We narrowed to 11,396 results for: ARIA;

1. pBBR_Cas9_sp1

   Plasmid

   #154851

   Purpose

   Expresses SpCas9 and sgRNA that targets the upp gene in Rhodobacter sphaeroides genome

   Depositor

   John van der Oost

   Article

   Mougiakos et al Microb Cell Fact. 2019 Nov 25;18(1):204.

   Inserts

   Spcas9 gene
   sgRNA scaffold
   

   Use

   CRISPR

   Tags

   6xHis

   Expression

   Mutation

   Codon harmonized variant for expression in Rhodob…

   Promoter

   Available since

   Aug. 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTPH413

   Plasmid

   #185728

   Purpose

   Bacterial PAM profiling of adenine base editor variants

   Depositor

   David Liu

   Article

   Huang et al Nat Biotechnol. 2022 Sep 8. pii: 10.1038

   Inserts

   ecTadA(8e)-neNme2-C
   Nme2Cas9 sgRNA
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Nme2Cas9 P6S/D16A/E33G/K104T/D152A/F260L/A263T/A3…

   Promoter

   Available since

   Sept. 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. xCas9

   Plasmid

   #138565

   Purpose

   Expresses human codon-optimized expanded PAM SpCas9 variant and blasticidin resistance: EFS promoter-xCas9-NLS-FLAG-P2A-BSD

   Depositor

   Hyongbum Kim

   Article

   Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133

   Insert

   xCas9
   

   Use

   CRISPR and Lentiviral

   Tags

   BSD, FLAG, and NLS

   Expression

   Mammalian

   Mutation

   A262T, R324L, S409I, E480K, E543D, M694I, E1219V

   Promoter

   EFS

   Available since

   June 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. eGFP-KIF5A

   Plasmid

   #172201

   Purpose

   Expresses KIF5A tagged with eGFP in mammalian cells

   Depositor

   Juan Bonifacino

   Article

   Farias et al Cell Rep. 2015 Nov 10;13(6):1221-1232. d

   Insert

   KIF5A (Kif5a Mouse)
   

   Use

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET30b jGCaMP8f F366H

   Plasmid

   #204139

   Purpose

   Production of recombinant calcium sensor jGCaMP8f F366H high dynamic range variant in E.coli

   Depositor

   Katalin Torok

   Article

   Tran et al Front Cell Neurosci. 2023 May 18;17:1155

   Insert

   jGCaMP8f F366H
   

   Use

   Tags

   N-terminal His tag

   Expression

   Bacterial

   Mutation

   Phenylalanine 366 changed to histidine

   Promoter

   T7

   Available since

   Dec. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBabe puro RalA Q75L

   Plasmid

   #19719

   Depositor

   Channing Der

   Article

   Lim et al Curr Biol. 2006 Dec 19. 16(24):2385-94.

   Insert

   V-ral simian leukemia viral oncogene homolog A (RALA Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   GTPase deficient & constitutively active Gl…

   Promoter

   Available since

   Oct. 27, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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7. pVRC8400-SARS-CoV-2-S2P-BA.4/5-AVI

   Plasmid

   #192112

   Purpose

   Expresses SARS-CoV-2 S2P protein from the BA.4 and BA.5 variants with single chain Fc, HRV3C protease cleavage site, and AVI tag

   Depositor

   Peter Kwong

   Insert

   SARS-CoV-2-S2P-BA.4/5 (S )
   

   Use

   Tags

   AVI, HRV3C, and scFc

   Expression

   Mammalian

   Mutation

   T19I, L24S, P25-, P26-, A27-, H69-, V70-, G142D, …

   Promoter

   Available since

   Oct. 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBabe puro RalA S31N

   Plasmid

   #19718

   Depositor

   Channing Der

   Article

   Lim et al Curr Biol. 2006 Dec 19. 16(24):2385-94.

   Insert

   V-ral simian leukemia viral oncogene homolog A (RALA Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   S31N mutant for dominant neg function. NOTE: lo…

   Promoter

   Available since

   Oct. 27, 2008

   Availability

   Academic Institutions and Nonprofits only

9. C1-F-tractin(delta1-6)-mCitrine

   Plasmid

   #155219

   Purpose

   F-actin bidning mutant of F-tractin

   Depositor

   Tobias Meyer

   Article

   Bisaria et al Science. 2020 Jun 12;368(6496):1205-1210

   Insert

   F-tractin (aa 9-52 of rat ITPKA) fused to mCitrine (Itpka Rat)
   

   Use

   Tags

   mCitrine

   Expression

   Mammalian

   Mutation

   Deletion of first 6 AA from F-tractin (9-14 from …

   Promoter

   Available since

   Sept. 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSG5-ERRbeta-delta10

    Plasmid

    #52187

    Purpose

    Expresses human ERRbeta-delta10 splice variant in mammalian cells

    Depositor

    Rebecca Riggins

    Article

    Heckler et al Cell Cycle. 2014 Dec 12:0.

    Insert

    Estrogen-related receptor beta (ESRRB Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    wild type sequence; codon optimized to exclude Ec…

    Promoter

    SV40

    Available since

    April 24, 2014

    Availability

    Academic Institutions and Nonprofits only

11. pInducer21-3xFlag-S208C-MEFV

    Plasmid

    #167019

    Purpose

    Lentiviral plasmid with 3xFlag-pyrin (S208C/PAAND variant) under the control of a doxycycline-inducible promoter

    Depositor

    Thomas Henry

    Article

    Chirita et al Methods Mol Biol. 2022;2523:179-195. doi

    Insert

    MEFV (MEFV Human)
    

    Use

    Lentiviral

    Tags

    3xFlag

    Expression

    Mutation

    Serine 208 changed to Cysteine

    Promoter

    CMV Dox inducible

    Available since

    April 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. p3R112C/T180CBM3

    Plasmid

    #85090

    Purpose

    Expresses PCNA3 variant (R112C/T180C) fused to P450 BM3 variant (A74G/C773S/C810S) in E. coli

    Depositor

    Teruyuki Nagamune

    Article

    Tan et al Angew Chem Int Ed Engl. 2016 Oct 26. doi

    Insert

    The R112C/T180C variant of PCNA3 fused to P450 BM3
    

    Use

    Tags

    His6 tag

    Expression

    Bacterial

    Mutation

    R112C/T180C in PCNA3, A74G/C773S/C810S in P450BM3

    Promoter

    T7 promoter

    Available since

    Dec. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. AAV r3.45

    Plasmid

    #233684

    Purpose

    An evolved Adeno-associated viral variant, enhances gene delivery and gene targeting in neural stem cells

    Depositor

    David Schaffer

    Article

    Jang et al Mol Ther. 2011 Apr;19(4):667-75. doi: 10

    Insert

    Modified AAV2 Rep, Cap
    

    Use

    AAV

    Tags

    Expression

    Mutation

    insertion of LATQVGQKTA (where LA and A are linke…

    Promoter

    Available since

    April 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET30b jGCaMP8f L27A

    Plasmid

    #204138

    Purpose

    Production of recombinant calcium sensor jGCaMP8f L27A fast decay variant in E.coli

    Depositor

    Katalin Torok

    Article

    Tran et al Front Cell Neurosci. 2023 May 18;17:1155

    Insert

    jGCaMP8f L27A
    

    Use

    Tags

    N-terminal His tag

    Expression

    Bacterial

    Mutation

    Leucine 27 changed to Alanine

    Promoter

    T7

    Available since

    Dec. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. PET-HypaSpCas9-NLS-6xHis

    Plasmid

    #207384

    Purpose

    Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

    Insert

    HypaSpCas9
    

    Use

    CRISPR

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    N692A, M694A, Q695A, H698A

    Promoter

    T7

    Available since

    Oct. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. PET-evoSpCas9-NLS-6xHis

    Plasmid

    #207389

    Purpose

    Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

    Insert

    evoSpCas9
    

    Use

    CRISPR

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    M495V, Y515N, K526E, R661Q

    Promoter

    T7

    Available since

    Sept. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pNHT7::UMSBP::sNluc2_ORF1

    Plasmid

    #186765

    Purpose

    An mRNA production vector encoding planarian codon-optimized sNluc2 with UMSBP 5' and 3' UTRs beginning at the endogenous gene's start codon.

    Depositor

    Bo Wang

    Article

    Hall et al Cell Rep Methods. 2022 Sep 20;2(10):1002

    Insert

    Nanoluciferase
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    Oct. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pNHT7::ENO::sNluc2

    Plasmid

    #186770

    Purpose

    An mRNA production vector encoding planarian codon-optimized nanoluciferase (sNluc2) with enolase 5' and 3' UTRs

    Depositor

    Bo Wang

    Article

    Hall et al Cell Rep Methods. 2022 Sep 20;2(10):1002

    Insert

    Nanoluciferase
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    Oct. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pNHT7::RPL15::sNluc0

    Plasmid

    #186771

    Purpose

    An mRNA production vector encoding planarian codon-optimized nanoluciferase (sNluc0) with RPL15 5' and 3' UTRs

    Depositor

    Bo Wang

    Article

    Hall et al Cell Rep Methods. 2022 Sep 20;2(10):1002

    Insert

    Nanoluciferase
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    Oct. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pNHT7::YB1::sNluc1

    Plasmid

    #186754

    Purpose

    An mRNA production vector encoding planarian codon-optimized nanoluciferase (sNluc1, CAI = 0.925) with y-box binding protein 5' and 3' UTRs.

    Depositor

    Bo Wang

    Article

    Hall et al Cell Rep Methods. 2022 Sep 20;2(10):1002

    Insert

    Nanoluciferase
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    Oct. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge