Addgene

We narrowed to 11,396 results for: ARIA;

Showing: 4281 - 4300 of 11396 results

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pMCSG7-10xHis-ecRNHI*

Plasmid

#219523

Purpose

bacterial expression of cysteine-free variant of the E. coli ribonuclease HI gene

Depositor

Arthur Palmer

Article

Bhattacharya et al J Biomol NMR. 2024 Jun 10. doi: 10.1007/

Insert

cysteine-free variant of the E. coli ribonuclease HI gene

Use

Tags

N terminal 10xHis Tag

Expression

Bacterial

Mutation

Cysteine free variant

Promoter

Available since

June 3, 2024

Availability

Academic Institutions and Nonprofits only

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Pm_P41-Cd4-bio-His

Plasmid

#126824

Purpose

Expression of recombinant P. malariae protein in mammalian cells

Depositor

Gavin Wright

Article

Muller-Sienerth et al Malar J. 2020 Jan 17;19(1):31. doi: 10.1

Insert

P41 (P41 Plasmodium malariae)

Use

Tags

ratCd4(d3+4)-bio-6His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 31, 2022

Availability

Academic Institutions and Nonprofits only

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Pm_MSP3.5-Cd4-bio-His

Plasmid

#126825

Purpose

Expression of recombinant P. malariae protein in mammalian cells

Depositor

Gavin Wright

Article

Muller-Sienerth et al Malar J. 2020 Jan 17;19(1):31. doi: 10.1

Insert

MSP3.5 (PmUG01_06022600 Plasmodium malariae)

Use

Tags

ratCd4(d3+4)-bio-6His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 31, 2022

Availability

Academic Institutions and Nonprofits only

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Pm_MSP3.10-Cd4-bio-His

Plasmid

#126826

Purpose

Expression of recombinant P. malariae protein in mammalian cells

Depositor

Gavin Wright

Article

Muller-Sienerth et al Malar J. 2020 Jan 17;19(1):31. doi: 10.1

Insert

MSP3.10 (PmUG01_06022000 Plasmodium malariae)

Use

Tags

ratCd4(d3+4)-bio-6His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 31, 2022

Availability

Academic Institutions and Nonprofits only

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Pm_MSP7.1-Cd4-bio-His

Plasmid

#126827

Purpose

Expression of recombinant P. malariae protein in mammalian cells

Depositor

Gavin Wright

Article

Muller-Sienerth et al Malar J. 2020 Jan 17;19(1):31. doi: 10.1

Insert

MSP7.1 (PmUG01_12029500 Plasmodium malariae)

Use

Tags

ratCd4(d3+4)-bio-6His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 31, 2022

Availability

Academic Institutions and Nonprofits only

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Pm_MSP7.4-Cd4-bio-His

Plasmid

#126828

Purpose

Expression of recombinant P. malariae protein in mammalian cells

Depositor

Gavin Wright

Article

Muller-Sienerth et al Malar J. 2020 Jan 17;19(1):31. doi: 10.1

Insert

MSP7.4 (PmUG01_12029900 Plasmodium malariae)

Use

Tags

ratCd4(d3+4)-bio-6His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 31, 2022

Availability

Academic Institutions and Nonprofits only

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Pm_GAMA-Cd4-bio-His

Plasmid

#126818

Purpose

Expression of recombinant P. malariae protein in mammalian cells

Depositor

Gavin Wright

Article

Muller-Sienerth et al Malar J. 2020 Jan 17;19(1):31. doi: 10.1

Insert

GAMA (GAMA Plasmodium malariae)

Use

Tags

ratCd4(d3+4)-bio-6His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 31, 2022

Availability

Academic Institutions and Nonprofits only

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Pm_MSP10-Cd4-bio-His

Plasmid

#126819

Purpose

Expression of recombinant P. malariae protein in mammalian cells

Depositor

Gavin Wright

Article

Muller-Sienerth et al Malar J. 2020 Jan 17;19(1):31. doi: 10.1

Insert

MSP10 (MSP10 Plasmodium malariae)

Use

Tags

ratCd4(d3+4)-bio-6His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 31, 2022

Availability

Academic Institutions and Nonprofits only

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Pm_MSP4-Cd4-bio-His

Plasmid

#126820

Purpose

Expression of recombinant P. malariae protein in mammalian cells

Depositor

Gavin Wright

Article

Muller-Sienerth et al Malar J. 2020 Jan 17;19(1):31. doi: 10.1

Insert

MSP4 (PmUG01_04025600 Plasmodium malariae)

Use

Tags

ratCd4(d3+4)-bio-6His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 31, 2022

Availability

Academic Institutions and Nonprofits only

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Pm_MSP5-Cd4-bio-His

Plasmid

#126821

Purpose

Expression of recombinant P. malariae protein in mammalian cells

Depositor

Gavin Wright

Article

Muller-Sienerth et al Malar J. 2020 Jan 17;19(1):31. doi: 10.1

Insert

MSP5 (PmUG01_04025700 Plasmodium malariae)

Use

Tags

ratCd4(d3+4)-bio-6His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 31, 2022

Availability

Academic Institutions and Nonprofits only

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Pm_P38-Cd4-bio-His

Plasmid

#126823

Purpose

Expression of recombinant P. malariae protein in mammalian cells

Depositor

Gavin Wright

Article

Muller-Sienerth et al Malar J. 2020 Jan 17;19(1):31. doi: 10.1

Insert

P38 (P38 Plasmodium malariae)

Use

Tags

ratCd4(d3+4)-bio-6His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 31, 2022

Availability

Academic Institutions and Nonprofits only

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pRSETa-Amrose v2

Plasmid

#79538

Purpose

pRSETa (Invitrogen) plasmid expressing Amrose variant 2

Depositor

Randolph Caldwell

Article

Schoetz et al PLoS One. 2014 Sep 5;9(9):e107069. doi:

Insert

Amrose v2

Use

Tags

6xHis, T7 epitope, and Xpress tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

July 26, 2016

Availability

Academic Institutions and Nonprofits only

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pBG-ttLbUV2

Plasmid

#231386

Purpose

LbCas12a variant with D156R and E795L mutations and harboring same NLS sequence as PEmax but codon-optimized for dicot species; for Agrobacterium-mediated Arabidopsis transformation.

Depositor

Qi-Jun Chen

Article

Xin et al aBIOTECH. 2024 Mar 26;5(2):117-126. doi:

Insert

ttLbCas12a Ultra V2

Use

CRISPR

Tags

Expression

Plant

Mutation

D156R and E795L

Promoter

Available since

Feb. 21, 2025

Availability

Academic Institutions and Nonprofits only

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PET-HiFi SpCas9-NLS-6xHis

Plasmid

#207376

Purpose

Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

Depositor

Ervin Welker

Article

Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

Insert

HiFi SpCas9

Use

CRISPR

Tags

6xHis

Expression

Bacterial

Mutation

R691A

Promoter

T7

Available since

Sept. 25, 2023

Availability

Academic Institutions and Nonprofits only

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PET-eSpCas9-NLS-6xHis

Plasmid

#207379

Purpose

Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

Depositor

Ervin Welker

Article

Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

Insert

eSpCas9

Use

CRISPR

Tags

6xHis

Expression

Bacterial

Mutation

K848A, K1003A, R1060A

Promoter

T7

Available since

Sept. 25, 2023

Availability

Academic Institutions and Nonprofits only

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pBR322-TIMER

Plasmid

#103056

Purpose

medium copy plasmid for expression of a variant of the fluorescent timer protein suitable for bacteria under the control of the constitutive ybaJp promoter (works in E. coli and Salmonella enterica)

Depositor

Dirk Bumann

Article

Claudi et al Cell. 2014 Aug 14;158(4):722-733. doi: 1

Insert

TIMER-bac

Use

Tags

Expression

Bacterial

Mutation

mutations: S4T and S179T

Promoter

constitutive ybaJp promoter

Available since

Nov. 27, 2017

Availability

Academic Institutions and Nonprofits only

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C1-mCitrine-FKBP-EZRabd(t567D)

Plasmid

#155227

Purpose

Acute increase in MPA when used with FRB-FKBP dimerizaton system upon addition with Rapamycin

Depositor

Tobias Meyer

Article

Bisaria et al Science. 2020 Jun 12;368(6496):1205-1210

Insert

mCitrine fused to FKBP and the actin BD of Ezrin (T567D), aa552-586 for use with FRB and rapamycin induced dimerization system

Use

Tags

mCitrine

Expression

Mammalian

Mutation

Changed Thr 567 to Asp in original Ezrin

Promoter

Available since

Aug. 31, 2020

Availability

Academic Institutions and Nonprofits only

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SpCas9-NG

Plasmid

#138566

Purpose

Expresses human codon-optimized rationally engineered SpCas9 variant and blasticidin resistance: EFS promoter-SpCas9-NG-NLS-FLAG-P2A-BSD

Depositor

Hyongbum Kim

Article

Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133

Insert

SpCas9-NG

Use

CRISPR and Lentiviral

Tags

BSD, FLAG, and NLS

Expression

Mammalian

Mutation

L1111R, D1135V, G1218R, E1219F, A1322R, R1335V, T…

Promoter

EFS

Available since

June 9, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)- e18

Plasmid

#131670

Purpose

Expresses an engineered variant of RAD18 ("e18") that stimulates CRISPR mediated HDR.

Depositor

Alberto Ciccia

Article

Nambiar et al Nat Commun. 2019 Jul 30;10(1):3395. doi:

Insert

e18 (engineered RAD18 with SAP domain deleted) (RAD18 Human)

Use

Tags

FLAG and HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 26, 2019

Availability

Academic Institutions and Nonprofits only

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PET-WT SpCas9-NLS-6xHis

Plasmid

#207373

Purpose

Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

Depositor

Ervin Welker

Article

Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

Insert

WT SpCas9

Use

CRISPR

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

T7

Available since

Sept. 25, 2023

Availability

Academic Institutions and Nonprofits only

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