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pCYP16

Plasmid

#212849

Purpose

Expresses the Actinomadura pelletieri LRD-modifying cytochrome P450 enzyme

Depositor

Christopher Voigt

Article

Lin et al Nature Catalysis. 6, 1016–1029 (2023). h

Insert

cytochrome P450

Expression

Bacterial

Promoter

PcymRC

Available Since

Feb. 22, 2024

Availability

Academic Institutions and Nonprofits only

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pCYP27

Plasmid

#212860

Purpose

Expresses the Kitasatospora sp. CB02891 LRD-modifying cytochrome P450 enzyme

Depositor

Christopher Voigt

Article

Lin et al Nature Catalysis. 6, 1016–1029 (2023). h

Insert

cytochrome P450

Expression

Bacterial

Promoter

PcymRC

Available Since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pCYP33

Plasmid

#212866

Purpose

Expresses the Streptomyces platensis LRD-modifying cytochrome P450 enzyme

Depositor

Christopher Voigt

Article

Lin et al Nature Catalysis. 6, 1016–1029 (2023). h

Insert

cytochrome P450

Expression

Bacterial

Promoter

PcymRC

Available Since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pCYP28

Plasmid

#212861

Purpose

Expresses the Micromonospora humi LRD-modifying cytochrome P450 enzyme

Depositor

Christopher Voigt

Article

Lin et al Nature Catalysis. 6, 1016–1029 (2023). h

Insert

cytochrome P450

Expression

Bacterial

Promoter

PcymRC

Available Since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pCYP52

Plasmid

#212885

Purpose

Expresses the Herbihabitans rhizosphaerae LRD-modifying cytochrome P450 enzyme

Depositor

Christopher Voigt

Article

Lin et al Nature Catalysis. 6, 1016–1029 (2023). h

Insert

cytochrome P450

Expression

Bacterial

Promoter

PcymRC

Available Since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pCYP39

Plasmid

#212872

Purpose

Expresses the Saccharothrix texasensis LRD-modifying cytochrome P450 enzyme

Depositor

Christopher Voigt

Article

Lin et al Nature Catalysis. 6, 1016–1029 (2023). h

Insert

cytochrome P450

Expression

Bacterial

Promoter

PcymRC

Available Since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pCYP25

Plasmid

#212858

Purpose

Expresses the Streptomyces sp. HB202 LRD-modifying cytochrome P450 enzyme

Depositor

Christopher Voigt

Article

Lin et al Nature Catalysis. 6, 1016–1029 (2023). h

Insert

cytochrome P450

Expression

Bacterial

Promoter

PcymRC

Available Since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pCYP04

Plasmid

#212837

Purpose

Expresses the Rhizobium grahamii LRD-modifying cytochrome P450 enzyme

Depositor

Christopher Voigt

Article

Lin et al Nature Catalysis. 6, 1016–1029 (2023). h

Insert

cytochrome P450

Expression

Bacterial

Promoter

PcymRC

Available Since

Feb. 7, 2024

Availability

Academic Institutions and Nonprofits only

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pCYP02

Plasmid

#212835

Purpose

Expresses the Sinorhizobium fredii LRD-modifying cytochrome P450 enzyme

Depositor

Christopher Voigt

Article

Lin et al Nature Catalysis. 6, 1016–1029 (2023). h

Insert

cytochrome P450

Expression

Bacterial

Promoter

PcymRC

Available Since

Feb. 7, 2024

Availability

Academic Institutions and Nonprofits only

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pCYP06

Plasmid

#212839

Purpose

Expresses the Rhizobium acidisoli LRD-modifying cytochrome P450 enzyme

Depositor

Christopher Voigt

Article

Lin et al Nature Catalysis. 6, 1016–1029 (2023). h

Insert

cytochrome P450

Expression

Bacterial

Promoter

PcymRC

Available Since

Jan. 24, 2024

Availability

Academic Institutions and Nonprofits only

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Lenti-(BB)-EF1a-KRAB-dCas9-P2A-EGFP

Plasmid

#118156

Purpose

Catalytically inactive Cas9 from S. pyogenes with P2A-EGFP under the EF1a core promoter, and cloning backbone for sgRNA. Contains BsmBI sites for insertion of spacer sequences.

Depositor

Jorge Ferrer

Insert

KRAB-dCas9-P2A-EGFP

Use

CRISPR and Lentiviral

Tags

HA

Expression

Mammalian

Promoter

EF1a core and U6

Available Since

April 1, 2019

Availability

Academic Institutions and Nonprofits only

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pMarC9-R6k

Plasmid

#89477

Purpose

transposon plasmid, compatible with Tn-Seq

Depositor

George Church

Article

Lee et al Nat Microbiol. 2019 Apr 8. pii: 10.1038/

Insert

pMarC9-R6k

Available Since

Oct. 20, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_crGE

Plasmid

#172933

Purpose

Crowding FRET sensor with mCerulean3 and mCitrine for mammalian cell expression. Linker comprises 2 helices and 3 GSG domains.

Depositor

Arnold Boersma

Article

Boersma et al Nat Methods. 2015 Mar;12(3):227-9, 1 p f

Insert

CrowdingSensorGE

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pDpy-7::oxCerulean-oxVenus::Lgg-1

Plasmid

#68042

Purpose

Lgg-1 tagged with oxCerulean-oxVenus (dFP, or double fluorescent protein), expressed in hypodermis, for monitoring autophagy

Depositor

Dana Miller

Article

Chapin et al Aging (Albany NY). 2015 Jun 30.

Insert

pDpy-7::oxCerulean-oxVenus::Lgg-1

Expression

Worm

Available Since

Sept. 10, 2015

Availability

Academic Institutions and Nonprofits only

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pMEV4

Plasmid

#110539

Purpose

The plasmid serves as a backbone for heterologous expression in the model archaeal organism Methanococcus maripaludis.

Depositor

William B. Whitman

Article

Lyu et al ACS Synth Biol. 2016 Jul 15;5(7):577-81.

Type

Empty backbone

Use

Synthetic Biology

Available Since

Aug. 28, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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pCYP01

Plasmid

#212834

Purpose

Expresses the Rhizobium aethiopicum LRD-modifying cytochrome P450 enzyme

Depositor

Christopher Voigt

Article

Lin et al Nature Catalysis. 6, 1016–1029 (2023). h

Insert

cytochrome P450

Expression

Bacterial

Promoter

PcymRC

Available Since

Jan. 24, 2024

Availability

Academic Institutions and Nonprofits only

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sZF2

Plasmid

#43833

Depositor

George Church

Article

Mali et al Nat Methods. 2013 Mar 17. doi: 10.1038/n

Insert

sZF2

Tags

HA Flag and Myc

Expression

Mammalian

Promoter

CMV

Available Since

March 11, 2013

Availability

Academic Institutions and Nonprofits only
sZF1

Plasmid

#43832

Depositor

George Church

Article

Mali et al Nat Methods. 2013 Mar 17. doi: 10.1038/n

Insert

sZF1

Tags

HA Flag and Myc

Expression

Mammalian

Promoter

CMV

Available Since

March 11, 2013

Availability

Academic Institutions and Nonprofits only
pRSETA_crGE

Plasmid

#172927

Purpose

Crowding FRET sensor with mCerulean3 and mCitrine for E. coli expression. Linker comprises 2 helices and 3 GSG domains.

Depositor

Arnold Boersma

Article

Boersma et al Nat Methods. 2015 Mar;12(3):227-9, 1 p f

Insert

CrowdingSensorGE

Tags

His

Expression

Bacterial

Promoter

T7

Available Since

Aug. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pJKR-H-benM

Plasmid

#73925

Purpose

Muconate biosensor. Expresses GFP proportional to muconate concentration within the cell.

Depositor

George Church

Article

Rogers et al Proc Natl Acad Sci U S A. 2016 Feb 8. pi

Insert

muconate biosensing apparatus

Available Since

Jan. 14, 2017

Availability

Academic Institutions and Nonprofits only

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