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pAAV.SynFLEX.(cyto).cpSFGFP.mIRFP670nano3

Plasmid

#244918

Purpose

cpSFGFP control with non-responsive mIRFP670nano3

Depositor

Tim Brown, HHMI-JRC Tool Translation Team

Article

Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

Insert

cpSFGFP.mIRFP670nano3

Use

AAV

Available Since

Oct. 8, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-Bach2_P2A_EGFP

Plasmid

#242200

Purpose

Expresses Bach2 with GFP in mammalian cells.

Depositor

Yusuke Kishi

Article

Bilgic et al Aging Cell. 2025 Sep 28:e70233. doi: 10.

Insert

Bach2 (Bach2 Mouse)

Use

AAV

Expression

Mammalian

Available Since

Oct. 1, 2025

Availability

Academic Institutions and Nonprofits only

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rTTA-gRNA-AAV

Plasmid

#213036

Purpose

This vector contains rTTA expressed under CMV promoter and gRNA expressed under U6 promoter

Depositor

Nadav Ahituv

Article

Nguyen et al Nat Biotechnol. 2025 Feb 4:10.1038/s4158

Insert

rTTA-T2A-mCherry

Use

AAV and CRISPR

Promoter

CMV

Available Since

Oct. 1, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-VTKS4-GCaMP6f

Plasmid

#209931

Purpose

Cre- and Flp-dependent transgene expression (CRE-ON / FLP-ON) of GCaMP6f in neurons under the control of the hSyn1 promoter. Based on construct from Pouchelon et al Cell Rep Methods. 2022.

Depositor

Jessica Cardin

Insert

GCaMP6f

Use

AAV, CRISPR, and Cre/Lox

Expression

Mammalian

Promoter

hSyn

Available Since

Oct. 1, 2025

Availability

Academic Institutions and Nonprofits only

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pAAVLINK-EF1alpha-5' BRD4

Plasmid

#236682

Purpose

5' AAVLINK plasmid for BRD4 expression

Depositor

Zhonghua Lu

Article

Lin et al Cell. 2026 Feb 5;189(3):969-986.e17. doi

Insert

BRD4 (BRD4 Human)

Use

AAV

Tags

HA

Promoter

EF1alpha

Available Since

Oct. 1, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV.hSynap.(ER).iGlucoSnFR2.HaloTag

Plasmid

#244076

Purpose

ER targeted expression of green glucose sensor with non-responsive HaloTag

Depositor

Tim Brown, HHMI-JRC Tool Translation Team

Article

Marvin et al Sci Adv. 2025 Nov 14;11(46):eadz3889. do

Insert

iGlucoSnFR2.HaloTag

Use

AAV

Available Since

Sept. 26, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV.CAGFLEX.(ER).iGlucoSnFR2.HaloTag

Plasmid

#244083

Purpose

ER targeted expression of green glucose sensor with non-responsive HaloTag

Depositor

Tim Brown, HHMI-JRC Tool Translation Team

Article

Marvin et al Sci Adv. 2025 Nov 14;11(46):eadz3889. do

Insert

iGlucoSnFR2.HaloTag

Use

AAV

Tags

IgG kappa leader and mIRFP670nano3

Available Since

Sept. 23, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV.CAGFLEX.(cyto).iGlucoSnFR2

Plasmid

#244078

Purpose

Cytosolic expression of green glucose sensor with non-responsive HaloTag

Depositor

Tim Brown, HHMI-JRC Tool Translation Team

Article

Marvin et al Sci Adv. 2025 Nov 14;11(46):eadz3889. do

Insert

iGlucoSnFR2

Use

AAV

Available Since

Sept. 23, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV.CAGFLEX.(mem).iGlucoSnFR2

Plasmid

#244081

Purpose

Plasma membrane targeted expression of green glucose sensor

Depositor

Tim Brown, HHMI-JRC Tool Translation Team

Article

Marvin et al Sci Adv. 2025 Nov 14;11(46):eadz3889. do

Insert

iGlucoSnFR2

Use

AAV

Tags

IgG kappa leader

Available Since

Sept. 23, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV.GFAP.(ER).iGlucoSnFR2.HaloTag

Plasmid

#244090

Purpose

ER targeted expression of green glucose sensor with non-responsive HaloTag

Depositor

Tim Brown, HHMI-JRC Tool Translation Team

Article

Marvin et al Sci Adv. 2025 Nov 14;11(46):eadz3889. do

Insert

iGlucoSnFR2.HaloTag

Use

AAV

Tags

IgG kappa leader and mIRFP670nano3

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-EFS-CasRx-pA

Plasmid

#192482

Purpose

To express CasRx

Depositor

Jonathan Ploski

Article

Back et al Mol Ther Methods Clin Dev. 2025 Aug 20;3

Insert

CasRx

Use

AAV

Mutation

No

Promoter

EFS

Available Since

Aug. 27, 2025

Availability

Academic Institutions and Nonprofits only

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hAAVS-RPL23a-SNAP

Plasmid

#242187

Purpose

Express RPL23a-SNAP in mammalian cells

Depositor

Joshua Mendell

Article

Lee et al Mol Cell. 2025 Apr 17;85(8):1543-1560.e1

Insert

RPL23a (RPL23A Human)

Tags

SNAP

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 22, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-puro_T11_EGFP-Responder

Plasmid

#230051

Purpose

It presents the T11 inducible promoter allowing the dox-dependent inducible activation of EGFP through the OPTi-OX platform

Depositor

Alessandro Bertero

Article

Guichardaz et al Open Res Eur. 2024 Dec 18;4:266. doi: 10

Insert

T11_EGFP

Expression

Mammalian

Available Since

Aug. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-puro_TRE3VG_mCherry-Responder

Plasmid

#230052

Purpose

It presents the TRE3VG inducible promoter allowing the dox-dependent inducible activation of mCherry through the OPTi-OX platform

Depositor

Alessandro Bertero

Article

Guichardaz et al Open Res Eur. 2024 Dec 18;4:266. doi: 10

Insert

mCherry

Expression

Mammalian

Available Since

Aug. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-puro_UCOE_TRE3VG_mCherry-Responder

Plasmid

#230053

Purpose

It presents the UCOE sequence and the TRE3VG inducible promoter allowing a more stable dox-dependent inducible activation of mCherry though the OPTi-OX platform across differentiation of hiPSCs

Depositor

Alessandro Bertero

Article

Guichardaz et al Open Res Eur. 2024 Dec 18;4:266. doi: 10

Insert

UCOE

Expression

Mammalian

Promoter

TRE3VG

Available Since

Aug. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-puro_TRE3VG_EGFP-Responder

Plasmid

#230050

Purpose

It presents the TRE3VG inducible promoter allowing the dox-dependent inducible activation of EGFP through the OPTi-OX platform

Depositor

Alessandro Bertero

Article

Guichardaz et al Open Res Eur. 2024 Dec 18;4:266. doi: 10

Insert

TRE3VG_EGFP

Expression

Mammalian

Promoter

GAAGACAATAGCAGGCATG

Available Since

Aug. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAAVLINK-EF1alpha-5' CHD2

Plasmid

#236658

Purpose

5' AAVLINK plasmid for CHD2 expression

Depositor

Zhonghua Lu

Article

Lin et al Cell. 2026 Feb 5;189(3):969-986.e17. doi

Insert

CHD2 (CHD2 Human)

Use

AAV

Tags

HA

Promoter

EF1alpha

Available Since

Aug. 12, 2025

Availability

Academic Institutions and Nonprofits only

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pAAVLINK-EF1alpha-5' SMARCA2

Plasmid

#236652

Purpose

5' AAVLINK plasmid for SMARCA2 expression

Depositor

Zhonghua Lu

Article

Lin et al Cell. 2026 Feb 5;189(3):969-986.e17. doi

Insert

SMARCA2 (SMARCA2 Human)

Use

AAV

Tags

HA

Promoter

EF1alpha

Available Since

Aug. 12, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-Tnnt2-HA-Luciferase

Plasmid

#227803

Purpose

cardiomyocytes-specific plasmid with Tnnt2 promotor, expressing the coding sequence of luciferase and a HA tag

Depositor

Yuxuan Guo

Article

Chen et al Fundam Res. 2025 Jun 30;5(6):2993-3000.

Insert

HA-Luciferase

Use

AAV

Expression

Mammalian

Promoter

Tnnt2

Available Since

Aug. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-eBRIC

Plasmid

#241801

Purpose

Bioluminescence indicator for Ca2+ imaging in neurons

Depositor

Huiwang Ai

Article

Tian et al ACS Sens. 2025 Jul 29. doi: 10.1021/acss

Inserts

eBRIC
eBRIC

Use

AAV

Available Since

July 28, 2025

Availability

Academic Institutions and Nonprofits only

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