFP1-CAAX

Plasmid

#244037

Purpose

anchoring mTFP1 to the plasma membrane

Depositor

Markus Affolter

Publication

Vigano et al Biol Open. 2018 Oct 29;7(11). pii: bio.0

Insert

mTFP1

Tags

CAAX

Expression

Mammalian

Available since

Dec. 22, 2025

Availability

Academic Institutions and Nonprofits only

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pMITO-mTFP1

Plasmid

#244030

Purpose

anchoring mTFP1 to the mitochondrial membrane

Depositor

Markus Affolter

Publication

Vigano et al Biol Open. 2018 Oct 29;7(11). pii: bio.0

Insert

CISD1-mTFP1

Tags

sunTagV4

Expression

Mammalian

Available since

Nov. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3-mTFP1-Rab5c

Plasmid

#244031

Purpose

fusion of Rab5c to mTFP1

Depositor

Markus Affolter

Publication

Vigano et al Biol Open. 2018 Oct 29;7(11). pii: bio.0

Insert

mTFP1-Rab5c

Expression

Mammalian

Available since

Oct. 28, 2025

Availability

Academic Institutions and Nonprofits only

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pBBR-sfGFP N149TAG-MjtRNA

Plasmid

#242001

Purpose

Expresses MjtRNA and contains sfGFP gene with amber codon at 149 position.

Depositor

Wei Niu

Publication

He et al ACS Synth Biol. 2022 Nov 18;11(11):3724-

Insert

sfGFPN149TAG

Expression

Bacterial

Available since

Sept. 23, 2025

Availability

Academic Institutions and Nonprofits only

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pFREDusk-KanR-MCS

Plasmid

#229735

Purpose

For far-red light induced bacterial expression of gene of interest

Depositor

Heikki Takala

Publication

Bohm et al ACS Synth Biol. 2025 Jul 11. doi: 10.102

Type

Empty backbone

Expression

Bacterial

Available since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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pFREDusk-StrR-MCS

Plasmid

#229736

Purpose

For far-red light-induced bacterial expression of gene of interest (StrR)

Depositor

Heikki Takala

Publication

Bohm et al ACS Synth Biol. 2025 Jul 11. doi: 10.102

Type

Empty backbone

Expression

Bacterial

Available since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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pFREDusk-AmpR-MCS

Plasmid

#229737

Purpose

For far-red light-induced bacterial expression of gene of interest (AmpR)

Depositor

Heikki Takala

Publication

Bohm et al ACS Synth Biol. 2025 Jul 11. doi: 10.102

Type

Empty backbone

Expression

Bacterial

Available since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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mScarlet-I-Myo10 ΔF

Plasmid

#145139

Purpose

Expresses mScarlet-I tagged MYO10 construct with a MyTH/FERM domain deletion

Depositor

Johanna Ivaska

Publication

Miihkinen et al Cell Rep. 2021 Sep 14;36(11):109716. doi

Insert

MYO10 ΔF (MYO10 Human)

Expression

Mammalian

Available since

July 2, 2025

Availability

Academic Institutions and Nonprofits only

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EGFP-FERM (Myo10)

Plasmid

#145140

Purpose

Expresses EGFP-tagged MyTH/FERM domains of MYO10.

Depositor

Johanna Ivaska

Publication

Miihkinen et al Cell Rep. 2021 Sep 14;36(11):109716. doi

Insert

MyTH/FERM domains of MYO10 (MYO10 Human)

Expression

Mammalian

Available since

July 2, 2025

Availability

Academic Institutions and Nonprofits only

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EGFP-MYO10 TF

Plasmid

#145141

Purpose

Expresses EGFP-tagged MYO10 with MyTH/FERM swapped to Talin1 FERM domain.

Depositor

Johanna Ivaska

Publication

Miihkinen et al Cell Rep. 2021 Sep 14;36(11):109716. doi

Insert

MYO10 TF (MYO10 Human)

Expression

Mammalian

Available since

July 2, 2025

Availability

Academic Institutions and Nonprofits only

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pENTR2b ITGB1-tail-mTurquoise2

Plasmid

#145142

Purpose

Shuttle vector with mTurquoise2-tagged ITGB1 cytoplasmic tail as an insert

Depositor

Johanna Ivaska

Publication

Miihkinen et al Cell Rep. 2021 Sep 14;36(11):109716. doi

Insert

ITGB1-tail-mTurquoise2 (ITGB1 Human)

Use

Gateway Cloning

Available since

July 2, 2025

Availability

Academic Institutions and Nonprofits only

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pENTR2b ITGA2-tail-YPet

Plasmid

#145143

Purpose

Shuttle vector with YPet-tagged ITGA2 wild-type cytoplasmic tail as an insert

Depositor

Johanna Ivaska

Publication

Miihkinen et al Cell Rep. 2021 Sep 14;36(11):109716. doi

Insert

ITGA2-tail-YPet (ITGA2 Human)

Use

Gateway Cloning

Available since

July 2, 2025

Availability

Academic Institutions and Nonprofits only

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EGFP-MYO10 ΔF

Plasmid

#145816

Purpose

Expresses EGFP-tagged MYO10 with MyTH/FERM deletion in mammalian cells

Depositor

Johanna Ivaska

Publication

Miihkinen et al Cell Rep. 2021 Sep 14;36(11):109716. doi

Insert

Myo10 dFERM (MYO10 Human)

Tags

EGFP

Expression

Mammalian

Available since

July 2, 2025

Availability

Academic Institutions and Nonprofits only

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pBad_UlaABC

Plasmid

#216328

Purpose

Arabinose-inducible PBad promoter driving expression of ulaA, ulaB, and ulaC

Depositor

Mark Styczynski

Publication

Piorino et al ACS Synth Biol. 2022 Nov 18;11(11):3592-

Insert

UlaABC

Expression

Bacterial

Available since

Dec. 12, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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TurboID-MEK1

Plasmid

#226169

Purpose

Used to transiently express a TurboID fusion of the protein kinase MEK1.

Depositor

Dustin Maly

Publication

Simon et al Nat Methods. 2024 Nov;21(11):2094-2106.

Insert

Myc-TurboID-MEK1 (MAP2K1 Human)

Tags

Myc-TurboID

Expression

Mammalian

Available since

Nov. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pET28-PKP3(305-797)

Plasmid

#202599

Purpose

Expresses ARM domain of plakophilin3

Depositor

Tina Izard

Publication

Gupta et al Int J Mol Sci. 2023 May 29;24(11):9458.

Insert

plakophilin3 (PKP3 Human)

Tags

His8 tag with precision protease cleavage site

Expression

Bacterial

Mutation

Deleted amino acids 1 to 304

Available since

Nov. 7, 2024

Availability

Academic Institutions and Nonprofits only

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pNHA8

Plasmid

#223650

Purpose

BccP protein purification

Depositor

François-Xavier Campbell-Valois

Publication

Haidar-Ahmad et al mSphere. 2024 Nov 21;9(11):e0055324. doi

Insert

BccP

Tags

MBP

Expression

Bacterial

Promoter

LacI

Available since

Oct. 28, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pNHA10

Plasmid

#223554

Purpose

TurboID expression

Depositor

François-Xavier Campbell-Valois

Publication

Haidar-Ahmad et al mSphere. 2024 Nov 21;9(11):e0055324. doi

Insert

TurboID

Tags

Myc

Expression

Bacterial

Promoter

rpsM

Available since

Oct. 28, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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