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pHR_Gal4UAS_human_leucinezipperTRAIL _IRES_mCherry_PGK_tBFP

Plasmid

#85433

Purpose

Lentiviral vector for Gal4 inducible leucinezipper TRAIL with mCherry reporter and constitutive tBFP expression

Depositor

Wendell Lim

Article

Roybal et al Cell. 2016 Oct 6;167(2):419-432.e16. doi

Insert

leucine zipper human TRAIL secreted form

Use

Lentiviral

Expression

Mammalian

Available Since

Nov. 23, 2017

Availability

Academic Institutions and Nonprofits only
pSEZ-1

Plasmid

#51868

Purpose

Expression of cpYFP with a mitochondrial-targeting sequence (mt-cpYFP) in C. elegans

Depositor

Meng-Qiu Dong

Article

Shen et al Nature. 2014 Feb 12. doi: 10.1038/nature

Insert

psdhb-1::mtLS-cpYFP

Expression

Worm

Promoter

sdhb-1

Available Since

March 26, 2014

Availability

Academic Institutions and Nonprofits only

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pLenti_C_Myc_DDK_IRES_Puro_PRKRA

Plasmid

#106113

Purpose

Lentiviral expression of PRKRA

Depositor

Thomas Tuschl

Article

Meyer et al Mol Cell. 2018 Feb 15;69(4):622-635.e6.

Insert

PRKRA (PRKRA Human)

Use

Lentiviral

Tags

DDK (FLAG) and Myc

Available Since

Feb. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pHR_Gal4UAS_humanTRAIL _IRES_mCherry_PGK_tBFP

Plasmid

#85432

Purpose

Lentiviral vector for Gal4 inducible human full-length TRAIL with mCherry reporter and constitutive tBFP expression

Depositor

Wendell Lim

Article

Roybal et al Cell. 2016 Oct 6;167(2):419-432.e16. doi

Insert

human TRAIL

Use

Lentiviral

Expression

Mammalian

Available Since

Sept. 19, 2017

Availability

Academic Institutions and Nonprofits only
PdnaK GFP pZa

Plasmid

#170073

Purpose

GFP expression under the control of E. coli dnaK promoter

Depositor

Urartu Seker

Article

Saltepe et al ACS Synth Biol. 2019 Oct 18;8(10):2404-2

Insert

Green fluorescent protein

Use

Synthetic Biology

Expression

Bacterial

Promoter

PdnaK

Available Since

Sept. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-CASI-Cas9C-P2A-turboGFP

Plasmid

#80942

Purpose

Expresses Cas9C in mammalian cells; co-expresses turboGFP.

Depositor

George Church

Article

Chew et al Nat Methods. 2016 Sep 5. doi: 10.1038/nm

Insert

Cas9C

Use

AAV, CRISPR, and Synthetic Biology

Expression

Mammalian

Available Since

Oct. 3, 2016

Availability

Academic Institutions and Nonprofits only

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pCS2-epNTR

Plasmid

#62213

Purpose

epNTR (zebrafish optimized nitroreductase, T41L, N71S, F124W) in pCS2

Depositor

Harold Burgess

Article

Tabor et al J Neurophysiol. 2014 Aug 15;112(4):834-4

Insert

epNTR

Use

Mrna synthesis

Available Since

Feb. 5, 2015

Availability

Academic Institutions and Nonprofits only

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pEF.DEST51-mVenus

Plasmid

#154899

Purpose

Expresses a fluorescent protein, mVenus

Depositor

Johanna Ivaska

Article

Al-Akhrass et al Oncogene. 2021 Feb;40(7):1300-1317. doi:

Insert

mVenus

Expression

Mammalian

Promoter

EF-1α

Available Since

July 27, 2020

Availability

Academic Institutions and Nonprofits only

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lenti-CMV-TFAM-BioID2-IRES-Puro

Plasmid

#211465

Purpose

Lentivirus backbone expressing human TFAM fused with BioID2

Depositor

Agnel Sfeir

Article

Fu et al Mol Cell. 2023 Oct 8:S1097-2765(23)00753

Insert

TFAM-BioID2

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pLARRYv2-EGFP

Plasmid

#233208

Purpose

Cloning expressed barcodes at the EcoRV site for preparing lentiviral libaries for single-cell lineage tracing experiments, with EGFP marker

Depositor

Alejo Rodriguez-Fraticelli

Article

Singh et al Cell Stem Cell. 2025 Feb 19:S1934-5909(2

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available Since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pJJiaDEST-APEX2

Plasmid

#149517

Purpose

N-termial APEX2 tag. Gateway destination vector for mammanlian expression

Depositor

Vojo Deretic

Article

Jia et al Mol Cell. 2018 Apr 5;70(1):120-135.e8. d

Type

Empty backbone

Tags

FLAG-APEX2

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pHR_PGK_antiHer24D5-8_synNotch_Gal4VP64

Plasmid

#85425

Purpose

Lentiviral vector for expression of anti-Her2 scFv 24D5-8 Gal4VP64 synthetic Notch

Depositor

Wendell Lim

Article

Roybal et al Cell. 2016 Oct 6;167(2):419-432.e16. doi

Insert

anti-Her2_4D5-8_synNotch

Use

Lentiviral

Promoter

PGK

Available Since

July 31, 2018

Availability

Academic Institutions and Nonprofits only
pFRT_TO_FlagHA_PRKRA

Plasmid

#106111

Purpose

Inducible gene expression of FlagHA-PRKRA

Depositor

Thomas Tuschl

Article

Meyer et al Mol Cell. 2018 Feb 15;69(4):622-635.e6.

Insert

PRKRA (PRKRA Human)

Tags

Flag and HA

Expression

Mammalian

Available Since

Feb. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pDV05.1_pEF1a-iRFP720_frameshifted

Plasmid

#200408

Purpose

Expresses a mutant iRFP720 mRNA constitutively in mammalian cells. A frameshift deletion in the ORF (compared to pDV05.0) prevents the correct translation of the fluorescent protein

Depositor

James Collins

Article

Gayet et al Nat Commun. 2023 Mar 11;14(1):1339. doi:

Insert

iRFP720 (frameshift mutant)

Use

Synthetic Biology

Expression

Mammalian

Available Since

June 30, 2023

Availability

Academic Institutions and Nonprofits only

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pBLADE_ONLY_A

Plasmid

#168051

Purpose

Expresses the synthetic gene BLADE FP6 under the constitutive J23101** promoter.

Depositor

Barbara Di Ventura

Article

Romano et al Nat Chem Biol. 2021 Apr 26. pii: 10.1038

Insert

Blue light-inducible AraC dimers in Escherichia coli

Expression

Bacterial

Promoter

J23101**

Available Since

May 11, 2021

Availability

Academic Institutions and Nonprofits only

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pDV17_pCMV-MCP-ADAR2dd(E488Q)-NES

Plasmid

#200406

Purpose

Expresses a compact, engineered version of ADAR2 constitutively in mammalian cells

Depositor

James Collins

Article

Gayet et al Nat Commun. 2023 Mar 11;14(1):1339. doi:

Insert

MCP-ADAR2dd(E488Q)-NES

Use

Synthetic Biology

Expression

Mammalian

Mutation

E488Q (hyperactive)

Promoter

CMV

Available Since

June 30, 2023

Availability

Academic Institutions and Nonprofits only

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pZR060_CROPseq-sgCtrl-CRISPRi

Plasmid

#180269

Purpose

Lentiviral CROPseq vector expressing non-targeting control sgRNA for CRISPRi

Depositor

Alexander Marson

Article

Schmidt et al Science. 2022 Feb 4;375(6580):eabj4008.

Insert

No-Target Control sgRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Feb. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pHAGE2-EF1a-4xGFP11-SEC61B-IRES-Hygro

Plasmid

#211468

Purpose

Lentivirus backbone expressing SEC61B protein tagged with 4 tandem GFP11 repeats at the N-terminal

Depositor

Agnel Sfeir

Article

Fu et al Mol Cell. 2023 Oct 8:S1097-2765(23)00753

Insert

4xGFP11-SEC61B

Use

Lentiviral

Expression

Mammalian

Promoter

EF-1a

Available Since

Dec. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pHR_PGK_antiHer24D5-5_synNotch_Gal4VP64

Plasmid

#85423

Purpose

Lentiviral vector for expression of anti-Her2 scFv 24D5-5 Gal4VP64 synthetic Notch

Depositor

Wendell Lim

Article

Roybal et al Cell. 2016 Oct 6;167(2):419-432.e16. doi

Insert

anti-Her2_4D5-5 synNotch

Use

Lentiviral

Promoter

PGK

Available Since

Feb. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pSHDY-Prha-mVenus_rhaS

Plasmid

#137662

Purpose

Conjugative vector derived from pSHDY. Constitutive expression of the activator rhaS, as well as rhamnose-inducible expression of mVenus.

Depositor

Ilka Axmann

Article

Behle et al ACS Synth Biol. 2020 Apr 17;9(4):843-855

Insert

Prha:mVenus

Expression

Bacterial

Mutation

rhaS (Arg218Leu)

Promoter

Prha

Available Since

Feb. 26, 2020

Availability

Academic Institutions and Nonprofits only

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