We narrowed to 37,258 results for: uros

1. Direct-seq_lentiGuide-Puro-tRNA-Tetra Loop-8A8G

   Plasmid

   #157985

   Purpose

   Expresses programmed gRNA scaffold from U6 promoter. Programmed gRNA scaffold for streamlined scRNA-seq in CRISPR screen (Direct-seq). 3rd generation lentiviral backbone.

   Depositor

   Lijia Ma

   Article

   Song et al Genome Biol. 2020 Jun 8;21(1):136. doi:

   Insert

   S. pyogenes sgRNA cassette (tRNA-Tetra-8A8G)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   May 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLVX-CMV-Flag-PolB(K244A/T304I)-Puro

   Plasmid

   #177144

   Purpose

   Lentiviral vector expressing Flag-PolB(K244A/T304I) and a puromycin resistance cassette

   Depositor

   Robert Sobol

   Article

   Fang et al Nucleic Acids Res. 2019 Jul 9;47(12):626

   Insert

   PolB (POLB Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Dec. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBDKRB2_TCS(Q’L)_HA-dCas9(N)_P2A-Puro-WPRE

   Plasmid

   #101108

   Purpose

   Encodes dCas9(N) fused to BDKRB2

   Depositor

   Tudor Fulga

   Article

   Baeumler et al Cell Rep. 2017 Sep 12;20(11):2639-2653.

   Insert

   BDKRB2-dCas9(N)
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Flag and HA

   Expression

   Mammalian

   Available Since

   Nov. 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHAGE-IRES-puro-NLS-dHgm4Cas13b-EGFP-NLS

   Plasmid

   #191360

   Purpose

   Overexpression of NLS-dHgm4Cas13b-EGFP-NLS in human cells

   Depositor

   Ling-Ling Chen

   Article

   Yang et al Cell Insight. 2022 Jun 15;1(4):100044. d

   Insert

   NLS-dHgm4Cas13b-EGFP-NLS
   

   Tags

   EGFP

   Expression

   Mammalian

   Available Since

   Oct. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. AAV-puro-BGHpA-TRE-pEF-tagRFP-T-NLS

   Plasmid

   #163083

   Purpose

   Donor vector for integration of TagRFP-T reporter at AAVS1 safe harbor site via TALENs

   Depositor

   Lacra Bintu

   Article

   Van et al Nat Commun. 2021 Jan 22;12(1):537. doi:

   Insert

   TagRFP-T
   

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Jan. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. dnCEBP: pLenti-TRE-A-C/EBP-PGK-rtTAA-2A-Puro

   Plasmid

   #177201

   Purpose

   encoding a dominant negative form of CEBP under inducible promoter

   Depositor

   Markus Covert

   Article

   Kudo et al bioRxiv (2022)

   Insert

   dnCEBP (A-C/EBP)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   TRE

   Available Since

   Aug. 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLKO.1-puro U6 N. meningitidis sgRNA BfuAI large stuffer

   Plasmid

   #86195

   Purpose

   U6 based expression of N meningitidis sgRNA

   Depositor

   Scot Wolfe

   Article

   Amrani et al Genome Biol. 2018 Dec 5;19(1):214. doi:

   Insert

   nmCas9 sgRNA cloning cassete
   

   Use

   Lentiviral

   Promoter

   U6

   Available Since

   Jan. 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCXCR4_TCS(Q’L)_HA-dCas9(N)_P2A-Puro-WPRE

   Plasmid

   #101114

   Purpose

   Encodes dCas9(N) fused to CXCR4

   Depositor

   Tudor Fulga

   Article

   Baeumler et al Cell Rep. 2017 Sep 12;20(11):2639-2653.

   Insert

   CXCR4-dCas9(C)-VP64
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Flag and HA

   Expression

   Mammalian

   Available Since

   Nov. 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pAVPR2_TCS(Q’L)_HA-dCas9(N)_P2A-Puro-WPRE

   Plasmid

   #101112

   Purpose

   Encodes dCas9(N) fused to AVPR2

   Depositor

   Tudor Fulga

   Article

   Baeumler et al Cell Rep. 2017 Sep 12;20(11):2639-2653.

   Insert

   AVPR2-dCas9(N)
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Flag and HA

   Expression

   Mammalian

   Available Since

   Nov. 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pVEGFR2_TEV(N)_NES_TCS(Q'L)_FKBP_HA-dCas9(N)_P2A_Puro-WPRE

    Plasmid

    #101106

    Purpose

    Encodes FKBP-dCas9(N) fused to VEGFR2

    Depositor

    Tudor Fulga

    Article

    Baeumler et al Cell Rep. 2017 Sep 12;20(11):2639-2653.

    Insert

    VEGFR2-FKBP-dCas9(N)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    HA

    Expression

    Mammalian

    Available Since

    Nov. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLPAR1_TCS(Q’L)_HA-dCas9(N)_P2A-Puro-WPRE

    Plasmid

    #101116

    Purpose

    Encodes dCas9(N) fused to LPAR1

    Depositor

    Tudor Fulga

    Article

    Baeumler et al Cell Rep. 2017 Sep 12;20(11):2639-2653.

    Insert

    LPAR1-dCas9(N)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Flag and HA

    Expression

    Mammalian

    Available Since

    Nov. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. hPlekhm1-Mid/pIRES puro Glue (Q513-E784)

    Plasmid

    #73455

    Purpose

    Express human middle part (Q513-E784) Plekhm1 in mammalian cells

    Depositor

    Paul Odgren

    Article

    Witwicka et al PLoS One. 2015 May 19;10(5):e0127537. do

    Insert

    Plekhm1 (PLEKHM1 Human)
    

    Tags

    CBP tag, HA tag, and strep tag

    Expression

    Mammalian

    Available Since

    March 14, 2016

    Availability

    Academic Institutions and Nonprofits only

13. pLV-Puro-CMV-EPB41L4A-AS1-unspliced MUT

    Plasmid

    #242750

    Purpose

    Expresses unspliced EPB41L4A-AS1 in mammalian cells

    Depositor

    Igor Ulitsky

    Article

    Monziani et al bioRxiv 2025.04.08.647803

    Insert

    EPB41L4A-AS1 (EPB41L4A-AS1 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    AACTTAAAAGCAGCGT → ACCTTAGAAGTAGAGT making it res…

    Promoter

    CMV

    Available Since

    Sept. 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLex307-UCOE-SFFV-puroR-MCP-HSF1-VIRF2

    Plasmid

    #218557

    Purpose

    Encodes SFFV promoter-driven MCP-HSF1-VIRF2 with an N-terminal bipartite SV40 NLS, along with puromycin resistance

    Depositor

    Alejandro Chavez

    Insert

    MCP-HSF1-VIRF2
    

    Use

    Lentiviral

    Tags

    SV40NLS

    Expression

    Mammalian

    Mutation

    Cas9 mutations to make dCas9=D10A+D839A+H840A+N86…

    Available Since

    Aug. 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLex307-UCOE-SFFV-puroR-MCP-MLLx3-HSF1

    Plasmid

    #218558

    Purpose

    Encodes SFFV promoter-driven MCP-MLLx3-HSF1 with an N-terminal bipartite SV40 NLS, along with puromycin resistance

    Depositor

    Alejandro Chavez

    Insert

    MCP-MLLx3-HSF1
    

    Use

    Lentiviral

    Tags

    SV40NLS

    Expression

    Mammalian

    Mutation

    Cas9 mutations to make dCas9=D10A+D839A+H840A+N86…

    Available Since

    Aug. 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLex307-UCOE-SFFV-puroR-MCP-P65-HSF1

    Plasmid

    #218559

    Purpose

    Encodes SFFV promoter-driven MCP-P65-HSF1 with an N-terminal bipartite SV40 NLS, along with puromycin resistance

    Depositor

    Alejandro Chavez

    Insert

    MCP-P65-HSF1
    

    Use

    Lentiviral

    Tags

    SV40NLS

    Expression

    Mammalian

    Mutation

    Cas9 mutations to make dCas9=D10A+D839A+H840A+N86…

    Available Since

    Aug. 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pRCA905 - KLF4 Puro-T2A-BFP (pRCA847 backbone)

    Plasmid

    #238185

    Purpose

    Lentiviral vector for overexpression KLF4 ORF from a EF1a promoter

    Depositor

    Thomas Norman

    Article

    Southard et al Nat Genet. 2025 Aug 6. doi: 10.1038/s415

    Insert

    KLF4 (KLF4 Human, Synthetic)
    

    Use

    Lentiviral

    Tags

    mTagBFP2

    Available Since

    June 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pRCA881 - KLF4 Puro-T2A-GFP (pRCA847 backbone)

    Plasmid

    #238182

    Purpose

    Lentiviral vector for overexpression KLF4 ORF from a EF1a promoter

    Depositor

    Thomas Norman

    Article

    Southard et al Nat Genet. 2025 Aug 6. doi: 10.1038/s415

    Insert

    KLF4 (KLF4 Human, Synthetic)
    

    Use

    Lentiviral

    Tags

    GFP

    Available Since

    June 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. LSM0251 (PB)EF1a-FKBP-tm-ADAR2ddIRESv0-PuroR-P2A-BFP

    Plasmid

    #233442

    Purpose

    PiggyBac donor plasmid to overexpress FKBP-TM-ADAR2dd (eLIDAR)

    Depositor

    Xiaojing Gao

    Article

    Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

    Insert

    FKBP-tm-ADAR2ddIRESv0-PuroR-P2A-BFP
    

    Expression

    Mammalian

    Mutation

    E488Q, T501A on ADAR2dd

    Promoter

    EF1a

    Available Since

    April 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCAGGS puro -GFP-delta73-82 REX1-IRES-mCherry

    Plasmid

    #232246

    Purpose

    Mammalian expression of delta73-82 REX1-GFP-IRES-mCherry

    Depositor

    Francisco Bustos

    Article

    Bandi et al HGG Adv. 2024 Oct 31;6(1):100378. doi: 1

    Insert

    delta73-82 REX1-GFP-IRES-mCherry (Zfp42 Mouse)
    

    Tags

    AcGFP1

    Expression

    Mammalian

    Mutation

    delta73-82

    Available Since

    Feb. 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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