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YSBE 559 : pNAT_6xHA

Plasmid

#92087

Purpose

Plasmid for tagging proteins in Cryptococcus neoformans; 6xHA tag, NAT selection

Depositor

Yong-Sun Bahn

Article

So et al J Microbiol Biotechnol. 2017 Feb 28;27(2

Insert

6xHA

Use

tagging proteins in cryptococcus neoforman

Available Since

July 10, 2017

Availability

Academic Institutions and Nonprofits only

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pFastBac FPH

Plasmid

#1925

Depositor

Robert Kingston

Article

Francis et al Mol Cell 2001 Sep;8(3):545-56.

Insert

polyhomeotic (ph-d, ph-p Fly)

Tags

Flag

Expression

Insect

Available Since

Aug. 3, 2006

Availability

Academic Institutions and Nonprofits only
pMaCTag-27

Plasmid

#120006

Purpose

Template for C-terminal PCR tagging of mammalian genes (without selection) with 1xHA

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

1xHA

Use

Pcr template

Tags

1xHA

Promoter

None

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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SNAP-EF(N)-5nmSAH-GFP(1-10)

Plasmid

#61659

Purpose

5nm SAH scaffold with EF Hand and split GFP attachment sites

Depositor

Kristen Verhey

Article

Norris et al J Cell Biol. 2014 Nov 10;207(3):393-406.

Insert

SNAP-EF(N)-5nmSAH-GFP(1-10)

Expression

Mammalian

Available Since

March 11, 2015

Availability

Academic Institutions and Nonprofits only

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mEGFP-30nmSAH-GFP(1-10)

Plasmid

#61677

Purpose

GFP-labeled SAH scaffold with split GFP attachment site

Depositor

Kristen Verhey

Article

Norris et al J Cell Biol. 2014 Nov 10;207(3):393-406.

Insert

mEGFP-30nmSAH-GFP(1-10)

Available Since

March 19, 2015

Availability

Academic Institutions and Nonprofits only

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7xNLS-pMJ915v2-sfGFP

Plasmid

#88922

Purpose

For expression of modified SpyCas9 in e.coli.

Depositor

Jennifer Doudna

Article

Staahl et al Nat Biotechnol. 2017 Feb 13. doi: 10.103

Inserts

Cas9
T7

Use

CRISPR

Expression

Bacterial

Available Since

May 4, 2017

Availability

Academic Institutions and Nonprofits only

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pET_His6-MBP-TEV-Cas2

Plasmid

#186275

Purpose

Expresses Thiomicrospira Cas2 in E. coli

Depositor

Jennifer Doudna

Article

Wang et al Nat Commun. 2021 May 6;12(1):2571. doi:

Insert

Thiomicropira Cas2

Tags

His6-MBP

Expression

Bacterial

Available Since

July 13, 2022

Availability

Academic Institutions and Nonprofits only

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HA Raptor mutant 7

Plasmid

#8475

Depositor

David Sabatini

Article

Kim et al Cell 2002 Jul 26;110(2):163-75.

Insert

Raptor (RPTOR Human)

Tags

ha

Expression

Mammalian

Mutation

738SLQN741-PAAA (S738P, L739A, Q740A, N741A)

Available Since

May 11, 2005

Availability

Academic Institutions and Nonprofits only
pAdTrackCMV-CDKN2A

Plasmid

#37105

Depositor

Joseph Nevins

Article

Angus et al Oncogene. 2012 Jan 16. doi: 10.1038/onc.

Insert

CDKN2A (CDKN2A Human)

Use

Adenoviral

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 5, 2012

Availability

Academic Institutions and Nonprofits only
pAcGP67A human LRP6 E3E4 (630–1244)

Plasmid

#132628

Purpose

expresses human LRP6 E3E4 (630–1244) in Hi5 insect cell

Depositor

Wenqing Xu

Article

Cheng et al Nat Struct Mol Biol. 2011 Oct 9;18(11):1

Insert

LRP6 (630–1244)

Expression

Insect

Available Since

Oct. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pAcGP67A human LRP6 E1E4 (20–1244)

Plasmid

#132629

Purpose

expresses human LRP6 E1E4 (20–1244) in Hi5 insect cell

Depositor

Wenqing Xu

Article

Cheng et al Nat Struct Mol Biol. 2011 Oct 9;18(11):1

Insert

LRP6 (20–1244)

Expression

Insect

Available Since

Oct. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pJZC572

Plasmid

#62318

Purpose

sgRNA with 1x com for yeast cells

Depositor

Wendell Lim, Stanley Qi

Article

Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

Insert

sgRNA + 1x com RNA binding module

Expression

Yeast

Promoter

SNR52

Available Since

March 18, 2015

Availability

Academic Institutions and Nonprofits only

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pEPYCeGM0001

Plasmid

#196141

Purpose

Gateway entry vector with Arabidopsis TGA1 CDS and c-terminal HiBiT tag

Depositor

Nicola Patron

Article

Cai et al ACS Synth Biol. 2023 Nov 17;12(11):3482-

Insert

AtTGA1_HiBit

Use

Gateway entry vector

Available Since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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pAcrF3

Plasmid

#89246

Purpose

Plasmid contains the gene for anti-CRISPR protein AcrF3 (gene product 35 from bacteriophage JBD5).

Depositor

Blake Wiedenheft

Article

Rollins et al Proc Natl Acad Sci U S A. 2017 Apr 24. p

Insert

AcrF3

Tags

6xHis-TEV

Available Since

April 28, 2017

Availability

Academic Institutions and Nonprofits only

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pGEX-2T Crkl SH3SH3

Plasmid

#36403

Depositor

Nora Heisterkamp

Article

de Jong et al J Biol Chem. 1995 Sep 15;270(37):21468-7

Insert

Crkl tandem SH3 domains (CRKL Human)

Tags

GST

Expression

Bacterial

Available Since

June 21, 2012

Availability

Academic Institutions and Nonprofits only
pET15b-At 14-3-3 epsilon

Plasmid

#44936

Depositor

Robert Ferl

Article

Sehnke et al Plant Physiol. 2006 Feb;140(2):647-60. E

Insert

14-3-3 epsilon (GRF10 Mustard Weed)

Tags

His tag

Expression

Bacterial

Promoter

T7

Available Since

May 14, 2013

Availability

Academic Institutions and Nonprofits only
pECFP-Sec23A

Plasmid

#66610

Purpose

CFP fusion of Sec23A

Depositor

David Stephens

Article

Stephens et al J Cell Sci. 2000 Jun;113 ( Pt 12):2177-8

Insert

Sec23A (SEC23A Human)

Tags

ECFP

Expression

Mammalian

Available Since

July 28, 2015

Availability

Academic Institutions and Nonprofits only
Sa-SchCas9

Plasmid

#192137

Purpose

Expresses Sa-SchCas9, and cloning backbone for sgRNA

Depositor

Yongming Wang

Article

Wang et al Adv Sci (Weinh). 2022 Feb;9(4):e2104789.

Insert

Sa-SchCas9

Use

AAV

Expression

Mammalian

Available Since

March 22, 2023

Availability

Academic Institutions and Nonprofits only

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AP568-3

Plasmid

#70048

Purpose

co-expression of Cas9 and crRNA 589 target sequence

Depositor

Geraldine Seydoux

Article

Paix et al Genetics. 2015 Sep;201(1):47-54. doi: 10

Insert

sgRNA for dpy‐10

Use

CRISPR

Expression

Worm

Available Since

Nov. 13, 2015

Availability

Academic Institutions and Nonprofits only

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pCMV-Flag-YAP-R89A

Plasmid

#33058

Depositor

Kunliang Guan

Article

Li et al Genes Dev. 2010 Feb 1;24(3):235-40.

Insert

YAP (YAP1 Human)

Tags

Flag

Expression

Mammalian

Mutation

R89A

Available Since

Feb. 10, 2012

Availability

Academic Institutions and Nonprofits only