We narrowed to 4,340 results for: Pet2

1. MEK1C218

   Plasmid

   #164639

   Purpose

   Bacterial expression plasmid for His6-MEK1C218

   Depositor

   Ben Davis

   Article

   Lai et al Cell. 2021 Apr 27. pii: S0092-8674(21)00

   Insert

   mitogen-activated protein kinase kinase 1 (MAP2K1 Human)
   

   Tags

   His6-tag

   Expression

   Bacterial

   Mutation

   G(-19)F/S218C/C277S/C376S

   Promoter

   T7

   Available Since

   May 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. DHFR-C-HiBiT

   Plasmid

   #207119

   Purpose

   Bacterially expressed DHFR-HiBiT for Ni-NTA purification

   Depositor

   James Inglese

   Article

   Ciulla et al Nat Commun. 2025 May 10;16(1):4342. doi:

   Insert

   Human-dihydrofolate reductase-HiBiT-10xHis (DHFR Human)
   

   Tags

   10xHis-tag and HiBiT tag

   Expression

   Bacterial

   Mutation

   Codon optmization for expression in BL21DE3

   Promoter

   T7

   Available Since

   May 13, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. *MtbCM-HiBiT-His

   Plasmid

   #228028

   Purpose

   Expresses secreted Mycobacterium tuberculosis (M. tb) chorismate mutase (CM) containing a C-terminal HiBiT sequence and His tag

   Depositor

   James Inglese

   Article

   van Neer et al ACS Infect Dis. 2025 Mar 14;11(3):703-71

   Insert

   Rv1885c Chorismate Mutase (Rv1885c Mycobacterium tuberculosis H37Rv)
   

   Tags

   10xHis-tag with thrombin site and HiBiT Tag

   Expression

   Bacterial

   Promoter

   T7 Promotor

   Available Since

   March 18, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. *MtbCM-bio-His

   Plasmid

   #186027

   Purpose

   Expresses secreted Mycobacterium tuberculosis (M. tb) chorismate mutase (CM) containing a C-terminal biotinylation site and His tag

   Depositor

   James Inglese

   Article

   van Neer et al ACS Infect Dis. 2025 Mar 14;11(3):703-71

   Insert

   Rv1885c Chorismate Mutase
   

   Tags

   Avi-tag for in vitro biotinylation and His-tag wi…

   Expression

   Bacterial

   Mutation

   Codon optimization for expression in BL21DE3

   Promoter

   T7 Promotor

   Available Since

   March 18, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. 8R6A

   Plasmid

   #228446

   Purpose

   Baculovirus expression for structure determination. May not contain entire coding region of gene.

   Depositor

   Cheryl Arrowsmith

   Article

   Munzker et al Commun Biol. 2024 Jul 24;7(1):901. doi:

   Insert

   DTX1 (DTX1 Human)
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Jan. 28, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET-hMICU1EF

   Plasmid

   #199180

   Purpose

   This plasmid is used to express a EF-hand disabled human MICU1 protein in E. coli. The protein is fused with a maltose binding protein in the N-terminus, and also contains a C-terminal His6 tag.

   Depositor

   Ming-Feng Tsai

   Article

   Tsai et al Proc Natl Acad Sci U S A. 2023 Apr 18;12

   Insert

   MICU1 (MICU1 Human)
   

   Tags

   His6 and MBP, Thrombin site, TEV site

   Mutation

   D421A, E432K, F433W, F453W

   Available Since

   Aug. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. His6-EGFP-cPHx1

   Plasmid

   #183674

   Purpose

   PtdIns(3,4)P2 biosensor for purification from bacteria

   Depositor

   Sergio Grinstein

   Article

   Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

   Insert

   PLEKHA1 (PLEKHA1 Human)
   

   Tags

   His6-EGFP

   Expression

   Bacterial

   Mutation

   Amino acids 169-329

   Promoter

   T7

   Available Since

   May 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. MEK1C218C222

   Plasmid

   #164640

   Purpose

   Bacterial expression plasmid for His6-MEK1C218C222

   Depositor

   Ben Davis

   Article

   Lai et al Cell. 2021 Apr 27. pii: S0092-8674(21)00

   Insert

   mitogen-activated protein kinase kinase 1 (MAP2K1 Human)
   

   Tags

   His6-tag

   Expression

   Bacterial

   Mutation

   G(-19)F/S218C/S222C/C277S/C376S

   Promoter

   T7

   Available Since

   May 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. mature ISG15 C-term. Domain (79-165, W123A)

   Plasmid

   #110765

   Purpose

   Bacterial expression of mutant proISG15 C-term. domain

   Depositor

   David Komander

   Article

   Swatek et al Proc Natl Acad Sci U S A. 2018 Mar 6;115

   Insert

   proISG15 C-term. domain (79-165, W123A) (ISG15 Human)
   

   Tags

   N-His6-3C

   Expression

   Bacterial

   Mutation

   W123A

   Promoter

   T7

   Available Since

   June 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. DOT1L-CC3

    Plasmid

    #111754

    Purpose

    Bacterial expression for structure determination

    Depositor

    Cheryl Arrowsmith

    Article

    Zhang et al Genes Dev. 2018 Mar 1;32(5-6):341-346. d

    Insert

    DOT1L (DOT1L Human)
    

    Tags

    8xHis-Thrombin-SUMO-TEV

    Expression

    Bacterial

    Mutation

    Contains coiled coil #3 (residues 613-661)

    Promoter

    T7

    Available Since

    June 14, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. DOT1L-CC1

    Plasmid

    #111756

    Purpose

    Bacterial expression for structure determination

    Depositor

    Cheryl Arrowsmith

    Article

    Zhang et al Genes Dev. 2018 Mar 1;32(5-6):341-346. d

    Insert

    DOT1L (DOT1L Human)
    

    Tags

    8xHis-Thrombin-SUMO-TEV

    Expression

    Bacterial

    Mutation

    contains coiled coil #1 (residues 506-555)

    Promoter

    T7

    Available Since

    June 14, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. DOT1L-CC2

    Plasmid

    #111755

    Purpose

    Bacterial expression for structure determination

    Depositor

    Cheryl Arrowsmith

    Article

    Zhang et al Genes Dev. 2018 Mar 1;32(5-6):341-346. d

    Insert

    DOT1L (DOT1L Human)
    

    Tags

    8xHis-Thrombin-SUMO-TEV

    Expression

    Bacterial

    Mutation

    Contains coiled coil #2 (residues 560-608)

    Promoter

    T7

    Available Since

    June 14, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. CCR5-SZ218a QTY

    Plasmid

    #162444

    Purpose

    Expression in E coli and perform ligand binding in solution

    Depositor

    Shuguang Zhang

    Article

    Qing et al iScience. 2020 Oct 28;23(12):101670. doi

    Insert

    C-C chemokine receptor type 5 (CCR5 Human)
    

    Tags

    his-tag

    Expression

    Bacterial

    Mutation

    All L, I, V, F in the transmembrane region mutate…

    Availability

    Academic Institutions and Nonprofits only

    

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14. CCR5-SZ190b QTY

    Plasmid

    #162445

    Purpose

    Expression in E coli and perform ligand binding in solution

    Depositor

    Shuguang Zhang

    Article

    Qing et al iScience. 2020 Oct 28;23(12):101670. doi

    Insert

    C-C chemokine receptor type 5 (CCR5 Human)
    

    Tags

    his-tag

    Expression

    Bacterial

    Mutation

    All L, I, V, F in the transmembrane region mutate…

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDL52 (clone76)

    Plasmid

    #188579

    Purpose

    High-yield production of coenzyme F420 in E. coli

    Depositor

    Gerald Lackner

    Article

    Last et al Metab Eng. 2022 Jul 18. pii: S1096-7176(

    Inserts

    ribA
    ribD
    yigB
    fbiC
    cofC
    cofD
    cofI
    cofE
    

    Expression

    Bacterial

    Mutation

    Codon optimization, synthetic ribosome binding si…

    Promoter

    T7 variants

    Available Since

    Sept. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. TRIP12-V2_A755-A841

    Plasmid

    #194759

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Article

    Munzker et al Commun Biol. 2024 Jul 24;7(1):901. doi:

    Insert

    TRIP12 (TRIP12 Human)
    

    Tags

    6xHis

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    July 19, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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17. pDL52 (clone45)

    Plasmid

    #188578

    Purpose

    High-yield production of coenzyme F420 in E. coli

    Depositor

    Gerald Lackner

    Article

    Last et al Metab Eng. 2022 Jul 18. pii: S1096-7176(

    Inserts

    ribA
    ribD
    yigB
    fbiC
    cofC
    cofD
    cofI
    cofE
    

    Expression

    Bacterial

    Mutation

    Codon optimization, synthetic ribosome binding si…

    Promoter

    T7 variants

    Available Since

    Sept. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. 6MIW

    Plasmid

    #127291

    Purpose

    Bacterial expression for structure determination. May not contain entire coding region of gene

    Depositor

    Cheryl Arrowsmith

    Article

    Munzker et al Commun Biol. 2024 Jul 24;7(1):901. doi:

    Insert

    HUWE1 (HUWE1 Human)
    

    Tags

    6xHis-TEV (MHHHHHHSSGRENLYFQG)

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    June 18, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. HisGB1-CITED2(216-269)+CBPTAZ1(340-439)

    Plasmid

    #173761

    Purpose

    Bacterial coexpression of human CITED2 CTAD with CBP TAZ1

    Depositor

    Peter Wright

    Article

    Berlow et al Nature. 2017 Mar 16;543(7645):447-451. d

    Inserts

    CITED2 (216-269) (CITED2 Human)
    CBP TAZ1 (340-439) (Crebbp Mouse)
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Feb. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pSumo24P7vB4

    Plasmid

    #113073

    Purpose

    Plasmid for highly efficient expression of engineered IL24 with binding affinity to cognate receptors

    Depositor

    Bohdan Schneider

    Article

    Zahradnik et al FEBS J. 2019 Oct;286(19):3858-3873. doi:

    Insert

    Human IL-24 engineered by computational design and fused with N-terminal SUMO tag (IL24 SUMO part (Brachypodium distachyon), Human)
    

    Tags

    SUMO

    Expression

    Bacterial

    Mutation

    Q60R, K62E, K68E, K77R, M80L, S88D, A89V, Q93R, Q…

    Promoter

    T7

    Available Since

    Sept. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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